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Topics and Perspectives in Adenosine Research pp 470–479Cite as

Endothelial Activation by Adenosine and Coronary Flow Regulation in the Guinea Pig Heart

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Summary

In order to investigate the relationship between receptor-mediated activation of coronary endothelial adenylate cyclase and changes in coronary flow, coronary endothelial adenine nucleotides of isolated guinea pig hearts were selectively prelabeled by an intracoronary infusion of 3H-adenosine at a concentration of 10−8 M. Following prelabeling, the release of 3H-cAMP into the coronary effluent perfusate was measured and used as an index of the activation of endothelial adenylate cyclase. Adenosine, NECA, R-PIA, and S-PIA caused parallel increases in coronary conductance and the coronary efflux of 3H-cAMP, and both effects showed a rank order of potency characteristic of adenosine A2 receptors. Using the specific radioactivity of cyclic AMP released during NECA stimulation to represent the specific radioactivity of endothelial precursor adenine nucleotides, the contribution of the coronary endothelium to total cardiac adenosine release was calculated to be 14%. Procaterol, a selective beta2 adrenoceptor agonist, and prostaglandin E1 also caused coronary vasodilation and increased 3H-cAMP release without augmenting left ventricular contractile function. In contrast to these compounds, ATP caused near maximal vasodilation, but 3H-cAMP release remained near control levels. These results demonstrate the presence of adenosine A2, beta2 adrenergic, and prostaglandin E1 receptors that stimulate coronary endothelial adenylate cyclase. In addition, the data suggest the presence of a distinct purinergic receptor that is involved in coronary vasodilation. Activation of coronary endothelial adenylate cyclase may play a minor role in mediating coronary vasodilation.

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Authors and Affiliations

  1. Physiological Institute I, University of Düsseldorf, Moorenstraße 5, D-4000, Düsseldorf 1, Germany

    K. Kroll, J. Schrader & D. Möllmann

Authors
  1. K. Kroll
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  2. J. Schrader
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  3. D. Möllmann
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Editors and Affiliations

  1. Physiologisches Institut, Universität München, Pettenkoferstraße 12, 8000, München 2, Federal Republic of Germany

    Eckehart Gerlach  & Bernhard Friedrich Becker  & 

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© 1987 Springer-Verlag Berlin Heidelberg

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Kroll, K., Schrader, J., Möllmann, D. (1987). Endothelial Activation by Adenosine and Coronary Flow Regulation in the Guinea Pig Heart. In: Gerlach, E., Becker, B.F. (eds) Topics and Perspectives in Adenosine Research. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-45619-0_39

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  • DOI: https://doi.org/10.1007/978-3-642-45619-0_39

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-45621-3

  • Online ISBN: 978-3-642-45619-0

  • eBook Packages: Springer Book Archive

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