Abstract
Tissue Engineering is defined as the technology aiming to apply the principles of engineering and life sciences towards the development of biological substitutes that restore, maintain, or improve tissue function or a whole organ. Its eventual goal is the creation of 3D artificial cell culture scaffolds that mimic the natural extracellular environment features sufficiently, so that cells function in the artificial medium as they would in vivo. Cells in tissue are surrounded by a dynamic cell type-dependent extracellular matrix that provides instructive cues at both the micro- and the nanoscale needed to maintain cell phenotype and behaviour. Cells are thus, inherently responsive to their environment, receptive to micro- and nanoscale features and patterns of chemistry and topography. Lasers are increasingly proving to be promising tools for the controlled and reproducible structuring of biomaterials at micro- and nanoscales. This chapter reviews current approaches for laser based fabrication of biomimetic tissue engineering scaffolds. These include laser processing of natural biomaterials synthesized to achieve certain compositions or properties similar to those of the extracellular matrix as well as novel laser fabrication technologies to achieve structural features on artificial materials mimicking the extracellular matrix morphology on various levels. The chapter concludes with the wealth of arising possibilities, demonstrating the excitement and significance of the laser based biomimetic materials processing for tissue engineering and regeneration.
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Skalak R, Fox C (1988) Tissue Engineering. In: Proceedings for a workshop held at granlibakken, Lake Tahoe, CA, February 26–29 New York, Alan Liss
Stevens MM, George JH (2005) Exploring and engineering the cell surface interface. Science 310(5751):1135–1138
Yu LMY, Leipzig ND, Shoichet MS (2008) Promoting neuron adhesion and growth. Mater Today 11(5):36–43
Seidlits SK, Lee JY, Schmidt CE (2008) Nanostructured scaffolds for neural applications. Nanomedicine-Uk 3(2):183–199
Hollister SJ (2005) Porous scaffold design for tissue Engineering. Nat Mater 4(7):518–524. doi:10.1038/nmat1421PII:nmat1421
Cretel E, Pierres A, Benoliel AM, Bongrand P (2008) How cells feel their environment: a focus on early dynamic events. Cell Mol Bioeng 1(1):5–14
Khang D, Lu J, Yao C, Haberstroh KM, Webster TJ (2008) The role of nanometer and sub-micron surface features on vascular and bone cell adhesion on titanium. Biomaterials 29(8):970–983
Gaspard S, Oujja M, Abrusci C, Catalina F, Lazare S, Desvergne JP, Castillejo M (2008) Laser induced foaming and chemical modifications of gelatine films. J Photoch Photobio A 193(2–3):187–192
Engelmayr GC, Cheng MY, Bettinger CJ, Borenstein JT, Langer R, Freed LE (2008) Accordion-like honeycombs for tissue engineering of cardiac anisotropy. Nat Mater 7(12):1003–1010
Chen GP, Ushida T, Tateishi T (2000) Hybrid biomaterials for tissue engineering: a preparative method for PLA or PLGA-collagen hybrid sponges. Adv Mater 12(6):455
Bruggeman JP, Bettinger CJ, Nijst CLE, Kohane DS, Langer R (2008) Biodegradable xylitol-based polymers. Adv Mater 20(10):1922
Wang YD, Ameer GA, Sheppard BJ, Langer R (2002) A tough biodegradable elastomer. Nat Biotechnol 20(6):602–606
Bettinger CJ (2009) Synthesis and microfabrication of biomaterials for soft-tissue engineering. Pure Appl Chem 81(12):2183–2201
Ifkovits JL, Burdick JA (2007) Review: photopolymerizable and degradable biomaterials for tissue engineering applications. Tissue Eng 13(10):2369–2385
Li HY, Du RL, Chang J (2005) Fabrication, characterization, and in vitro degradation of composite scaffolds based on PHBV and bioactive glass. J Biomater Appl 20(2):137–155
Martin AI, Salinas AJ, Vallet-Regi M (2005) Bioactive and degradable organic-inorganic hybrids. J Eur Ceram Soc 25(16):3533–3538
