Estimating Viral Haplotypes in a Population Using k-mer Counting

  • Raunaq Malhotra
  • Shruthi Prabhakara
  • Mary Poss
  • Raj Acharya
Conference paper
Part of the Lecture Notes in Computer Science book series (LNCS, volume 7986)

Abstract

Viral haplotype estimation in a population is an important problem in virology. Viruses undergo a high number of mutations and recombinations during replication for their survival in host cells and exist as a population of closely related genetic variants. Due to this, estimating the number of haplotypes and their relative frequencies in the population becomes a challenging task. The usage of a sequenced reference genome has its limitations due to the high mutational rates in viruses. We propose a method for estimating viral haplotypes based only on the counts of k-mers present in the viral population without using the reference genome. We compute k-mer pairs that are related to each other by one mutation, and compute a minimal set of viral haplotypes that explain the whole population based on these k-mer pairs. We compare our method to the software ShoRAH (which uses a reference genome) on simulated dataset and obtained comparable results, even without using a reference genome.

Keywords

viral haplotype estimation structural variants detection k-mer counting variant detection greedy generating set algorithm 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Raunaq Malhotra
    • 1
  • Shruthi Prabhakara
    • 1
  • Mary Poss
    • 2
  • Raj Acharya
    • 1
  1. 1.Department of Computer Science and EngineeringPennsylvania State UniversityUniversity ParkUSA
  2. 2.Department of Biology, Center for Infectious Disease DynamicsPennsylvania State UniversityUniversity ParkUSA

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