Skip to main content
SpringerLink
Log in
Book cover

Mechanism of Action of Antibacterial Agents pp 329–343Cite as

Thiostrepton and Related Antibiotics

  • Chapter

Part of the book series: Antibiotics ((1512,volume 5 / 1))

Abstract

The antibiotics, thiostrepton, siomycin, thiopeptin and sporangiomycin, are grouped here since they are similar compounds and appear to share a common mode of action. Thus, to date, these compounds have not been distinguished one from another in any assay of their modes of action nor have any bacterial mutant strains been shown to be resistant to one, but not all, members of the group. However, since these are not identical compounds, it remains possible that, in time, this grouping may become redundant; also it remains to be seen whether other compounds could be included.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Ballesta, J.P.G., Vazquez, D.: Elongation factor T-dependent hydrolysis of guanosine triphosphate resistant to thiostrepton. Proc. Natl. Acad. Sci. USA 69, 3058–3062 (1972 a)

    Article  PubMed  CAS  Google Scholar 

  • Ballesta, J.P.G., Vazquez, D.: Reconstitution of the 50S ribosome subunit. Role of proteins L7 and L12 in the GTPase activities. Site of action of thiostrepton. FEBS Lett. 28, 337–342 (1972 b)

    Article  PubMed  CAS  Google Scholar 

  • Bazzicalupo, M., Parisi, B., Pirali, G., Polsinelli, M., Sala, F.: Genetic and biochemical characterization of a ribosomal mutant of Bacillus subtilis resistant to sporangiomycin. Antimicrob. Agents Chemother. 8, 651–656 (1975)

    PubMed  CAS  Google Scholar 

  • Bernabeu, C., Vazquez, D., Ballesta, J.P.G.: Activities of protein-deficient particles derived from 50S ribosomal subunits by NH4Cl/Ethanol treatment. Eur. J. Biochem. 69, 233–241 (1976)

    Article  PubMed  CAS  Google Scholar 

  • Bodley, J.W., Lin, L., Highland, J.H.: Studies on translocation VI: Thiostrepton prevents the formation of a ribosome — G factor-guanine nucleotide complex. Biochem. Biophys. Res. Commun. 41, 1406–1411 (1970 a)

    Article  PubMed  CAS  Google Scholar 

  • Bodley, J.W., Zieve, F.J., Lin, L., Zieve, S.T.: Studies on translocation III: Conditions necessary for the formation and detection of a stable ribosome — G factor — guanosine diphosphate complex in the presence of fusidic acid. J. Biol. Chem. 245, 5656–5661 (1970 b)

    PubMed  CAS  Google Scholar 

  • Brot, N., Yamasaki, E., Redfield, B., Weissbach, H.: The properties of an E. coli ribosomal protein required for the function of factor G. Arch. Biochem. Biophys. 148, 148–155 (1972)

    Article  PubMed  CAS  Google Scholar 

  • Brot, N., Tate, W.P., Caskey, C.T., Weissbach, H.: The requirement for ribosomal proteins L7 and L12 in polypeptide chain termination. Proc. Natl. Acad. Sci. USA 71, 89–92 (1974)

    Article  PubMed  CAS  Google Scholar 

  • Cabrer, B., Vazquez, D., Modelell, J.: Inhibition by elongation factor G of aminoacyl-tRNA binding to ribosomes. Proc. Natl. Acad. Sci. USA 69, 733–736 (1972)

    Article  PubMed  CAS  Google Scholar 

  • Cannon, M., Burns, K.: Modes of action of erythromycin and thiostrepton as inhibitors of protein synthesis. FEBS Lett. 18, 1–5 (1971)

    Article  PubMed  CAS  Google Scholar 

  • Celma, M.L., Vazquez, D., Modolell, J.: Failure of fusidic acid and siomycin to block ribosomes in the pretranslocated state. Biochem. Biophys. Res. Commun. 48, 1240–1246 (1972)

    Article  PubMed  CAS  Google Scholar 

  • Cundliffe, E.: The mode of action of thiostrepton in vivo. Biochem. Biophys. Res. Commun. 44, 912–917 (1971)

    Article  PubMed  CAS  Google Scholar 

  • Cundliffe, E.: Antibiotic inhibitors of ribosome function. In: Molecular basis of antibiotic action. Gale, E.F., Cundliffe, E., Reynolds, P.E., Richmond, M.H., Waring, M.J. (eds.), pp. 278–379. John Wiley and Sons 1972 a

    Google Scholar 

  • Cundliffe, E.: The mode of action of fusidic acid. Biochem. Biophys. Res. Commun. 46, 1974–1801 (1972 b)

    Article  Google Scholar 

  • Cundliffe, E., Dixon, P.D.: Inhibition of ribosomal A site functions by sporangiomycin and micrococcin. Antimicrob. Agents Chemother. 8, 1–4 (1975)

    PubMed  CAS  Google Scholar 

  • Cundliffe, E., Beven, J.E., Dixon, P.D.: Ribosomal effects of thiostrepton and related antibiotics. In: Topics in infectious diseases. Drews, T., Hahn, F.E. (eds.), Vol. I, pp. 167–177. Berlin-Heidelberg-New York: Springer 1975

