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Artemisia annua - Pharmacology and Biotechnology pp 125–137Cite as

Potential Methods to Improve the Efficiency of Artemisinin Extraction from Artemisia annua

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Abstract

Recently, there has been a move to improve the extraction of artemisinin in terms of energy, solvent and purification costs and so, as a result, many innovative processes, such as supercritical fluid extraction (SFE), pressurised solvent extraction (PSE), microwave-assisted extraction (MAE) and ultrasonic-assisted extraction (UAE), are currently being explored. The most basic industrial-scale method for extracting artemisinin involves high volumes of heated organic solvents, such as petroleum ether and hexane, coupled with extended extraction time periods in order to achieve reasonable quantities of extract. Solvent considerations are not only underpinned by the solubility of artemisinin but also that of other phytological compounds which can complicate the purification stages. This has lead to some of the obvious alternative solvent choices, such as ethanol, being considered as unviable. Therefore, some of the more modern technologies have largely maintained the use of the traditional solvents but altered the parameters with which they are used, such as increasing pressures and temperatures of reactants in order to speed up extraction. This chapter will include an evaluation of the aforementioned alternate extraction techniques (SFE, PSE, MAE and UAE), including consideration of artemisinin yield, extract purity and industrial feasibility, in addition to examining the advantages and disadvantages of a range of solvent polarities.

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References

  • Ai Z, Li J, Zhang L, Lee S (2010) Rapid decolorization of azo dyes in aqueous solution by an ultrasound-assisted electrocatalytic oxidation process. Ultrasonics Sonochem 17:370–375

    Article  CAS  Google Scholar 

  • Albu S, Joyce E, Paniwnyk L, Lorimer J, Mason T (2004) Potential for the use of ultrasound in the extraction of antioxidants from Rosmarinus officinalis for the food and pharmaceutical industry. Ultrason Sonochem 11:261–265

    Article  PubMed  CAS  Google Scholar 

  • Asakura Y, Nishida T, Matsuoka T, Koda S (2008) Effects of ultrasonic frequency and liquid height on sonochemical efficiency of large-scale sonochemical reactors. Ultrason Sonochem 15:244–250

    Article  PubMed  CAS  Google Scholar 

  • Asfaw N, Licence P, Novitskii AA, Poliakoff M (2005) Green chemistry in Ethiopia: The cleaner extraction of essential oils from Artemisia afra. A comparison of clean technology with conventional methodology. Green Chem 7(5):352–356

    Article  CAS  Google Scholar 

  • Birkin P, Leighton T, Watson Y, Power J (2001) Acoustoelectrochemistry. Acoustics Bulletin 26(5):23–37

    Google Scholar 

  • Briars R, Paniwnyk L (2013) Effect of ultrasound on the extraction of artemisinin from Artemisia annua. Ind Crops Prod 42:595–600

    Article  CAS  Google Scholar 

  • Chemat F, Vian MA, Cravotto G (2012) Green extraction of natural products: concept and principles. Int J Mol Sci 13(7):8615–8627

    Article  PubMed  CAS  Google Scholar 

  • Christen P, Veuthey J (2001) New trends in extraction, identification and quantification of artemisinin and its derivatives. Curr Med Chem 8:1827–1839

    Article  PubMed  CAS  Google Scholar 

  • Corr S (2002) 1,1,1,2-tetrafluoroethane; from refrigerant and propellant to solvent. J Fluorine Chem 118(1–2):55–67

    Article  CAS  Google Scholar 

  • Cutler M, Lapkin A (2007) Separation of artemisinin from primary extracts: a study commissioned through medicines for malaria venture (MMV) [online]. Available from <http://www.mmv.org/newsroom/publications/separation-artemisinin-primary-extracts>[2012]

  • Cutler M, Lapkin A, Plucinski P (2006) Comparative assessment of technologies for extraction of artemisinin: a study commissioned through medicines for malaria venture (MMV) [online] [2012]

