Skip to main content

Platelet-Rich Plasma and Tissue Engineering

  • Chapter
  • First Online:
Platelet-Rich Plasma

Abstract

The focus in this chapter is an evaluation, based in data from the literature, of the use of Platelet-Rich Plasma (PRP) in cell culture for treatment of bone and cartilage defects. For the preparation of this chapter the data bank from PubMed, developed by the National Center for Biotechnology Information, U.S. National Library of Medicine was used as a tool. Key words used were: platelet-rich plasma, mesenchymal stem cells, biomaterials, tissue engineering; cartilage repair; bone healing. The role of PRP on tissue regeneration, cell proliferation and mesenchymal stem-cells is emphasized. In vitro and in vivo studies of PRP in bone and cartilage regeneration are described and referenced.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Agacayak S, Gulsun B, Ucan MC, Karaoz E, Nergiz Y (2012) Effects of mesenchymal stem cells in critical size bone defect. Eur Rev Med Pharmacol Sci 16(5):679–686

    Google Scholar 

  • Arpornmaeklong P, Kochel M, Depprich R, Kubler NR, Wurzler KK (2004) Influence of platelet-rich plasma (PRP) on osteogenic differentiation of rat bone marrow stromal cells. An in vitro study. Int J Oral Maxillofac Surg 33(1):60–70

    Article  Google Scholar 

  • Bi L, Cheng W, Fan H et al (2010) Reconstruction of goat tibial defects using an injectable tricalcium phosphate/chitosan in combination with autologous platelet-rich plasma. Biomaterials 31(12):3201–3211

    Article  Google Scholar 

  • Chen JC, Ko CL, Shih CJ, Tien YC, Chen WC (2012) Calcium phosphate bone cement with 10 wt% platelet-rich plasma in vitro and in vivo. J Dent 40(2):114–122

    Article  Google Scholar 

  • Chiu RC (2003) Bone marrow stem cells as a source for cell therapy. Heart Fail Rev 8:247–251

    Article  Google Scholar 

  • Cole BJ, Seroyer ST, Filardo G et al (2010) Platelet-rich plasma: where are we now and where are we going? Sports Health Multi Approach 2(3):203–210

    Article  Google Scholar 

  • Dolder JVD, Mooren R, Vloon APG et al (2006) Platelet-rich plasma: quantification of growth factor levels and the effect on growth and differentiation of rat bone marrow cells. Tissue Eng 12(11):3067–3073

    Article  Google Scholar 

  • Drengk A, Zapf A, Stürmer EK et al (2009) Influence of platelet-rich plasma on chondrogenic differentiation and proliferation of chondrocytes and mesenchymal stem cells. Cells Tissues Organs 189(5):317–326

    Article  Google Scholar 

  • Elbackly RM, Zaky SH, Muraglia A, Tonachini L, Brun F, Canciani B, Chiapale D, Santolini F, Cancedda R, Mastrogiacomo M (2012) A platelet rich plasma (prp) based membrane as a periosteal substitute with enhanced osteogenic and angiogenic properties: a new concept for bone repair. Tissue Eng Part A 19(1–2):152–165

    Google Scholar 

  • Erdo FC, Buhrle J, Blunk M (2003) Host-dependent tumorigenesis of embryonic stem cell transplantation in experimental stroke. J Cereb Blood Flow Metab 23(7):780–785

    Google Scholar 

  • Finkemeier CG (2002) Bone-grafiting and bone-graft substitutes. J Bone Joint Surg Am 84(3):454–464

    Google Scholar 

  • Hosgör F, Yılmaz N, Senyurt O, Gümüsova S, Cam B, Ceylan G, Yardımcı C, Pınarlı FA (2012) Effect of osteoblast cell culture on the bone implant contact. Acta Odontol Scand 71(3–4):626–631

    Google Scholar 

  • Huang Q, Wang Y, Wu T et al (2008) Preliminary separation of the growth factors in platelet-rich plasma: effects on the proliferation of human marrow-derived mesenchymal stem cells. Chin Med J 122(1):83–87

    Google Scholar 

  • Hunziker EB (2002) Articular cartilage repair: basic science and clinical progress. A review of the current status and prospects. Osteoarthr Cartil 10(6):432–463

    Google Scholar 

  • Hutmacher DW (2000) Scaffolds in tissue engineering bone and cartilage. Biomaterials 21(24):2529–2543

    Article  Google Scholar 

  • Kasten P, Beverungen M, Lorenz H, Wieland J, Fehr M, Geiger F (2012) Comparison of platelet-rich plasma and VEGF-transfected mesenchymal stem cells on vascularization and bone formation in a critical-size bone defect. Cells Tissues Organs 196(6):523–533

    Article  Google Scholar 

  • Kasten P, Vogel J, Beyen I et al (2008) Effect of platelet-rich plasma on the in vitro proliferation and osteogenic differentiation of human mesenchymal stem cells on distinct calcium phosphate scaffolds: the specific surface area makes a difference. J Biomater Appl 21(4):386–389

    Google Scholar 

  • Lei H, Xiao R, Tang XJ, Gui L (2009) Evaluation of the efficacy of platelet-rich plasma in delivering BMSCs into 3D porous scaffolds. J Biomed Mater Res B Appl Biomater 91(2):679–691

