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Pericytes: a Ubiquitous Source of Multipotent Adult Tissue Stem Cells

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Stem Cells in Aesthetic Procedures

Abstract

There is a perivascular reservoir of stem/progenitor cells for cellular therapies. Bone marrow- and adipose-derived mesenchymal progenitor/stem cells possess great promise for regenerative medicine, immunomodulation, and cancer-targeting therapies. The authors describe working toward a definition of the pericyte and discuss putative markers that identify pericytes. These markers include alpha smooth muscle actin (αSMA) and desmin expression, neuron-glial 2 (NG2) and nestin expression, expression of CD146 and CD106, and expression of MSC markers and the mucosialin CD34. The authors then go into the developmental origins of pericytes. Pericytes may prove to be promising tools for future regenerative applications because they represent a ubiquitous source of mesenchymal progenitors. However, they are comprised of a heterogeneous mixture of specialized cells that possess phenotypic variability among tissues and even throughout the vascular tree.

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Acknowledgments

This work was supported by grants BC032981 and BC044784 from the Department of Defense, grant R01CA 114246 from the NIH, grant R01-HL-085819 from the National Heart, Lung, and Blood Institute, the Hillman Foundation, the Glimmer of Hope Foundation, the Commonwealth of Pennsylvania, through the McGowan Institute of Regenerative Medicine, the NHLBI (Production Assistance for Cellular Therapy (PACT) N01-HB-37165), and the Department of Defense Biomedical Translational Initiative (W911QY-09-C-0209). We would also like to thank Diana Napper from The Glimmer of Hope Foundation for her support. The UPCI Cytometry Facility is supported by CCSG P30CA047904. Flow cytometry data were collected with the technical assistance of Melanie E. Pfeifer and E. Michael Meyer. Dr. Zambidis and Dr. Park were supported by grants from NIH 1U01HL099775 and U01HL100397 (ETZ) and the Maryland Stem Cell Research Fund: 2011-MS CRF II-0008-00 and 2007-MSCRF II-0379-00 (ETZ) and the Maryland Stem Cell Research Fund Postdoctoral Fellowship grant 2009-MSCRF III-106570 (TSP).

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Authors and Affiliations

  1. Division of Pediatric Oncology, Johns Hopkins University, Department of Oncology, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, 733 N. Broadway, MRB 772, Baltimore, MD, 21205, USA

    Ludovic Zimmerlin Ph.D., Tea Soon Park Ph.D. & Elias T. Zambidis M.D., Ph.D.

  2. Institute for Cell Engineering, Johns Hopkins School of Medicine, Baltimore, MD, USA

    Ludovic Zimmerlin Ph.D., Tea Soon Park Ph.D. & Elias T. Zambidis M.D., Ph.D.

  3. Department of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Research Pavilion 2.42b, 5117 Centre Avenue, Pittsburgh, PA, 15213, USA

    Vera S. Donnenberg Ph.D

  4. University of Pittsburgh Cancer Institute, Hillman Cancer Center, Pittsburgh, PA, USA

    Vera S. Donnenberg Ph.D & Albert D. Donnenberg Ph.D

  5. McGowan Institute for Regenerative Medicine, Pittsburgh, PA, USA

    Vera S. Donnenberg Ph.D & Albert D. Donnenberg Ph.D

  6. Division of Hematology/Oncology, Department of Medicine, University of Pittsburgh School of Medicine, Research Pavilion 2.42c, 5117 Centre Avenue, Pittsburgh, PA, 15213, USA

    Albert D. Donnenberg Ph.D

  7. Hematopoietic Stem Cell Laboratory, University of Pittsburgh Medical Center, Pittsburgh, PA, USA

    Albert D. Donnenberg Ph.D

  8. University of Pittsburgh Cancer Institute Cytometry Facility, Pittsburgh, PA, USA

    Albert D. Donnenberg Ph.D

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  1. Ludovic Zimmerlin Ph.D.
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  2. Tea Soon Park Ph.D.
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  3. Vera S. Donnenberg Ph.D
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  4. Elias T. Zambidis M.D., Ph.D.
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  5. Albert D. Donnenberg Ph.D
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Editors and Affiliations

  1. Section of Surgery, Newport Specialty Hospital, Tustin, California, USA

    Melvin A. Shiffman

  2. Department of Plastic and Reconstructive Surgery, Marche Polytechnic University Medical School, University of Ancona, Ancona, Italy

    Alberto Di Giuseppe

  3. Department of Plastic Surgery, University of Padova, Padova, Italy

    Franco Bassetto

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Zimmerlin, L., Park, T.S., Donnenberg, V.S., Zambidis, E.T., Donnenberg, A.D. (2014). Pericytes: a Ubiquitous Source of Multipotent Adult Tissue Stem Cells. In: Shiffman, M., Di Giuseppe, A., Bassetto, F. (eds) Stem Cells in Aesthetic Procedures. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-45207-9_9

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