Summary
Under physiological conditions, concentrations of adenosine in arterial and venous blood are very similar, although the nucleoside is rapidly metabolized by blood cells and the vascular endothelium. In order to characterize the possible regulatory role of endothelial cells in the homeostasis of adenosine in the blood, studies concerning metabolism of adenosine and adenine nucleotides were carried out on cultured endothelial cells of various origin and on different vessel preparations.
Micro- and macrovascular endothelial cells are capable of both a continuous uptake and release of adenosine. Adenosine taken up can be incorporated into adenine nucleotides or catabolized, the relative proportions depending on its concentration. Adenosine released from the endothelium is preferentially derived from the breakdown of adenine nucleotides. All endothelial cells exhibit extraordinarily active ectonucleotidases (ATPase, ADPase, 5′-nucleotidase), whereby also extracellular nucleotides are rapidly degraded to adenosine. This adenosine can accumulate extracellularly, mainly in cultures of macrovascular endothelial cells owing to their slower rate of adenosine uptake and metabolism. Similar observations pertain to isolated perfused segments of rabbit caval veins with intact endothelium. In vessel preparations denuded of endothelium extracellular adenine nucleotide degradation yields far more inosine than adenosine.
The net production of adenosine from intra- and extracellular adenine nucleotides by the vascular endothelium in vivo must exceed endothelial uptake of the nucleoside. Otherwise, similar arterial and venous plasma levels could not be maintained, since adenosine is also taken up and metabolized by red blood cells. Based on these considerations one has to postulate a concentration gradient of adenosine to exist between the unstirred plasma layer at the endothelial surface and the central blood stream. We propose that adenosine of endothelial origin — intra- and extracellularly formed and highly concentrated at the luminal surface — may constitute an important antiaggregatory mechanism, which is part of the well-known antithrombogenicity of an intact endothelial lining.
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References
Becker BF, Gerlach E (1985) Uric acid formed in the coronary endothelium is the major adenine nucleotide catabolite released from isolated perfused guinea pig hearts. Pflügers Arch 405: Suppl 2, R10
Becker BF, Gerlach E (1986) Uric acid, the major adenine nucleotide catabolite released from isolated perfused guinea pig hearts, is formed in the coronary endothelium. J Mol Cell Cardiol 18: Suppl 1, 157
Becker BF, Gerlach E (1986) Uric acid, the major catabolite of cardiac adenine nucleotides and adenosine, originates in the coronary endothelium. This volume, pp 209-223
Böck M, Möller A, Nees S, Gerlach E (1984) Extracellular degradation of adenine nucleotides by coronary endothelial cells and vascular endothelium of other origin. Pflügers Arch 402: Suppl R20
Böck M, Nees S, Möller A, Gerlach E (1985) Extrazellulärer Abbau von Adeninnukleotiden an Endothelzellen aus verschiedenen Gefäßabschnitten und im Vollblut. Z Kardiol 74: Suppl 3,20
Born GVR, Cross MJ (1963) The aggregation of blood platelets. J Physiol 168:178–195
Cronstein BN, Levin RI, Belanoff J, Weissmann G, Hirschhorn R (1986) A new function for adenosine: protection of vascular endothelial cells from neutrophil-mediated injury. This volume, pp 299-308
Crutchley DJ, Ryan US, Ryan JW (1980) Effects of aspirin and dipyridamole on the degradation of adenosine diphosphate by cultured cells derived from bovine pulmonary artery. J Clin Invest 66:29–35
Des Rosiers C, Nees S, Gerlach E (1985) Purin-Stoffwechsel in kultivierten Endothelzellen verschiedener vaskulärer Herkunft. Z Kardiol 74: Suppl. 3,67
Deussen A, Möser G, Schrader J (1986) Contribution of coronary endothelial cells to cardiac adenosine production. Pflügers Arch 406:608–614
Dosne AM, Bodevin E (1983) Purine release from [14C]adenine-labelled endothelial cells: reduction by dipyridamole. Mol Physiol 3:175–181
Gerlach E, Nees S, Becker BF (1985) The vascular endothelium: a survey of some newly evolving biochemical and physiological features. Basic Res Cardiol 80:459–474
Hellewell PG, Pearson JD (1983) Metabolism of circulating adenosine by the porcine isolated perfused lung. Circ Res 53:1–7
Jaffe EA (1985) Physiologic functions of normal endothelial cells. In: Lee KT (ed) Atherosclerosis. Ann NY Acad Sci 454:279–291
Jarasch ED, Grund C, Bruder G, Heid HW, Keenan TW, Franke WW (1981) Localization of xanthine oxidase in mammary gland epithelium and capillary endothelium. Cell 25:67–82
Nees S, Gerlach E (1983) Adenine nucleotides and adenosine metabolism in cultured coronary endothelial cells: formation and release of adenine compounds and possible functional implications. In: Berne RM, Rall TN, Rubio R (eds) Regulatory function of adenosine. NijhofT, Boston, pp 347–355
Nees S, Gerbes AL, Gerlach E (1981) Isolation, identification, and continuous culture of coronary endothelial cells from guinea pig hearts. Eur J Cell Biol 24:287–297
Nees S, Böck M, Herzog V, Becker BF, Des Rosiers C, Gerlach E (1985) The adenine nucleotide metabolism of the coronary endothelium: implications for the regulation of coronary flow by adenosine. In: Stefanovich V, Rudolphi K, Schubert P (eds) Adenosine: receptors and modulation of cell function. IRL, Oxford-Washington DC, pp 419–436
Nees S, Herzog V, Becker BF, Böck M, Des Rosiers C, Gerlach E (1985) The coronary endothelium: a highly active metabolic barrier for adenosine. Basic Res Cardiol 80:515–529
Pearson JD, Gordon JL (1985) Nucleotide metabolism by endothelium. Annu Rev Physiol 47:617–627
Pearson JD, Coade S (1986) Kinetics of endothelial cell ectonucleotidases. This volume, pp 145-154
Pearson JD, Carleton JS, Gordon JL (1980) Metabolism of adenine nucleotides by ectoenzy-mes of vascular endothelial and smooth-muscle cells in culture. Biochem J 190:421–429
Plageman PGW, Wohlhüter RM, Kraupp M (1985) Adenosine uptake, transport, and metabolism in human erythrocytes. J Cell Physiol 125:330–336
Ryan US (1986) Metabolic activity of pulmonary endothelium: modulations of structure and function. Annu Rev Physiol 48:263–272
Sollevi A, Lagerkranser M, Andreen M, Irestedt L (1984) Relationship between arterial and venous adenosine levels and vasodilatation during ATP-and adenosine-infusion in dogs. Acta Physiol Scand 120:171–176
Sollevi A, Torsell L, Öwall A, Edlund A, Lagerkranser M (1986) Levels and cardiovascular effects of adenosine in humans. This volume, pp 599-613
Stiegler H, Klug M, Nees S (1986) Metabolism of adenine nucleotides and adenosine in isolated perfused v. cava segments of rabbits. Pflügers Arch 407: Suppl 1, S40
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Gerlach, E., Becker, B.F., Nees, S. (1987). Formation of Adenosine by Vascular Endothelium: a Homeostatic and Antithrombogenic Mechanism?. In: Gerlach, E., Becker, B.F. (eds) Topics and Perspectives in Adenosine Research. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-45619-0_25
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DOI: https://doi.org/10.1007/978-3-642-45619-0_25
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