Evaluating Study Designs to Investigate Dentin Hypersensitivity

  • Sahar TahaEmail author
  • Brian H. Clarkson


Studies for investigating dentin hypersensitivity are either epidemiological or testing for the effectiveness of a possible treatment. The latter can be tested in vitro or in vivo. The myriad number of studies evaluating possible treatments for the condition mandates the adoption of rigid criteria for the conduction of such studies in order to validate their results. Literature will be reviewed in this chapter to evaluate the different testing methods for the effectiveness of dentin hypersensitivity treatments.


Dentinal Tubule Smear Layer Dentin Surface Test Disc Dentin Hypersensitivity 
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  1. Abed AM, Mahdian M, Seifi M, Ziaei SA, Shamsaei M (2011) Comparative assessment of the sealing ability of Nd:YAG laser versus a new desensitizing agent in human dentinal tubules: a pilot study. Odontology 99(1):45–48. doi: 10.1007/s10266-010-0136-1 PubMedCrossRefGoogle Scholar
  2. Ahmed TR, Mordan NJ, Gilthorpe MS, Gillam DG (2005) In vitro quantification of changes in human dentine tubule parameters using SEM and digital analysis. J Oral Rehabil 32(8):589–597. doi: 10.1111/j.1365-2842.2005.01473.x, JOR1473 [pii]PubMedCrossRefGoogle Scholar
  3. Dijkman GE, Jongebloed WL, de Vries J, Ogaard B, Arends J (1994) Closing of dentinal tubules by glutaraldehyde treatment, a scanning electron microscopy study. Scand J Dent Res 102(3):144–150PubMedGoogle Scholar
  4. Earl JS, Leary RK, Muller KH, Langford RM, Greenspan DC (2011) Physical and chemical characterization of dentin surface following treatment with NovaMin technology. J Clin Dent 22(3):62–67PubMedGoogle Scholar
  5. Gillam DG, Khan N, Mordan NJ, Barber PM (1999) Scanning electron microscopy (SEM) investigation of selected desensitizing agents in the dentine disc model. Endod Dent Traumatol 15(5):198–204PubMedCrossRefGoogle Scholar
  6. Holland GR, Narhi MN, Addy M, Gangarosa L, Orchardson R (1997) Guidelines for the design and conduct of clinical trials on dentine hypersensitivity. J Clin Periodontol 24(11):808–813PubMedCrossRefGoogle Scholar
  7. Jain P, Vargas MA, Denehy GE, Boyer DB (1997) Dentin desensitizing agents: SEM and X-ray microanalysis assessment. Am J Dent 10(1):21–26PubMedGoogle Scholar
  8. Lynch E, Brauer DS, Karpukhina N, Gillam DG, Hill RG (2012) Multi-component bioactive glasses of varying fluoride content for treating dentin hypersensitivity. Dent Mater 28(2):168–178. doi: 10.1016/, S0109-5641(11)00922-5 [pii]PubMedCrossRefGoogle Scholar
  9. Mordan NJ, Barber PM, Gillam DG (1997) The dentine disc. A review of its applicability as a model for the in vitro testing of dentine hypersensitivity. J Oral Rehabil 24(2):148–156PubMedCrossRefGoogle Scholar
  10. Pereira JC, Segala AD, Gillam DG (2005) Effect of desensitizing agents on the hydraulic conductance of human dentin subjected to different surface pre-treatments – an in vitro study. Dent Mater 21(2):129–138. doi: 10.1016/, S0109564104000417 [pii]PubMedCrossRefGoogle Scholar
  11. Sauro S, Gandolfi MG, Prati C, Mongiorgi R (2006) Oxalate-containing phytocomplexes as dentine desensitisers: an in vitro study. Arch Oral Biol 51(8):655–664. doi: 10.1016/j.archoralbio.2006.02.010, S0003-9969(06)00038-0 [pii]PubMedCrossRefGoogle Scholar
  12. Suge T, Kawasaki A, Ishikawa K, Matsuo T, Ebisu S (2008) Ammonium hexafluorosilicate elicits calcium phosphate precipitation and shows continuous dentin tubule occlusion. Dent Mater 24(2):192–198. doi: 10.1016/, S0109-5641(07)00082-6 [pii]PubMedCrossRefGoogle Scholar
  13. Thanatvarakorn O, Nakashima S, Sadr A, Prasansuttiporn T, Ikeda M, Tagami J (2013) In vitro evaluation of dentinal hydraulic conductance and tubule sealing by a novel calcium-phosphate desensitizer. J Biomed Mater Res B Appl Biomater 101(2):303–309. doi: 10.1002/jbm.b.32840 PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Department of Conservative Dentistry, Faculty of DentistryUniversity of JordanAmmanJordan
  2. 2.Department of Cariology, Restorative Sciences and EndodonticsUniversity of Michigan School of DentistryAnn ArborUSA

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