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Study on Antibacterial Activity of Radix isatidis Extracts and Preliminary Investigation of Their Antibacterial Mechanism

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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 251))

Abstract

The antibacterial mechanism of Radix isatidis extracts against Escherichia coli and Bacillus subtilis was investigated by determining the changes in electrical conductivity of bacterial suspension treated by Radix isatidis extracts, as well as the changes of phosphorus metabolism and protein expression. The results showed that the electrical conductivity of treated bacterial suspension was significantly increased, indicating that Radix isatidis extracts could damage the integrity of cell membrane. In addition, the consumption of phosphorus was decreased in the Radix isatidis-treated bacteria, which influenced the synthesis of important cellular components, such as phospholipid, energy metabolism. Coomassie brilliant blue G-250 colorimetric assay demonstrated that Radix isatidis extracts could block the expression of some proteins in bacterial cells.

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Acknowledgments

The research was supported by Tianjin Agricultural Sciences Research Development Fund evolution, the item No. is 2011N08.

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Authors and Affiliations

  1. Department of Basic Science, Tianjin Agricultural University, Tianjin, 300384, People’s Republic of China

    Lin Tian & Zhan Wang

Authors
  1. Lin Tian
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  2. Zhan Wang
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Corresponding author

Correspondence to Lin Tian .

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Editors and Affiliations

  1. College of Bioengineering, Tianjin University of Science and Technology, Tianjin, People's Republic of China

    Tong-Cun Zhang

  2. Nanjing University of Technology, Nanjing, People's Republic of China

    Pingkai Ouyang

  3. Department of Microbiology & Molecular Genetics, University of Texas- Houston Medical School, Texas, Texas, USA

    Samuel Kaplan

  4. Wellcome Trust Sanger Institute, Cambridge, United Kingdom

    Bill Skarnes

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Tian, L., Wang, Z. (2014). Study on Antibacterial Activity of Radix isatidis Extracts and Preliminary Investigation of Their Antibacterial Mechanism. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 251. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37925-3_180

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  • DOI: https://doi.org/10.1007/978-3-642-37925-3_180

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-37924-6

  • Online ISBN: 978-3-642-37925-3

  • eBook Packages: EngineeringEngineering (R0)

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