    Article  Google Scholar 

  • Lucarellia E, Beccheronia A, Donatib D et al (2003) Platelet-derived growth factors enhance proliferation of human stromal stem cells. Biomaterials 24(18):3095–3100

    Article  Google Scholar 

  • Marx RE (2004) Platelet-rich plasma: evidence to suport its use. J Oral Maxillofac Surg 62(4):489–496

    Article  Google Scholar 

  • Mooren RE, Dankers AC, Merkx MA et al (2010) The effect of platelet-rich plasma on early and late bone healing using a mixture of particulate autogenous cancellous bone and Bio-Oss: an experimental study in goats. Int J Oral Maxillofac Surg 39(4):371–378

    Article  Google Scholar 

  • Nikolidakis D, Dolder J, Wolke JG et al (2006) The effect of platelet-rich plasma on the bone healing around calcium phosphate-coated and non-coated oral implants in trabecular bone. Tissue Eng 12(9):2555–2563

    Article  Google Scholar 

  • Okuda K, Kawase T, Momose M et al (2003) Platelet rich plasma contains high levels of platelet derived growth factors and transforming growth factor beta and modulates the proliferation of periodontal related cells in vitro. J Periodontal 74(6):849–857

    Article  Google Scholar 

  • Qi YY, Chen X, Jiang YZ et al (2009) Local delivery of autologous platelet in collagen matrix simulated in situ articular cartilage repair. Cell Transplant 18(10):1161–1169

    Article  Google Scholar 

  • Rai B, Ho KH, Lei Y et al (2007a) Polycaprolactone-20% tricalcium phosphate scaffolds in combination with platelet-rich plasma for the treatment of critical-sized defects of the mandible: a pilot study. J Oral Maxillofac Surg 65(11):2195–2205

    Article  Google Scholar 

  • Rai B, Oest ME, Dupont KM et al (2007b) Combination of platelet-rich plasma with polycaprolactone-tricalcium phosphate scaffolds for segmental bone defect repair. J Biomed Mater Res A 81(4):888–899

    Article  Google Scholar 

  • Robey PG (2000) Stem cells near the century mark. J Clin Invest 105(11):1489–1491

    Article  Google Scholar 

  • Solchaga LA, Dennis JE, Goldberg VM et al (1999) Hyaluronic acid-based polymers as cell carriers for tissue-engineered repair of bone and cartilage. J Orthop Res 17:205–213

    Article  Google Scholar 

  • Tönzüm TF, Demiralp B (2003) Platelet-rich plasma: a promising innovation in dentistry. J Can Dent Asscosc 69(10):664a–664h

    Google Scholar 

  • Urgate DE, Alfonso Z, Zuk PA et al (2003a) Differential expression of stem cells mobilization-associated molecules on multilineage cells from adipose tissue and bone marrow. Immunol Lett 89(2–3):267–270

    Google Scholar 

  • Urgate DE, Morizono K, Elbarbary A et al (2003b) Comparison of multi-lineage cells from human adipose tissue and bone marrow. Cells Tissues Organs 174(3):101–109

    Google Scholar 

  • Wei Wu, Chen Fulin, Liu Yanpu et al (2007) Autologous injectable tissue-engineered cartilage by using platelet-rich plasma: experimental study in a rabbit model. J Oral Maxillofac Surg 65:1951–1957

    Article  Google Scholar 

  • Wiltfang J, Kloss FR, Kessler P et al (2004) Effects of platelet-rich plasma no bone healing in combination with autogenous bone and bone substitutes in critical-size defects: an animal experiments. Clin Oral Implant Res 15(2):187–193

    Article  Google Scholar 

  • Xie X, Wang Y, Zhao C, Guo S, Liu S, Jia W, Tuan RS, Zhang C (2012) Comparative evaluation of MSCs from bone marrow and adipose tissue seeded in PRP-derived scaffold for cartilage regeneration. Biomaterials 33(29):7008–7018

    Article  Google Scholar 

  • Yang PJ, Temenoff JS (2009) Engineering orthopedic tissue interfaces. Tissue Eng Part B 15(2):127–141

    Article  Google Scholar 

  • Zhang S, Mao T, Chen F (2011a) Influence of platelet-rich plasma on ectopic bone formation of bone marrow stromal cells in porous coral. Int J Oral Maxillofac Sur 40(9):961–965. doi:10.1016/j.ijom.2011.02.037

    Article  Google Scholar 

  • Zhang YD, Wang G, Sun Y et al (2011b) Combination of platelet-rich plasma with degradable bioactive borate glass for segmental bone defect repair. Acta Orthop Belg 77(1):110–115

    MathSciNet  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to William D. Belangero .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Rodrigues, A.A. et al. (2014). Platelet-Rich Plasma and Tissue Engineering. In: Lana, J., Andrade Santana, M., Dias Belangero, W., Malheiros Luzo, A. (eds) Platelet-Rich Plasma. Lecture Notes in Bioengineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-40117-6_5

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-40117-6_5

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-40116-9

  • Online ISBN: 978-3-642-40117-6

  • eBook Packages: EngineeringEngineering (R0)

Publish with us

Policies and ethics