    Google Scholar 

  • Dahlem O, Reisse J, Halloin V (1999) The radially vibrating horn: a scaling-up possibility for sonochemical reactions. Chem Eng Sci 54:2829–2838

    Article  CAS  Google Scholar 

  • Elsohly HN, Croom EM Jr, El-Feraly FS, El-Sherei MM (1990) A large-scale extraction technique of artemisinin from Artemisia annua. J Nat Prod (Lloydia) 53(6):1560–1564

    Article  CAS  Google Scholar 

  • Glisic S, Ristic M, Skala D (2011) The combined extraction of sage (Salvia Officinalis L.): ultrasound followed by supercritical CO2 extraction. Ultrason Sonochem 18:318–326

    Article  PubMed  CAS  Google Scholar 

  • Gogate P, Pandit A (2004) Sonochemical reactors: scale up aspects. Ultrason Sonochem 11:105–117

    Article  PubMed  CAS  Google Scholar 

  • Grevenstuk T, Gonçalves S, Nogueira JMF, Bernardo-Gil MG, Romano A (2012) Recovery of high purity plumbagin from Drosera intermedia. Ind Crops Prod 35(1):257–260

    Article  CAS  Google Scholar 

  • Guo Z, Jin Q, Fan G, Duan Y, Qin C, Wen M (2001) Microwave-assisted extraction of effective constituents from a chinese herbal medicine radix puerariae. Anal Chim Acta 436(1):41–47

    Article  CAS  Google Scholar 

  • Han Q (ed) (2007) Materials processing under the influence of external fields. The use of power ultrasound for material processing: The minerals, metals and materials society (TMS)

    Google Scholar 

  • Haynes RK, Vonwiller SC (1994) Extraction of artemisinin and artemisinic acid: preparation of artemether and new analogues. Trans R Soc Trop Med Hyg 88(Suppl. 1):S23–S26

    Article  PubMed  Google Scholar 

  • Jadhav D, Parag R, Gogate PR, Rathod VK (2009) Extraction of vanillin from Vanilla pods: a comparison study of conventional soxhlet and ultrasound assisted extraction. J Food Eng 93(4):421–426

    Google Scholar 

  • Keil F, Dahnke S (1997) Numerical calculation of scale-up effects of pressure fields in sonochemical reactors-homogeneous phase. Hung J Ind Chem 25:71–80

    CAS  Google Scholar 

  • Khalil M, Darusman LK, Syafitri UD (2011) Application of fractional factorial design to optimize extraction method of artemisinin from Artemisia annua. ScienceAsia 37(3):219–224

    Article  CAS  Google Scholar 

  • Kohler M, Haerdi W, Christen P, Veuthey J (1997) Extraction of artemisinin and artemisinic acid from Artemisia annua L. using supercritical carbon dioxide. J Chromatogr A 785:353–360

    Article  PubMed  CAS  Google Scholar 

  • Kuhn T, Wang Y (2007) Artemisinin—an Innovative cornerstone for anti-malaria therapy. Prog Drug Res 65:385–422

    Google Scholar 

  • Kumar A, Gogate P, Pandit A (2007) Mapping the efficacy of new designs for large scale sonochemical reactors. Ultrason Sonochem 14:538–544

    Article  PubMed  CAS  Google Scholar 

  • Lapkin A, Plucinski P, Cutler M (2006) Comparative assessment of technologies for extraction of artemisinin. J Nat Prod 69:1653–1664

    Article  PubMed  CAS  Google Scholar 

  • Lapkin AA, Peters M, Greiner L, Chemat S, Leonhard K, Liauw MA, Leitner W (2010) Screening of new solvents for artemisinin extraction process using ab initio methodology. Green Chem 12(2):241–251

    Article  CAS  Google Scholar 

  • Leighton T (2007) What is ultrasound? Prog Biophys Mol Biol 93:3–83

    Article  PubMed  Google Scholar 

  • Leonelli C, Mason T (2010) Microwave and ultrasonic processing: now a realistic option for industry. Chem Eng Process 49:885–900