Sepulveda P, Jones JR, Hench LL (2002) Bioactive sol-gel foams for tissue repair. J Biomed Mater Res 59(2):340–348
Martin RA, Yue S, Hanna JV, Lee PD, Newport RJ, Smith ME, Jones JR (2012) Characterizing the hierarchical structures of bioactive sol-gel silicate glass and hybrid scaffolds for bone regeneration. Philos T R Soc A 370(1963):1422–1443
Valerio P, Guimaraes MHR, Pereira MM, Leite MF, Goes AM (2005) Primary osteoblast cell response to sol-gel derived bioactive glass foams. J Mater Sci-Mater M 16(9):851–856
Gough JE, Jones JR, Hench LL (2004) Nodule formation and mineralisation of human primary osteoblasts cultured on a porous bioactive glass scaffold. Biomaterials 25(11):2039–2046
Mano JF, Vaz CM, Mendes SC, Reis RL, Cunha AM (1999) Dynamic mechanical properties of hydroxyapatite-reinforced and porous starch-based degradable biomaterials. J Mater Sci-Mater M 10(12):857–862
Yang SF, Leong KF, Du ZH, Chua CK (2001) The design of scaffolds for use in tissue engineering. Part 1. Traditional factors. Tissue Eng 7(6):679–689
Lee J, Cuddihy MJ, Kotov NA (2008) Three-dimensional cell culture matrices: State of the art. Tissue Eng Pt B-Rev 14(1):61–86
Kurella A, Dahotre NB (2005) Review paper: surface modification for bioimplants: the role of laser surface engineering. J Biomater Appl 20(1):5–50. doi:10.1177/0885328205052974
Stratakis E, Ranella A, Fotakis C (2011) Biomimetic micro/nanostructured functional surfaces for microfluidic and tissue engineering applications. Biomicrofluidics 5(1):013411
Hutmacher DW, Sittinger M, Risbud MV (2004) Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems. Trends Biotechnol 22(7):354–362
Peltola SM, Melchels FPW, Grijpma DW, Kellomaki M (2008) A review of rapid prototyping techniques for tissue engineering purposes. Ann Med 40(4):268–280
Sachlos E, Czernuszka JT (2003) Making tissue Engineering scaffolds work. Review: the application of solid freeform fabrication technology to the production of tissue Engineering scaffolds. Eur Cell Mater 5:29–39; discussion 39–40. doi:vol005a03
Yeong WY, Chua CK, Leong KF, Chandrasekaran M (2004) Rapid prototyping in tissue engineering: challenges and potential. Trends Biotechnol 22(12):643–652
Schmidt M, Pohle D, Rechtenwald T (2007) Selective laser sintering of PEEK. Cirp Ann-Manuf Techn 56(1):205–208
Rimell JT, Marquis PM (2000) Selective laser sintering of ultra high molecular weight polyethylene for clinical applications. J Biomed Mater Res 53(4):414–420
Tan KH, Chua CK, Leong KF, Cheah CM, Cheang P, Abu Bakar MS, Cha SW (2003) Scaffold development using selective laser sintering of polyetheretherketone-hydroxyapatite biocomposite blends. Biomaterials 24(18):3115–3123
Williams JM, Adewunmi A, Schek RM, Flanagan CL, Krebsbach PH, Feinberg SE, Hollister SJ, Das S (2005) Bone tissue engineering using polycaprolactone scaffolds fabricated via selective laser sintering. Biomaterials 26(23):4817–4827
Kanczler JM, Mirmalek-Sani SH, Hanley NA, Ivanov AL, Barry JJA, Upton C, Shakesheff KM, Howdle SM, Antonov EN, Bagratashvili VN, Popov VK, Oreffo ROC (2009) Biocompatibility and osteogenic potential of human fetal femur-derived cells on surface selective laser sintered scaffolds. Acta Biomater 5(6):2063–2071
Zhou WY, Lee SH, Wang M, Cheung WL, Ip WY (2008) Selective laser sintering of porous tissue engineering scaffolds from poly(L)/carbonated hydroxyapatite nanocomposite microspheres. J Mater Sci-Mater M 19(7):2535–2540
Wiria FE, Leong KF, Chua CK, Liu Y (2007) Poly-epsilon-caprolactone/hydroxyapatite for tissue engineering scaffold fabrication via selective laser sintering. Acta Biomater 3(1):1–12
Simpson RL, Wiria FE, Amis AA, Chua CK, Leong KF, Hansen UN, Chandraselkaran M, Lee MW (2008) Development of a 95/5 poly(L-lactide-co-glycolide)/hydroxylapatite and beta-tricalcium phosphate scaffold as bone replacement material via selective laser sintering. J Biomed Mater Res B 84B(1):17–25
