Segmentation of Time-Lapse Images with Focus on Microscopic Images of Cells

  • Jindřich Soukup
  • Petr Císař
  • Filip Šroubek
Part of the Lecture Notes in Computer Science book series (LNCS, volume 8157)


Phase contrast is a noninvasive microscopy imaging technique that is widely used in time-lapse imaging of cells. Resulting images however contain some optical artifacts, which makes automated processing by computer difficult.

We developed a novel algorithm for cell segmentation. It is based on processing of time differences between images and combination of thresholding, blurring and morphological operations. We tested the algorithm on four different cell types acquired by two different microscopes. We evaluated the precision of segmentation against the manual segmentation by human operator and compared also with other methods. Our algorithm is simple, fast and shows accuracy that is comparable to manual segmentation. In addition it can correctly separate the dead from living cells.


cell segmentation phase-contrast microscopy 


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Jindřich Soukup
    • 1
    • 2
    • 3
  • Petr Císař
    • 3
  • Filip Šroubek
    • 2
  1. 1.Charles University in PraguePrague 1Czech Republic
  2. 2.Institute of Information Theory and Automation of the ASCRPrague 8Czech Republic
  3. 3.FFPW, CENAKVAUniversity of South Bohemia in České BudějoviceČeské BudějoviceCzech Republic

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