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Stem Cells, Mature Adipocytes, and Extracellular Matrix: What Does Each Contribute to Fat Graft Survival?

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Stem Cells in Aesthetic Procedures

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Abstract

For almost 100 years, fat tissue has been used as soft tissue filler for volume restoration. Fat grafting represents today a major tool in both reconstructive and aesthetic surgery. The discovery of adipose-derived stem cells (ASCs) opened new perspectives in basic science, experimental, and clinical plastic surgery. After fat grafting, the overlying skin gains in terms of thickness and quality. The authors have found that fat tissue can be used for two effects – volume restoration and tissue regeneration. Although the survival of adipose tissue has already been documented in different ways, the major drawback is the unpredictable resorption rates. For both clinical applications of the adipose tissue, an important issue to understand these processes and to solve the aforementioned. The authors discuss the role of cells, extracellular matrix, and biological factors in fat grafting which determine volumetric, regenerative, and bioengineering possibilities.

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References

  1. Sinna R, Delay E, Garson S, Mojallal A. Scientific bases of fat transfer. Critical review of the literature. Ann Chir Plast Esthet. 2006;51(3):223–30.

    Article  CAS  PubMed  Google Scholar 

  2. Brown SA, Levi B, Lequeux C, Wong VW, Mojallal A, Longaker MT. Basic science review on adipose tissue for clinicians. Plast Reconstr Surg. 2010;126(6): 1936–46.

    Article  CAS  PubMed  Google Scholar 

  3. Mojallal A, Foyatier JL. Historical review of the use of adipose tissue transfer in plastic and reconstructive surgery. Ann Chir Plast Esthet. 2004;49(5):419–25.

    Article  CAS  PubMed  Google Scholar 

  4. Mojallal A, Saint-Cyr M, Garrido I. Autologous fat transfer: controversies and current indications for breast surgery. J Plast Reconstr Aesthet Surg. 2009;62(5):708–10.

    Article  PubMed  Google Scholar 

  5. Auxenfans C, Lequeux C, Perrusel E, Mojallal A, Kinikoglu B, Damour O. Adipose-derived stem cells (ASCs) as a source of endothelial cells in the reconstruction of endothelialized skin equivalents. J Tissue Eng Regen Med. 2012;6(7):512–8.

    Article  CAS  PubMed  Google Scholar 

  6. Mitchell JB, McIntosh K, Zvonic S, Garrett S, Floyd ZE, Kloster A, Di Halvorsen Y, Storms RW, Goh B, Kilroy G, Wu X, Gimble JM. Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers. Stem Cells. 2006;24(2):376–85.

    Article  PubMed  Google Scholar 

  7. Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, Alfonso ZC, Fraser JK, Benhaim P, Hedrick MH. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 2002;13(12):4279–95.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  8. Mojallal A, Lequeux C, Shipkov C, Breton P, Foyatier JL, Braye F, Damour O. Improvement of skin quality after fat grafting: clinical observation and an animal study. Plast Reconstr Surg. 2009;124(3):765–74.

    Article  CAS  PubMed  Google Scholar 

  9. Sbarbati A, Galie M. The niche theory for fat graft survival. In: Coleman S, Mazzola R, editors. Fat injection from filling to regeneration. 1st ed. St. Louis: Quality Medical Publishing; 2009. p. 15–30.

    Google Scholar 

  10. Gir P, Oni G, Brown SA, Mojallal A, Rohrich RJ. Human adipose stem cells: current clinical applications11. Plast Reconstr Surg. 2012;129(6):1277–90.

    Article  CAS  PubMed  Google Scholar 

  11. Foyatier JL, Mojallal A, Voulliaume D, Comparin JP. Clinical evaluation of structural fat tissue graft (Lipostructure) in volumetric facial restoration with face-lift. About 100 cases. Ann Chir Plast Esthet. 2004;49(5):437–55.

    Article  PubMed  Google Scholar 

  12. Veber M, Tourasse C, Toussoun G, Moutran M, Mojallal A, Delay E. Radiographic findings after breast augmentation by autologous fat transfer. Plast Reconstr Surg. 2011;127(3):1289–99.