Duan B, Wang M, Zhou WY, Cheung WL, Li ZY, Lu WW (2010) Three-dimensional nanocomposite scaffolds fabricated via selective laser sintering for bone tissue engineering. Acta Biomater 6(12):4495–4505
Levy RA, Chu TMG, Halloran JW, Feinberg SE, Hollister S (1997) CT-generated porous hydroxyapatite orbital floor prosthesis as a prototype bioimplant. Am J Neuroradiol 18(8):1522–1525
Griffith ML, Halloran JW (1996) Freeform fabrication of ceramics via stereolithography. J Am Ceram Soc 79(10):2601–2608
Matsuda T, Mizutani M (2002) Liquid acrylate-endcapped biodegradable poly(epsilon-caprolactone-co-trimethylene carbonate). II. Computer-aided stereolithographic microarchitectural surface photoconstructs. J Biomed Mater Res 62(3):395–403
Cooke MN, Fisher JP, Dean D, Rimnac C, Mikos AG (2003) Use of stereolithography to manufacture critical-sized 3D biodegradable scaffolds for bone ingrowth. J Biomed Mater Res B 64B(2):65–69
Dhariwala B, Hunt E, Boland T (2004) Rapid prototyping of tissue-engineering constructs, using photopolymerizable hydrogels and stereolithography. Tissue Eng 10(9–10):1316–1322
Melchels FPW, Feijen J, Grijpma DW (2009) A poly(D, L-lactide) resin for the preparation of tissue engineering scaffolds by stereolithography. Biomaterials 30(23–24):3801–3809
Lee JW, Nguyen TA, Kang KS, Seol YJ, Cho DW (2008) Development of a growth factor-embedded scaffold with controllable pore size and distribution using micro-stereolithography. Tissue Eng Pt A 14(5):835–835
Arcaute K, Mann BK, Wicker RB (2006) Stereolithography of three-dimensional bioactive poly(ethylene glycol) constructs with encapsulated cells. Ann Biomed Eng 34(9):1429–1441
Cumpston BH, Ananthavel SP, Barlow S, Dyer DL, Ehrlich JE, Erskine LL, Heikal AA, Kuebler SM, Lee IYS, McCord-Maughon D, Qin JQ, Rockel H, Rumi M, Wu XL, Marder SR, Perry JW (1999) Two-photon polymerization initiators for three-dimensional optical data storage and microfabrication. Nature 398(6722):51–54
Farsari M, Chichkov BN (2009) Two-photon fabrication. Nat Photonics 3(8):450–452
Almany L, Seliktar D (2005) Biosynthetic hydrogel scaffolds made from fibrinogen and polyethylene glycol for 3D cell cultures. Biomaterials 26(15):2467–2477
Hsieh TM, Ng CWB, Narayanan K, Wan ACA, Ying JY (2010) Three-dimensional microstructured tissue scaffolds fabricated by two-photon laser scanning photolithography. Biomaterials 31(30):7648–7652
Ovsianikov A, Schlie S, Ngezahayo A, Haverich A, Chichkov BN (2007) Two-photon polymerization technique for microfabrication of CAD-designed 3D scaffolds from commercially available photosensitive materials. J Tissue Eng Regen M 1(6):443–449
Livage J, Sanchez C (1992) sol-gel chemistry. J Non-Cryst Solids 145(1–3):11–19
Ovsianikov A, Viertl J, Chichkov B, Oubaha M, MacCraith B, Sakellari I, Giakoumaki A, Gray D, Vamvakaki M, Farsari M, Fotakis C (2008) Ultra-low shrinkage hybrid photosensitive material for two-photon polymerization microfabrication. Acs Nano 2(11):2257–2262
Sakellari I, Gaidukeviciute A, Giakoumaki A, Gray D, Fotakis C, Farsari M, Vamvakaki M, Reinhardt C, Ovsianikov A, Chichkov BN (2010) Two-photon polymerization of titanium-containing sol-gel composites for three-dimensional structure fabrication. Appl Phys a-Mater 100(2):359–364
Psycharakis S, Tosca A, Melissinaki V, Giakoumaki A, Ranella A (2011) Tailor-made three-dimensional hybrid scaffolds for cell cultures. Biomed Mater 6(4):045008
Claeyssens F, Hasan EA, Gaidukeviciute A, Achilleos DS, Ranella A, Reinhardt C, Ovsianikov A, Xiao S, Fotakis C, Vamvakaki M, Chichkov BN, Farsari M (2009) Three-dimensional biodegradable structures fabricated by two-photon polymerization. Langmuir 25(5):3219–3223
