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Heterologous Protein Expression and Secretion in Yarrowia lipolytica

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Yarrowia lipolytica

Part of the book series: Microbiology Monographs ((MICROMONO,volume 25))

Abstract

Heterologous protein production constitutes an important research field, having both academic and commercial applications. The use of yeasts as host systems took advantage of the combination of their easy manipulation and high growth capacity, with their eukaryotic subcellular organisation allowing post-translational processing. Among available yeast host systems, Yarrowia lipolytica (Yarrowia) appears as one of the most attractive. This non-conventional dimorphic yeast has been distinguished due to its remarkable regularity of performance in the efficient secretion of various heterologous proteins. We will present in this chapter Yarrowia’s characteristics regarding heterologous protein production and describe the genetic and molecular tools available. Recent developments of the Yarrowia expression system, such as surface display vectors, engineered strains and high-throughput screening processes, will be reviewed. A comprehensive survey of the literature allowed us to list more than 100 heterologous proteins, from more than 60 species, successfully produced until now in this yeast. This amount of data sometimes offers a comparison with other yeast host systems and globally demonstrates the reliability and versatility of Yarrowia as host for heterologous production.

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Acknowledgements

We wish to thank all colleagues and collaborators who accepted to communicate unpublished data: Dr. Martine Chartier (Laboratoire d’Ingénierie des Systèmes Macromoléculaires, CNRS, Marseille, France), Prof. Zhen-Ming Chi (Unesco Chinese Center of Marine Biotechnology and Institute of Marine Biodiversity and Evolution, Ocean University of China, Qingdao, China), Dr. Ricardo Cordero (former PhD student from our laboratory), Dr. Art Franke (Pfizer, USA), Dr. Christof Gysler (Nestec, Switzerland), Dr. Nicole Houba-Hérin (Laboratoire de Biologie Cellulaire, INRA, Versailles, France), Dr. Claude Jolivalt (ENSCP Chimie ParisTech, Laboratoire Charles Friedel, Paris, France), Dr. Gotthard Kunze (Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany), Dr. Nathalie Libessart (Roquette Frères, France), Dr. Bi-Ching Sang (Pharmingen, USA), Dr. Dominique Swennen (our laboratory), Dr. Kohji Uchida (Oriental Yeast, Japan) and Dr. Petrus J. van Zyl (Bioprocess Development, CSIR Biosciences, Modderfontein, South Africa).

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  1. INRA, UMR1319 Micalis, Domaine de Vilvert, 78352, Jouy-en-Josas, France

    Catherine Madzak & Jean-Marie Beckerich

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  1. TU Dresden Inst. Mikrobiologie, Dresden, Sachsen, Germany

    Gerold Barth

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Madzak, C., Beckerich, JM. (2013). Heterologous Protein Expression and Secretion in Yarrowia lipolytica . In: Barth, G. (eds) Yarrowia lipolytica. Microbiology Monographs, vol 25. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-38583-4_1

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