    Article  CAS  PubMed  Google Scholar 

  13. Veber M, Tourasse C, Moutran M, Mojallal A. Clinical analyses of clustered microcalcifications after autologous fat injection for breast augmentation. Plast Reconstr Surg. 2012;129(1):168e–9.

    Article  CAS  PubMed  Google Scholar 

  14. Mojallal A, Foyatier JL. The effect of different factors on the survival of transplanted adipocytes. Ann Chir Plast Esthet. 2004;49(5):426–36.

    Article  CAS  PubMed  Google Scholar 

  15. Mojallal A, Shipkov C, Braye F, Breton P, Foyatier JL. Influence of the recipient site on the outcomes of fat grafting in facial reconstructive surgery. Plast Reconstr Surg. 2009;124(2):471–83.

    Article  CAS  PubMed  Google Scholar 

  16. Peer LA. Cell survival theory versus replacement theory. Plast Reconstr Surg (1946). 1955;16(3):161–8.

    Article  CAS  Google Scholar 

  17. PeerR LA. The neglected free fat graft, its behavior and clinical use. Am J Surg. 1956;92(1):40–7.

    Article  Google Scholar 

  18. Lequeux C, Oni G, Mojallal A, Damour O, Brown SA. Adipose derived stem cells: efficiency, toxicity, stability of BrdU labeling and effects on self-renewal and adipose differentiation. Mol Cell Biochem. 2011;351(1–2):65–75.

    Article  CAS  PubMed  Google Scholar 

  19. Mojallal A, Auxenfans C, Lequeux C, Braye F, Damour O. Influence of negative pressure when harvesting adipose tissue on cell yield of the stromal-vascular fraction. Biomed Mater Eng. 2008;18(4–5):193–7.

    CAS  PubMed  Google Scholar 

  20. Mojallal A, Lequeux C, Shipkov C, Duclos A, Braye F, Rohrich R, Brown S, Damour O. Influence of age and body mass index on the yield and proliferation capacity of adipose-derived stem cells. Aesthetic Plast Surg. 2011;35(6):1097–105.

    Article  PubMed  Google Scholar 

  21. Yoshimura K, Matsumoto D, Gonda K. A clinical trial of soft tissue augmentation by lipoinjection with adipose-derived stromal cells (ASCs). In: Proceedings of the 3rd annual meeting of International Fat Applied Technology Society (IFATS), Charlottesville, 2005. pp 9–10.

    Google Scholar 

  22. Matsumoto D, Sato K, Gonda K, Takaki Y, Shigeura T, Sato T, Aiba-Kojima E, Iizuka F, Inoue K, Suga H, Yoshimura K. Cell-assisted lipotransfer: supportive use of human adipose-derived cells for soft tissue augmentation with lipoinjection. Tissue Eng. 2006;12(12):3375–82.

    Article  CAS  PubMed  Google Scholar 

  23. Lequeux C, Auxenfans C, Mojallal A, Sergent M, Damour O. Optimization of a culture medium for the differentiation of preadipocytes into adipocytes in a monolayer. Biomed Mater Eng. 2009;19(4–5):283–91.

    CAS  PubMed  Google Scholar 

  24. Mojallal A, Lequeux C, Auxenfans C, Braye F, Damour O. Does adipose tissue cultured with collagen matrix and preadipocytes give comparable results to the standard technique in plastic surgery? Biomed Mater Eng. 2008;18(4–5):187–92.

    CAS  PubMed  Google Scholar 

  25. Mojallal A, Lequeux C, Shipkov C, Rifkin L, Rohrich R, Duclos A, Brown S, Damour O. Stem cells, mature adipocytes, and extracellular scaffold: what does each contribute to fat graft survival? Aesthetic Plast Surg. 2011;35(6):1061–72.

    Article  PubMed  Google Scholar 

  26. Rigotti G, Marchi A, Galiè M, Baroni G, Benati D, Krampera M, Pasini A, Sbarbati A. Clinical treatment of radiotherapy tissue damage by lipoaspirate transplant: a healing process mediated by adipose-derived adult stem cells. Plast Reconstr Surg. 2007;119(5):1409–22.