Mizutani M, Matsuda T (2002) Liquid photocurable biodegradable copolymers: In vivo degradation of photocured poly(epsilon-caprolactone-co-trimethylene carbonate). J Biomed Mater Res 61(1):53–60
Mizutani M, Matsuda T (2002) Photocurable liquid biodegradable copolymers: in vitro hydrolytic degradation behaviors of photocured films of coumarin-endcapped poly(epsilon-caprolactone-co-trimethylene carbonate). Biomacromolecules 3(2):249–255
Melissinaki V, Gill AA, Ortega I, Vamvakaki M, Ranella A, Haycock JW, Fotakis C, Farsari M, Claeyssens F (2011) Direct laser writing of 3D scaffolds for neural tissue engineering applications. Biofabrication 3(4):045005
Ke K, Hasselbrink EF, Hunt AJ (2005) Rapidly prototyped three-dimensional nanofluidic channel networks in glass substrates. Anal Chem 77(16):5083–5088
Zorba V, Stratakis E, Barberoglou M, Spanakis E, Tzanetakis P, Anastasiadis SH, Fotakis C (2008) Biomimetic artificial surfaces quantitatively reproduce the water repellency of a lotus leaf. Adv Mater 20(21):4049
Ranella A, Barberoglou M, Bakogianni S, Fotakis C, Stratakis E (2010) Tuning cell adhesion by controlling the roughness and wettability of 3D micro/nano silicon structures. Acta Biomater 6(7):2711–2720
Doraiswamy A, Patz T, Narayan RJ, Dinescu M, Modi R, Auyeung RCY, Chrisey DB (2006) Two-dimensional differential adherence of neuroblasts in laser micromachined CAD/CAM agarose channels. Appl Surf Sci 252(13):4748–4753
Duncan AC, Rouais F, Lazare S, Bordenave L, Baquey C (2007) Effect of laser modified surface microtopochemistry on endothelial cell growth. Colloid Surf B 54(2):150–159
Miller PR, Aggarwal R, Doraiswamy A, Lin YJ, Lee YS, Narayan RJ (2009) Laser micromachining for biomedical applications. Jom-Us 61(9):35–40
Patz TM, Doraiswamy A, Narayan RJ, Modi R, Chrisey DB (2005) Two-dimensional differential adherence and alignment of C2C12 myoblasts. Mat Sci Eng B-Solid 123(3):242–247
Choi HW, Johnson JK, Nam J, Farson DF, Lannutti J (2007) Structuring electrospun polycaprolactone nanofiber tissue scaffolds by femtosecond laser ablation. J Laser Appl 19(4):225–231
Papadopoulou EL, Samara A, Barberoglou M, Manousaki A, Pagakis SN, Anastasiadou E, Fotakis C, Stratakis E (2010) Silicon scaffolds promoting three-dimensional neuronal Web of cytoplasmic processes. Tissue Eng Part C-Me 16(3):497–502
Masoumi KL, Johnson JT, Zugates GC, Engelmayr GC (2011) IEEE 37th annual northeast bioengineering conference (NEBEC), 1–2, April 2011
Wüst S, Müller R, Hofmann S (2011) Controlled positioning of cells in biomaterials-approaches towards 3D tissue printing. J Funct Biomater 2(3):119–154
Guillotin B, Souquet A, Catros S, Duocastella M, Pippenger B, Bellance S, Bareille R, Remy M, Bordenave L, Amedee J, Guillemot F (2010) Laser assisted bioprinting of engineered tissue with high cell density and microscale organization. Biomaterials 31(28):7250–7256
Mironov V, Boland T, Trusk T, Forgacs G, Markwald RR (2003) Organ printing: computer-aided jet-based 3D tissue engineering. Trends Biotechnol 21(4):157–161
Schiele NR, Corr DT, Huang Y, Raof NA, Xie YB, Chrisey DB (2010) Laser-based direct-write techniques for cell printing. Biofabrication 2(3):032001
Ovsianikov A, Gruene M, Pflaum M, Koch L, Maiorana F, Wilhelmi M, Haverich A, Chichkov B (2010) Laser printing of cells into 3D scaffolds. Biofabrication 2(1):014104
Gittard SD, Narayan R (2010) Laser direct writing of micro- and nano-scale medical devices. Expert Rev Med Devic 7(3):343–356
Chu TMG, Halloran JW, Hollister SJ, Feinberg SE (2001) Hydroxyapatite implants with designed internal architecture. J Mater Sci-Mater M 12(6):471–478
Koufaki N, Ranella A, Aifantis KE, Barberoglou M, Psycharakis S, Fotakis C, Stratakis E (2011) Controlling cell adhesion via replication of laser micro/nano-textured surfaces on polymers. Biofabrication 3(4):045004
Willerth SM, Sakiyama-Elbert SE (2008) Combining stem cells and biomaterial scaffolds for constructing tissues and cell delivery. doi:NBK27050[bookaccession]
