Abstract
CPEs have established themselves as useful imaging agents for live cells due to their superior properties of large extinction coefficients, good photostability, large two-photon action cross sections, low cytotoxicity, and versatile surface modification. In this chapter, fluorescence imaging applications of functionalized conjugated polyelectrolytes are presented, where specific cells imaging have been successfully achieved by functionalizing CPEs with specific recognition elements to attain selective binding. Specific cellular organelle imaging has also been successfully achieved with CPEs. As a new water-dispersible form of conjugated polymers, conjugated polymer nanoparticles present great potential in biological imaging due to their superior properties, which are comprehensively introduced in this chapter.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Celli JP, Spring BQ, Rizvi I, Evans CL, Samkoe KS, Verma S, Pogue BW, Hasan T (2010) Imaging and photodynamic therapy: mechanisms, monitoring, and optimization. Chem Rev 110:2795–2838
McRae RL, Phillips RL, Kim I-B, Bunz UHF, Fahrni CJ (2008) Molecular recognition based on low-affinity polyvalent interactions: selective binding of a carboxylated polymer to fibronectin fibrils of live fibroblast cells. J Am Chem Soc 130:7851–7853
Zhu C, Yang Q, Liu L, Wang S (2011) A potent fluorescent probe for the detection of cell apoptosis. Chem Commun 47:5524–5526
Pecher J, Mecking S (2010) Nanoparticles of conjugated polymers. Chem Rev 110:6260–6279
Moon JH, McDaniel W, MacLean P, Hancock LE (2007) Live-cell-permeable poly (p-phenylene ethynylene). Angew Chem Int Ed 46:8223–8225
Rahim NAA, McDaniel W, Bardon K, Srinivasan S, Vickerman V, So PTC, Moon JH (2009) Conjugated polymer nanoparticles for two-photon imaging of endothelial cells in a tissue model. Adv Mater 21:3492–3496
Howes P, Green M, Levitt J, Suhling K, Hughes M (2010) Phospholipid encapsulated semiconducting polymer nanoparticles: their use in cell imaging and protein attachment. J Am Chem Soc 132:3989–3996
Pu K-Y, Li K, Liu B (2010) Cationic oligofluorene-substituted polyhedral oligomeric silsesquioxane as light-harvesting unimolecular nanoparticle for fluorescence amplification in cellular imaging. Adv Mater 22:643–646
Tang F, He F, Cheng H, Li L (2010) Self-assembly of conjugated polymer-Ag@SiO2 hybrid fluorescent nanoparticles for application to cellular imaging. Langmuir 26:11774–11778
Parthasarathy A, Ahn H-Y, Belfield KD, Schanze KS (2010) Two-photon excited fluorescence of a conjugated polyelectrolyte and its application in cell imaging. ACS Appl Mater Interfaces 2:2744–2748
Wu C, Bull B, Szymanski C, Christensen K, McNeill J (2008) Multicolor conjugated polymer dots for biological fluorescence imaging. ACS Nano 2:2415–2423
Wu C, Bull B, Christensen K, McNeill J (2009) Ratiometric single-nanoparticle oxygen sensors for biological imaging. Angew Chem Int Ed 48:2741–2745
Kim I-B, Shin H, Garcia AJ, Bunz UHF (2007) Use of a folate-PPE conjugate to image cancer cells in vitro. Bioconjugate Chem 18:815–820
Pu K-Y, Shi J, Cai L, Li K, Liu B (2011) Affibody-attached hyperbranched conjugated polyelectrolyte for targeted fluorescence imaging of her2-positive cancer cell. Biomacromolecules 12:2966–2974
Wu C, Schneider T, Zeigler M, Yu J, Schiro PG, Burnham DR, McNeill JD, Chiu DT (2010) Bioconjugation of ultrabright semiconducting polymer dots for specific cellular targeting. J Am Chem Soc 132:15410–15417
Ding D, Pu K-Y, Li K, Liu B (2011) Conjugated oligoelectrolyte-polyhedral oligomeric silsesquioxane loaded pH-responsive nanoparticles for targeted fluorescence imaging of cancer cell nucleus. Chem Commun 47:9837–9839
Li K, Pan J, Feng S–S, Wu AW, Pu K-Y, Liu Y, Liu B (2009) Generic strategy of preparing fluorescent conjugated-polymer-loaded poly(DL-lactide-co-Glycolide) nanoparticles for targeted cell imaging. Adv Funct Mater 19:3535–3542
