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Methods in Fungal Genetics

  • Kevin McCluskey
  • Aric Wiest
Chapter
Part of the Soil Biology book series (SOILBIOL, volume 36)

Abstract

The myriad uses of filamentous and yeastlike fungi have spawned industry and investigation touching on every aspect of human endeavor. Much like the domestication of livestock and crop plants, humans have domesticated and improved fungi for specific and general use. In the modern biotechnological era, these improvements include strain characterization and standardization as well as strain improvement through genetic and molecular methods. Underpinning this improvement are biological materials whose shared use comprises a molecular toolkit for manipulation of filamentous fungi. These include various dominant and recessive selectable markers, gene targeting systems, protein expression and tagging vectors, and the mutated or engineered strains designed for these vectors. Some of these are based on features of the fungal lifestyle, while others were imported by analogy from bacterial, plant, or animal systems. Together they comprise a flexible and powerful molecular genetic framework for the development of highly productive strains and culture systems as fungi are used as foundations of the bio-economy of the twenty-first century.

Keywords

Filamentous Fungus Selectable Marker Aspergillus Nidulans Strain Improvement Mitotic Recombination 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Fungal Genetics Stock CenterUniversity of MissouriKansas CityUSA

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