    Google Scholar 

  • Dixon, P.D.: Ph.D. dissertation, Univ. of Cambridge (1976)

    Google Scholar 

  • Dixon, P.D., Beven, J.E., Cundliffe, E.: Properties of the ribosomes of antibiotic producers: Effects of thiostrepton and micrococcin on the organisms which produce them. Antimicrob. Agents Chemother. 7, 850–855 (1975)

    PubMed  CAS  Google Scholar 

  • Gaunt-Klöpfer, M., Erdmann, V.A.: ATPase and GTPase activities associated with the 5S RNA-protein complex of E. coli ribosomes. Biochim. Biophys. Acta. 390, 226–230 (1975)

    PubMed  Google Scholar 

  • Goldthwaite, C., Smith, I.: Physiological characterization of antibiotic-resistant mutants of Bacillus subtilis. Mol. Gen. Genet. 114, 190–204 (1972)

    Article  PubMed  CAS  Google Scholar 

  • Goldthwaite, C., Dubnau, D., Smith, I.: Genetic mapping of antibiotic resistance markers in Bacillus subtilis. Proc. Natl. Acad. Sci. USA 65, 96–103 (1970)

    Article  PubMed  CAS  Google Scholar 

  • Hamel, E., Koka, M., Nakamoto, T.: Requirement of an E. coli 50S ribosomal protein component for effective interaction of the ribosome with T and G factors and with guanosine triphosphate. J. Biol. Chem. 247, 805–814 (1972)

    PubMed  CAS  Google Scholar 

  • Harford, N., Sueoka, N.: Chromosomal location of antibiotic resistance markers in Bacillus subtilis. J. Mol. Biol. 51, 267–286 (1970)

    Article  PubMed  CAS  Google Scholar 

  • Highland, J.H., Lin, L., Bodley, J.W.: Studies on translocation VIII: Protection of ribosomes from thiostrepton inactivation by the binding of G factor and GDP. Biochemistry 10, 4404–4409 (1971)

    Article  PubMed  CAS  Google Scholar 

  • Highland, J.H., Howard, G.A., Gordon, J.: Binding of thiostrepton to the ribosomes of E. coli: Characterization and stoichiometry of binding. Eur. J. Biochem. 53, 313–318 (1975 a)

    Article  CAS  Google Scholar 

  • Highland, J.H., Howard, G.A., Ochsner, E., Stöffler, G., Hasenbank, R., Gordon, J.: Identification of a ribosomal protein necessary for thiostrepton binding to E. coli ribosomes. J. Biol. Chem. 250, 1141–1145 (1975 b)

    PubMed  CAS  Google Scholar 

  • Hörne, J.R., Erdmann, V.A.: Effects of ethanol, methanol and different anbibiotics on the ATPase and GTPase activities associated with B. stearothermophilus 5S RNA-protein complexes. FEBS Lett. 42, 42–45 (1974)

    Article  PubMed  Google Scholar 

  • Kinoshita, T., Liou, Y-F., Tanaka, N.: Inhibition by thiopeptin of ribosomal functions associated with T and G factors. Biochem. Biophys. Res. Commun. 44, 859–963 (1971)

    Article  PubMed  CAS  Google Scholar 

  • Kischa, K., Möller, W., Stöffler, G.: Reconstitution of a GTPase activity by a 50S ribosomal protein from E. coli. Nature New Biol. 233, 62–63 (1971)

    Article  PubMed  CAS  Google Scholar 

  • Liou, Y-F., Kinoshita, T., Tanaka, N., Yoshikawa, M.: Studies on the ribosomes of a thiopeptin-resistant mutant of E. coli. J. Antibiot. 26, 711–716 (1973)

    PubMed  CAS  Google Scholar 

  • Liou, Y-F., Yoshikawa, M., Tanaka, N.: Alteration of ribosomal protein L5 in a thiopeptin-resistant mutant of E. coli. Biochem. Biophys. Res. Commun. 65, 1096–1101 (1975)

    Article  PubMed  CAS  Google Scholar 

  • Lockwood, A.H., Sarkar, P., Maitra, U., Brot, N., Weissbach, H.: Effects of thiostrepton on polypeptide chain initiation in E. coli. J. Biol. Chem. 249, 5831–5834 (1974)

    PubMed  CAS  Google Scholar 

  • Miller, D.L.: Elongation factors EF-Tu and EF-G interact at related sites on ribosomes. Proc. Natl. Acad. Sci. USA 69, 752–755 (1972)

    Article  PubMed  CAS  Google Scholar 

  • Modolell, J., Vazquez, D., Monro, R.E.: Ribosomes, G factor and siomycin. Nature New Biol. 230, 109–112 (1971 a)

    Article  PubMed  CAS  Google Scholar 

  • Modolell, J., Cabrer, B., Parmeggiani, A., Vazquez, D.: Inhibition by siomycin and thiostrepton of both aminoacyl-tRNA and factor G binding to ribosomes. Proc. Natl. Acad. Sci. USA 68, 1796–1800 (1971 b)