    Article  CAS  Google Scholar 

  • Li Y, Skouroumounis GK, Elsey GM, Taylor DK (2011) Microwave-assistance provides very rapid and efficient extraction of grape seed polyphenols. Food Chem 129(2):570–576

    Article  CAS  Google Scholar 

  • Lin Y, Yang C, Hsu H, Hsu S, Chang CJ (2006) Response surface methodology to supercritical fluids extraction of artemisinin and the effects on rat hepatic stellate cell in vitro. J Supercrit Fluids 39(1):48–53

    Article  CAS  Google Scholar 

  • Liu S, Tian N, Liu Z, Huang J, Li J, Ferreira J (2008) Affordable and sensitive determination of artemisinin in Artemisia annua L. by gas chromatography with electron-capture detection. J Chromatogr A 1190:302–306

    Article  PubMed  CAS  Google Scholar 

  • Liu X, Ren N, Ma F (2007) Effect of powdered activated carbon on chinese traditional medicine wastewater treatment in submerged membrane bioreactor with electronic control backwashing. J Environ Sci 19(9):1037–1042

    Article  CAS  Google Scholar 

  • Liu NQ, Schuehly W, von Freyhold M, Van der Kooy F (2011) A novel purification method of artemisinin from Artemisia annua. Ind Crops Prod 34(1):1084–1088

    Article  Google Scholar 

  • Liu Y, Lü H, Pang F (2009) Solubility of artemisinin in seven different pure solvents from (283.15–323.15) K. J Chem Eng Data 54(3):762–764

    Article  CAS  Google Scholar 

  • Martín L, Mainar AM, González-Coloma A, Burillo J, Urieta JS (2011) Supercritical fluid extraction of wormwood (Artemisia absinthium L.). J Supercrit Fluids 56(1):64–71

    Article  Google Scholar 

  • Mason T, Cobley A, Graves J, Morgan D (2011) New evidence for the inverse dependence of mechanical and chemical effects on the frequency of ultrasound. Ultrason Sonochem 18:226–230

    Article  PubMed  CAS  Google Scholar 

  • Mason T (2007) Developments in ultrasound—non-medical. Prog Biophys Mol Biol 93:166–175

    Article  PubMed  Google Scholar 

  • Mason T (1999) Sonochemistry: current uses and future prospects in the chemical and processing industries. Philos Trans R Soc A 357:355–369

    Article  CAS  Google Scholar 

  • Miron T, Plaza M, Bahrim G, Ibanez E, Herrero M (2010) Chemical composition of bioactive pressurized extracts of Romanian aromatic plants. J Chromatogr A 1218:4918

    PubMed  Google Scholar 

  • Neill US (2011) From branch to bedside: Youyou Tu is awarded the 2011 Lasker–DeBakey Clinical Medical Research Award for discovering artemisinin as a treatment for malaria. J Clin Inv 121(10):2768–3773

    Article  Google Scholar 

  • Nti-Gyabaah J, Gbewonyo K, Chiew YC (2010) Solubility of artemisinin in different single and binary solvent mixtures between (284.15 and 323.15) K and NRTL interaction parameters. J Chem Eng Data 55(9):3356–3363

    Article  CAS  Google Scholar 

  • Ormeño E, Goldstein A, Niinemets T (2011) Extracting and trapping biogenic volatile organic compounds stored in plant species. TrAC—Trends Anal Chem 30(7):978–989

    Article  Google Scholar 

  • Paniwnyk L, Cai H, Albu S, Mason T, Cole R (2009) The enhancement and scale up of the extraction of anti-oxidants from Rosmarinus officinalis using ultrasound. Ultrason Sonochem 16:287–292

    Article  PubMed  CAS  Google Scholar 

  • Pourmortazavi SM, Hajimirsadeghi SS (2007) Supercritical fluid extraction in plant essential and volatile oil analysis. J Chromatogr A 1163(1–2):2–24

