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Photophysics of Ionic Biochromophores pp 141–153Cite as

UV–Visible Absorption Spectroscopy of Protein Ions

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Part of the book series: Physical Chemistry in Action ((PCIA))

Abstract

Optical spectroscopy has contributed enormously to our knowledge of the structure and dynamics of atoms and molecules and is now emerging as a cornerstone of the gas-phase methods available for investigating biomolecular ions. This chapter focuses on the UV and visible spectroscopy of peptide and protein ions stored in ion traps. First, we discuss experimental set-ups, de-excitation mechanisms following photo-excitation in electronically excited states and principles of action spectroscopy. Then, we report action spectra for different classes of gas-phase peptides and proteins. The optical activity of proteins in the near UV is directly related to the electronic structure and optical absorption of aromatic amino acids (Trp, Phe and Tyr). Some proteins also show absorption in the visible range due to the presence of a prosthetic group. Influence of protein charge state, formation of radical aromatic amino acids and solvation on proteins’ visible and UV spectra is discussed.

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Authors and Affiliations

  1. Institut Lumière Matière, UMR5306 Université Lyon 1-CNRS Université de Lyon, 69622, Villeurbanne Cedex, France

    Rodolphe Antoine & Philippe Dugourd

Authors
  1. Rodolphe Antoine
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  2. Philippe Dugourd
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Correspondence to Rodolphe Antoine .

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Editors and Affiliations

  1. Department of Physics and Astronomy, Aarhus University Faculty of Science, Aarhus, Denmark

    Steen Brøndsted Nielsen

  2. Department of Physics and Astronomy, Aarhus University Faculty of Science, Aarhus C, Denmark

    Jean Ann Wyer

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Antoine, R., Dugourd, P. (2013). UV–Visible Absorption Spectroscopy of Protein Ions. In: Brøndsted Nielsen, S., Wyer, J. (eds) Photophysics of Ionic Biochromophores. Physical Chemistry in Action. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-40190-9_8

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