    Article  CAS  PubMed  Google Scholar 

  27. Flynn LE, Prestwich GD, Semple JL, Woodhouse KA. Proliferation and differentiation of adipose-derived stem cells on naturally derived scaffolds. Biomaterials. 2008;29:1862–71.

    Article  CAS  PubMed  Google Scholar 

  28. Gimble JM, Katz AJ, Bunnell BA. Adipose-derived stem cells for regenerative medicine. Circ Res. 2007;100:1249–60.

    Article  CAS  PubMed  Google Scholar 

  29. Sterodimas A, de Faria J, Nicaretta B, Pitanguy I. Tissue engineering with adipose-derived stem cells (ADSCs): current and future applications. J Plast Reconstr Aesthet Surg. 2010;63:1886–92.

    Article  PubMed  Google Scholar 

  30. Coleman SR. Structural fat grafting: more than a permanent filler. Plast Reconstr Surg. 2006;118(3 Suppl):108S–20.

    Article  CAS  PubMed  Google Scholar 

  31. Sultan SM, Barr JS, Butala P, Davidson EH, Weinstein AL, Knobel D, Saadeh PB, Warren SM, Coleman SR, Hazen A. Fat grafting accelerates revascularisation and decreases fibrosis following thermal injury. J Plast Reconstr Aesthet Surg. 2012;65(2):219–27.

    Article  PubMed  Google Scholar 

  32. Garcia-Olmo D, Garcia-Arranz M, Herreros D. Expanded adipose-derived stem cells for the treatment of complex perianal fistula including Crohn’s disease. Expert Opin Biol Ther. 2008;8(9):1417–23.

    Article  CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Plastic, Reconstructive and Aesthetic Surgery, Croix Rousse Hospital, Hospices Civils De Lyon, University of Lyon, Lyon, France

    Ali Mojallal M.D., Ph.D. & Christo Shipkov M.D., Ph.D.

  2. Banque de Tissus et Cellules (Tissue and Cells Bank), Edouard Herriot Hospital, University of Lyon, Lyon, France

    Ali Mojallal M.D., Ph.D., Charlotte Lequeux Ph.D. & Odile Damour Ph.D.

  3. Department of Plastic Surgery, University of Texas Southwestern (UTSW), Medical Center, Dallas, TX, USA

    Ali Mojallal M.D., Ph.D., Lucas Rifkin M.D., Rod Rohrich M.D. & Spencer Brown Ph.D.

  4. University of Lyon, Lyon, France

    Ali Mojallal M.D., Ph.D.

  5. Research at the Stem Cell Lab, University of Pittsburgh, Pittsburgh, PA, USA

    Spencer Brown Ph.D.

Authors
  1. Ali Mojallal M.D., Ph.D.
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  2. Christo Shipkov M.D., Ph.D.
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  3. Charlotte Lequeux Ph.D.
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  4. Lucas Rifkin M.D.
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  5. Rod Rohrich M.D.
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  6. Spencer Brown Ph.D.
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  7. Odile Damour Ph.D.
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Corresponding author

Correspondence to Ali Mojallal M.D., Ph.D. .

Editor information

Editors and Affiliations

  1. Section of Surgery, Newport Specialty Hospital, Tustin, California, USA

    Melvin A. Shiffman

  2. Department of Plastic and Reconstructive Surgery, Marche Polytechnic University Medical School, University of Ancona, Ancona, Italy

    Alberto Di Giuseppe

  3. Department of Plastic Surgery, University of Padova, Padova, Italy

    Franco Bassetto

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Mojallal, A. et al. (2014). Stem Cells, Mature Adipocytes, and Extracellular Matrix: What Does Each Contribute to Fat Graft Survival?. In: Shiffman, M., Di Giuseppe, A., Bassetto, F. (eds) Stem Cells in Aesthetic Procedures. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-45207-9_11

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  • DOI: https://doi.org/10.1007/978-3-642-45207-9_11

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-45206-2

  • Online ISBN: 978-3-642-45207-9

  • eBook Packages: MedicineMedicine (R0)

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