    PubMed  CAS  Google Scholar 

  • Quispe-Condori S, Sánchez D, Foglio MA, Rosa PTV, Zetzl C, Brunner G, Meireles MAA (2005) Global yield isotherms and kinetic of artemisinin extraction from Artemisia annua L leaves using supercritical CO2. J Supercrit Fluids 36(1):40–48

    Article  CAS  Google Scholar 

  • Schilmiller A, Last R, Pichersky E (2008) Harnessing plant trichome biochemistry for the production of useful compounds. Plant J 54:702–711

    Article  PubMed  CAS  Google Scholar 

  • Sticher O (2008) Natural product isolation. Nat Prod Rep 25(3):517–554

    Article  PubMed  CAS  Google Scholar 

  • Stringham R, Lynam K, Mrozinski P, Kilby G, Pelczer I, Kraml C (2009) High Performance liquid chromatographic evaluation of artemisinin, raw material in the synthesis of artesunate and artemether. J Chromatogr A 1216:8918–8925

    Article  PubMed  CAS  Google Scholar 

  • Sun Y, Liu D, Chen J, Ye X, Yu D (2011) Effects of different factors of ultrasound treatment on the extraction yield of the all-Trans-β-carotene from citrus peels. Ultrason Sonochem 18:243–249

    Article  PubMed  CAS  Google Scholar 

  • Sutkar V, Gogate P, Csoka L (2010) Theoretical prediction of cavitational activity distribution in sonochemical reactors. Chem Eng J 158:290–295

    Article  CAS  Google Scholar 

  • Tang F, Zhang Q, Nie Z, Chen B, Yao S (2009) Sample preparation for analyzing traditional Chinese medicines. Trends Anal Chem 28(11):1253–1262

    Article  CAS  Google Scholar 

  • Tzeng T, Lin Y, Jong T, Chang CJ (2007) Ethanol modified supercritical fluids extraction of scopoletin and artemisinin from Artemisia annua L. Sep Purif Technol 56(1):18–24

    Article  CAS  Google Scholar 

  • Veillet S, Tomao V, Chemat F (2010) Ultrasound assisted maceration: an original procedure for direct aromatisation of olive oil with basil. Food Chem 123:905–911

    Article  CAS  Google Scholar 

  • Wang B, Deng C (eds) (2006) The design optimization of ultrasonic equipment for Chinese traditional medicine extraction. In: IEEE international conference on mechatronics and automation, 25–28 June, Luoyang, China

    Google Scholar 

  • Wang C, Chou Y, Sheu S, Jang M, Chen T (2011) Application of ultrasound thermal process on extracting flavor and caffeine of coffee. Therm Sci 15(Suppl.):S69–S74

    Google Scholar 

  • Xia E, Ai X, Zang S, Guan T, Xu X, Li H (2011) Ultrasound-assisted extraction of phillyrin from Forsythia suspensa. Ultrason Sonochem 18:549–552

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Coventry University, Faculty of Health and Life Sciences, Priory Street, Coventry, CV1 5FB, UK

    Rhianna Briars & Larysa Paniwnyk

Authors
  1. Rhianna Briars
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  2. Larysa Paniwnyk
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Correspondence to Rhianna Briars .

Editor information

Editors and Affiliations

  1. Department of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany

    Tariq Aftab

  2. US Salinity Laboratory, United States Department of Agriculture Agriculture Research Service, Riverside, California, USA

    Jorge F.S. Ferreira

  3. Botany Department, Aligarh Muslim University, Aligarh, India

    M. Masroor A. Khan

  4. Botany Department, Aligarh Muslim University, Aligarh, India

    M. Naeem

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Briars, R., Paniwnyk, L. (2014). Potential Methods to Improve the Efficiency of Artemisinin Extraction from Artemisia annua . In: Aftab, T., Ferreira, J., Khan, M., Naeem, M. (eds) Artemisia annua - Pharmacology and Biotechnology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-41027-7_8

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