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Micro-Segmented Flow pp 203–230Cite as

Characterization of Combinatorial Effects of Toxic Substances by Cell Cultivation in Micro Segmented Flow

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Part of the book series: Biological and Medical Physics, Biomedical Engineering ((BIOMEDICAL))

Abstract

This chapter reviews the application of micro segmented flow for the screening of toxic effects on bacteria, eukaryotic microorganisms, human cells and multicellular systems. Besides, the determination of complete dose/response functions of toxic substances with a minimum of cells and chemicals, it is reviewed how two- and multi-dimensional concentration spaces can be screened in order to evaluate combinatorial effects of chemicals on cells. The challenge for the development of new and miniaturized methods is derived from the increase of the number of different used substances in technique, agriculture and medicine, from the increasing release of new substances and nanomaterials into our environment and from the improvement of the insight of toxicity of natural substances and the interferences between different substances resulting in toxic effects on different organisms, cells and tissues. The application of two-dimensional toxicological screenings on selected examples of effector combinations is described. Examples for the detection of an independent, an additive and a synergistic interference between two substances are given. In addition, it is shown that the screening for toxicological effects in complete two-dimensional concentration spaces allows the detection of complex response behaviour—for example, the formation of tolerances and stimulation peaks—which thereby can be characterized. The characterization of interference of toxic organic substances with silver nanoparticles is reported as an example for the potential of micro segmented-flow technique for evaluating the toxicological impact of new materials. Finally, it is demonstrated that the technique can be applied for different organisms like simple bacteria, single cell alga such as Chlorella vulgaris and multicellular systems up to the development of complete organisms beginning from eggs.

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Acknowledgments

This research was financially supported by the German Federal Environmental Foundation (DBU) and by the BMBF (OPTIMI project KFZ-16SV3701 and the BactoCat project KFZ-031A161A).

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Authors and Affiliations

  1. Ilmenau University of Technology, Institute of Chemistry and Biotechnology, PF 10 05 65, 98684 , Ilmenau, Germany

    J. Cao, D. Kürsten, A. Funfak, S. Schneider & J. M. Köhler

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  1. J. Cao
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  2. D. Kürsten
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  3. A. Funfak
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  4. S. Schneider
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  5. J. M. Köhler
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Correspondence to J. Cao .

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Editors and Affiliations

  1. Technical University Ilmenau Institute of Chemistry and Biotechnology, Ilmenau, Germany

    J. Michael Köhler

  2. Institute for Bioprocessing and Analytical Measurement Techniques, Heilbad Heiligenstadt, Germany

    Brian P. Cahill

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Cao, J., Kürsten, D., Funfak, A., Schneider, S., Köhler, J.M. (2014). Characterization of Combinatorial Effects of Toxic Substances by Cell Cultivation in Micro Segmented Flow. In: Köhler, J., Cahill, B. (eds) Micro-Segmented Flow. Biological and Medical Physics, Biomedical Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-38780-7_8

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  • DOI: https://doi.org/10.1007/978-3-642-38780-7_8

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