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Nano-encapsulation of Oligonucleotides for Therapeutic Use

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Nucleic Acid Nanotechnology

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 29))

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Abstract

Oligonucleotides got more and more into focus for therapeutic purposes. Administration of such molecules is a challenge, as surviving the bloodstream passage and passing the barrier cell membrane are almost insuperable tasks. Although successful clinical studies have been conducted with naked oligonucleotides, such as antisense agents or siRNA, poor cellular uptake and low cellular persistence reveal the need for adequate carriers.

Delivery of the undamaged oligonucleotide to its site of action has been explored with manifold systems. However, these systems all have one aim: protection of the cargo during the bloodstream passage, facilitation of cellular uptake, and, finally, payload release into the cytosol. Size plays also an important role for the physiological pathway, as particles, if their size is suboptimal, may either clog blood vessels, be removed by the reticuloendothelial system, or undergo rapid renal clearance.

Therefore, research in this field takes advantage of natural nucleic acid encapsulation systems (viruses) or aims at mimicking virus-like features with nonviral carriers. This review focuses on the principles of oligonucleotide encapsulation or packaging with different classes of carrier molecules towards therapeutic use.

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Authors and Affiliations

  1. Pharmaceutical Biotechnology, Ludwig Maximilians University Munich, Munich, Germany

    Eveline Edith Salcher & Ernst Wagner

  2. Center for System-Based Drug Research, Ludwig Maximilians University Munich, Munich, Germany

    Eveline Edith Salcher & Ernst Wagner

  3. Center for Nanoscience (CeNS), Ludwig Maximilians University Munich, Munich, Germany

    Eveline Edith Salcher & Ernst Wagner

  4. Nanosystems Initiative Munich (NIM), Munich, Germany

    Eveline Edith Salcher & Ernst Wagner

Authors
  1. Eveline Edith Salcher
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  2. Ernst Wagner
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Correspondence to Eveline Edith Salcher .

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Editors and Affiliations

  1. Dept. of Molecular Biology, University of Aarhus, Center for DNA Nanotechnology, Interdisciplinary Nanoscience Center, Aarhus C, Denmark

    Jørgen Kjems

  2. Center for DNA Nanotechnology, Interdisciplinary Nanoscience Center, Aarhus University, Aarhus C, Denmark

    Elena Ferapontova

  3. Center for DNA Nanotechnology, Interdisciplinary Nanoscience Center and Department of Chemistry, Aarhus University, Aarhus C, Denmark

    Kurt V. Gothelf

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Salcher, E.E., Wagner, E. (2014). Nano-encapsulation of Oligonucleotides for Therapeutic Use. In: Kjems, J., Ferapontova, E., Gothelf, K. (eds) Nucleic Acid Nanotechnology. Nucleic Acids and Molecular Biology, vol 29. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-38815-6_9

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