Li K, Liu Y, Pu K-Y, Feng S–S, Zhan R, Liu B (2011) Polyhedral oligomeric silsesquioxanes-containing conjugated polymer loaded plga nanoparticles with trastuzumab (herceptin) functionalization for her2-positive cancer cell detection. Adv Funct Mater 21:287–294
Pu K-Y, Li K, Liu B (2010) Multicolor conjugate polyelectrolyte/peptide complexes as self-assembled nanoparticles for receptor-targeted cellular imaging. Chem Mater 22:6736–6741
Li K, Zhan R, Feng S–S, Liu B (2011) Conjugated polymer loaded nanospheres with surface functionalization for simultaneous discrimination of different live cancer cells under single wavelength excitation. Anal Chem 83:2125–2132
Zambianchi M, Maria FD, Cazzato A, Gigli G, Piacenza M, Sala FD, Barbarella G (2009) Microwave-assisted synthesis of thiophene fluorophores, labeling and multilabeling of monoclonal antibodies, and long lasting staining of fixed cells. J Am Chem Soc 131:10892–10900
Kandel PK, Fernando LP, Ackroyd PC, Christensen KA (2011) Incorporating functionalized polyethylene glycol lipids into reprecipitated conjugated polymer nanoparticles for bioconjugation and targeted labeling of cells. Nanoscale 3:1037–1045
Wu C, Jin Y, Schneider T, Burnham DR, Smith PB, Chiu DT (2010) Ultrabright and bioorthogonal labeling of cellular targets using semiconducting polymer dots and click chemistry. Angew Chem Int Ed 49:9436–9440
Lee K, Lee J, Jeong EJ, Kronk A, Elenitoba-Johnson KSJ, Lim MS, Kim J (2012) Conjugated polyelectrolyte-antibody hybrid materials for highly fluorescent live cell-imaging. Adv Mater 24:2479–2484
Bjork P, Nilsson KPR, Lenner L, Kagedal B, Persson B, Inganas O, Jonasson J (2007) Conjugated polythiophene probes target lysosome-related acidic vacuoles in cultured primary cells. Mol Cell Probes 21:329–337
Wang B, Zhu C, Liu L, Lv F, Yang Q, Wang S (2013) Synthesis of a new conjugated polymer for cell membrane imaging by using an intracellular targeting strategy. Polym Chem. doi: 10.1039/C3PY00097D
Liu L, Duan X, Liu H, Wang S, Li Y (2008) Microorganism-based assemblies of luminescent conjugated polyelectrolytes. Chem Commun 45:5999–6001
Giorgetti E, Giusti A, Arias E, Moggio I, Ledezma A, Romero J, Saba M, Quochi F, Marceddu M, Gocalinska A, Mura A, Bongiovanni G (2009) In situ production of polymer-capped silver nanoparticles for optical biosensing. Macromol Symp 283–284:167–173
Feng X, Yang G, Liu L, Lv F, Yang Q, Wang S, Zhu D (2012) A convenient preparation of multi-spectral microparticles by bacteria-mediated assemblies of conjugated polymer nanoparticles for cell imaging and barcoding. Adv Mater 24:637–641
Klingstedt T, Nilsson KPR (2012) Luminescent conjugated poly- and oligo-thiophenes: optical ligands for spectral assignment of a plethora of protein aggregates. Biochem Soc Trans 40:704–710
Wu C, Hansen SJ, Hou Q, Yu J, Zeigler M, Jin Y, Burnham DR, McNeill JD, Olson JM, Chiu DT (2011) Design of highly emissive polymer dot bioconjugates for in vivo tumor targeting. Angew Chem Int Ed 50:3430–3434
Ding D, Li K, Zhu Z, Pu K-Y, Hu Y, Jiang X, Liu B (2011) Conjugated polyelectrolyte-cisplatin complex nanoparticles for simultaneous in vivo imaging and drug tracking. Nanoscale 3:1997–2002
Li K, Ding D, Huo D, Pu K-Y, Thao NNP, Hu Y, Li Z, Liu B (2012) Conjugated polymer based nanoparticles as dual-modal probes for targeted in vivo fluorescence and magnetic resonance imaging. Adv Funct Mater 22:3107–3115
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2013 The Author(s)
About this chapter
Cite this chapter
Wang, S., Lv, F. (2013). Fluorescence Imaging Applications of Functionalized Conjugated Polyelectrolytes. In: Functionalized Conjugated Polyelectrolytes. SpringerBriefs in Molecular Science. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-40540-2_3
Download citation
DOI: https://doi.org/10.1007/978-3-642-40540-2_3
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-40539-6
Online ISBN: 978-3-642-40540-2
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)