Aguilar CA, Lu Y, Mao S, Chen SC (2005) Direct micro-patterning of biodegradable polymers using ultraviolet and femtosecond lasers. Biomaterials 26(36):7642–7649
Narayan RJ, Jin CM, Patz T, Doraiswamy A, Modi R, Chrisey DB, Su YY, Lin SJ, Ovsianikov A, Chichkov B (2005) Laser processing of advanced biomaterials. Adv Mater Process 163(4):39–42
Vogel A, Noack J, Huttman G, Paltauf G (2005) Mechanisms of femtosecond laser nanosurgery of cells and tissues. Appl Phys B-Lasers O 81(8):1015–1047
Ritschdorff ET, Shear JB (2010) Multiphoton lithography using a high-repetition rate microchip laser. Anal Chem 82(20):8733–8737
Seidlits SK, Schmidt CE, Shear JB (2009) High-resolution patterning of hydrogels in three dimensions using direct-write photofabrication for cell guidance. Adv Funct Mater 19(22):3543–3551
Turunen S, Kapyla E, Terzaki K, Viitanen J, Fotakis C, Kellomaki M, Farsari M (2011) Pico- and femtosecond laser-induced crosslinking of protein microstructures: evaluation of processability and bioactivity. Biofabrication 3(4):045002
Ovsianikov A, Deiwick A, Van Vlierberghe S, Dubruel P, Moller L, Drager G, Chichkov B (2011) Laser fabrication of three-dimensional CAD scaffolds from photosensitive gelatin for applications in tissue Engineering. Biomacromolecules 12(4):851–858
Ovsianikov A, Deiwick A, Van Vlierberghe S, Pflaum M, Wilhelmi M, Dubruel P, Chichkov B (2011) Laser fabrication of 3D gelatin scaffolds for the generation of bioartificial tissues. Materials 4(1):288–299
Lazare S, Tokarev V, Sionkowska A, Wisniewski M (2005) Surface foaming of collagen, chitosan and other biopolymer films by KrF excimer laser ablation in the photomechanical regime. Appl Phys a-Mater 81(3):465–470
Wisniewski M, Sionkowska A, Kaczmarek H, Lazare S, Tokarev V (2007) Influence of laser irradiation on the thin collagen films. part I. mechanism of micro-foam structure formation and collagen surface ablation. Polimery-W 52(4):259–267
Gaspard S, Oujja M, de Nalda R, Abrusci C, Catalina F, Banares L, Lazare S, Castillejo M (2007) Nanofoaming in the surface of biopolymers by femtosecond pulsed laser irradiation. Appl Surf Sci 254(4):1179–1184
Sionkowska A, Kaczmarek H, Wisniewski M, Skopinska J, Lazare S, Tokarev V (2006) The influence of UV irradiation on the surface of chitosan films. Surf Sci 600(18):3775–3779
Gaspard S, Oujja A, de Nalda R, Abrusci C, Catalina F, Banares L, Castillejo M (2007) Submicron foaming in gelatine by nanosecond and femtosecond pulsed laser irradiation. Appl Surf Sci 253(15):6420–6424
Gaspard S, Oujja M, de Nalda R, Castillejo M, Banares L, Lazare S, Bonneau R (2008) Nanofoaming dynamics in biopolymers by femtosecond laser irradiation. Appl Phys a-Mater 93(1):209–213
Lazare S, Bonneau R, Gaspard S, Oujja M, De Nalda R, Castillejo M, Sionkowska A (2009) Modeling the dynamics of one laser pulse surface nanofoaming of biopolymers. Appl Phys a-Mater 94(4):719–729
Wisniewski M, Sionkowska A, Kaczmarek H, Skopinska J, Lazare S, Tokarev V (2006) The influence of KrF excimer laser irradiation on the surface of collagen and collagen/PVP films. Int J Photoenergy
Klini A, Loukakos PA, Gray D, Manousaki A, Fotakis C (2008) Laser induced forward transfer of metals by temporally shaped femtosecond laser pulses. Opt Expr 16(15):11300–11309. doi:167754[pii]
Tzortzakis S, Papazoglou DG, Zergioti I (2006) Long-range filamentary propagation of subpicosecond ultraviolet laser pulses in fused silica. Opt Lett 31(6):796–798
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Stratakis, E., Ranella, A., Fotakis, C. (2013). Laser-Based Biomimetic Tissue Engineering. In: Schmidt, V., Belegratis, M. (eds) Laser Technology in Biomimetics. Biological and Medical Physics, Biomedical Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-41341-4_9
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