    Article  PubMed  CAS  Google Scholar 

  • Naaktgeboren, N., Roobol, K., Gubbens, J., Voorma, H.O.: The mode of action of thiostrepton in the initiation of protein synthesis. Eur. J. Biochem. 70, 39–47 (1976)

    Article  PubMed  CAS  Google Scholar 

  • Pestka, S.: Thiostrepton. A ribosomal inhibitor of translocation. Biochem. Biophys. Res. Commun. 40, 667–614 (1970)

    Article  PubMed  CAS  Google Scholar 

  • Pestka, S., Bodley, J.W.: The thiostrepton group of antibiotics. In: Antibiotics. Mode of action. Corcoran, J.W., Hahn, F.E. (eds.), Vol. III, pp. 551–573. Berlin, Heidelberg, New York: Springer 1975

    Google Scholar 

  • Pestka, S., Weiss, D., Vince, R., Wienen, B., Stöffler, G., Smith, I.: Thiostrepton — resistant mutants of Bacillus subtilis: Localization of resistance to the 50S subunit. Mol. Gen. Genet. 144, 235–241 (1976)

    Article  PubMed  CAS  Google Scholar 

  • Pirali, G., Lancini, G.C., Parisi, B., Sala, F.: Interaction of sporangiomycin with the bacterial ribosome. J. Antibiot. 25, 561–568 (1972)

    PubMed  CAS  Google Scholar 

  • Richman, N., Bodley, J.W.: The sites on the 50S ribosomal subunit with which elongation factors Tu and G interact are at least partially identical. Proc. Natl. Acad. Sci. USA 69, 686–689 (1972)

    Article  PubMed  CAS  Google Scholar 

  • Richter, D.: Inability of E. coli ribosomes to interact simultaneously with the bacterial elongation factors EF-Tu and EF-G. Biochem. Biophys. Res. Commun. 46, 1850–1856 (1972)

    Article  PubMed  CAS  Google Scholar 

  • Sarkar, P., Stringer, E.A., Maitra, U.: Thiostrepton inhibition of initiation factor 1 activity in polypeptide chain initiation in E. coli. Proc. Natl. Acad. Sci. USA 71, 4986–4990 (1974)

    Article  PubMed  CAS  Google Scholar 

  • Schrier, P.I., Möller, W.: The involvement of 50S ribosomal protein L11 in the EF G — dependent GTP hydrolysis of E. coli ribosomes. FEBS Lett. 54, 130–134 (1975)

    Article  PubMed  CAS  Google Scholar 

  • Sopori, M.L., Lengyel, P.: Components of the 50S ribosomal subunit involved in GTP cleavage. Biochem. Biophys. Res. Commun. 46, 238–244 (1972)

    Article  PubMed  CAS  Google Scholar 

  • Tanaka, K., Watanabe, S., Tamaki, M.: Mode of action of siomycin. J. Antibiot. 23, 13–19 (1970 a)

    PubMed  CAS  Google Scholar 

  • Tanaka, K., Watanabe, S., Teraoka, H., Tamaki, M.: Effect of siomycin on protein synthesizing activity of E. coli ribosomes. Biochem. Biophys. Res. Commun. 39, 1189–1193 (1970 b)

    Article  PubMed  CAS  Google Scholar 

  • Tate, W.P., Beaudet, A.L., Caskey, C.T.: Guanine nucleotides and elongation factors, influence on interaction of release factors with ribosomes. Proc. Natl. Acad. Sci. USA 70, 2350–2352 (1973)

    Article  PubMed  CAS  Google Scholar 

  • Tiboni, O., Ciferri, O.: Selective inhibition of the reactions catalysed by ribosome-specific transfer factors. FEBS Lett. 19, 174–179 (1971)

    Article  PubMed  CAS  Google Scholar 

  • Vince, R., Weiss, D., Gordon, J., Howard, G., Smith, I., Pestka, S.: Binding of thiostrepton to ribosomes from thiostrepton-sensitive and -resistant Bacillus subtilis strains. Antimicrob. Agents Chemother. 9, 665–667 (1976)

    PubMed  CAS  Google Scholar 

  • Weisblum, B., Demohn, V.: Thiostrepton, an inhibitor of 50S ribosome subunit function. J. Bacteriol. 101, 1073–1074 (1970 a)

    PubMed  CAS  Google Scholar 

  • Weisblum, B., Demohn, V.: Inhibition by thiostrepton of the formation of a ribosome-bound guanine nucleotide complex. FEBS Lett. 11, 149–152 (1970 b)

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. E. Cundliffe
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of the Army, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington D.C., 20012, USA

    Fred E. Hahn Ph.D.

Rights and permissions

Reprints and permissions

Copyright information

© 1979 Springer-Verlag Berlin · Heidelberg

About this chapter

Cite this chapter

Cundliffe, E. (1979). Thiostrepton and Related Antibiotics. In: Hahn, F.E. (eds) Mechanism of Action of Antibacterial Agents. Antibiotics, vol 5 / 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-46403-4_18

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-46403-4_18

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-46405-8

  • Online ISBN: 978-3-642-46403-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation