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Genomics and Spectroscopy Provide Novel Insights into the Mechanisms of Litter Decomposition and Nitrogen Assimilation by Ectomycorrhizal Fungi

  • Anders Tunlid
  • Francois Rineau
  • Mark Smits
  • Firoz Shah
  • Cesar Nicolas
  • Tomas Johansson
  • Per Persson
  • Francis Martin
Chapter
Part of the Soil Biology book series (SOILBIOL, volume 36)

Abstract

The majority of nitrogen in forest soils is found in organic form, primarily as proteins. This nitrogen is mobilized and becomes available to trees as a result of the depolymerizing activities of symbiotic ectomycorrhizal fungi. To capture the nitrogen, the fungi must at least partly disrupt the recalcitrant organic matter–protein complexes within which the nitrogen is embedded. In this review, we will describe how a combination of spectroscopic methods and transcriptome analyses has provided novel insights into the mechanisms by which the ectomycorrhizal fungus Paxillus involutus decomposes organic matter when acquiring nitrogen from plant litter. The observed chemical changes were consistent with a hydroxyl-radical attack, involving Fenton chemistry similar to that of saprophytic brown-rot fungi. Unlike the saprophytic fungi, P. involutus did not show any expression of genes encoding extracellular enzymes needed to metabolize the released carbon. We suggest that these activities have been lost in ectomycorrhizal fungi as an adaptation to symbiotic growth on host photosynthate. Indeed experiments have shown that the decomposition of plant litter and assimilation of nitrogen are triggered by the addition of glucose. In contrast, the addition of ammonium, the most abundant inorganic N form in forest soils, had relatively minor effects of the decomposition of litter material by P. involutus. The data suggest that the expression of the decomposition and nitrogen assimilation processes can be tightly regulated by the host carbon supply. Finally, the prospects of using novel spectroscopic methods and transcriptomic data to identify specific transcripts or chemical signatures that can be used as biomarkers for probing the activity of mycorrhizal fungi in the field are discussed.

Keywords

Soil Organic Matter Arbuscular Mycorrhizal Mycorrhizal Fungus Litter Decomposition Litter Material 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The work has been supported by grants from the Swedish Research Council (VR), the strategic research program Biodiversity and Ecosystem Services in a Changing Climate (BECC) and the Danish Agency for Science and Technology, and the Research Foundation—Flanders (FWO). The genome and transcriptome sequencing of Paxillus involutus was funded by grants from the U.S. Department of Energy.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Anders Tunlid
    • 1
  • Francois Rineau
    • 2
  • Mark Smits
    • 2
  • Firoz Shah
    • 1
  • Cesar Nicolas
    • 1
  • Tomas Johansson
    • 1
  • Per Persson
    • 1
    • 3
  • Francis Martin
    • 4
  1. 1.Department of Biology, Microbial Ecology GroupLund UniversityLundSweden
  2. 2.Centre for Environmental SciencesHasselt UniversityDiepenbeekBelgium
  3. 3.Centre for Environmental and Climate Research (CEC)Lund UniversityLundSweden
  4. 4.INRA-Nancy, Lab of Excellence ARBREUMR INRA-University of Lorraine ‘Interactions Arbres/Micro-organismes’ChampenouxFrance

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