Abstract
Polyprenols are new effective lipids separated from Ginkgo biloba L. leaves. In this paper, the light and heavy distillates were prepared from ginkgo lipids by extraction with petroleum ether, saponification, and molecular distillation. And then the seven known compounds: β-sitosterol acetate (1), palmitamide (2), glyceryl tripalmitate (3), β-sitosterol-3-O-β-d-glucopyranoside (4), β-sitosterol (5), stigmasterol (6), ergosterol (7), and polyprenols were isolated by chromatograph from lipids of Ginkgo biloba L. leaves. In the meantime, the antibacterial activity of light and heavy distillates, seven compounds and polyprenols was assessed against three pathogenic strains (Staphylococcus aureus, Escherichia coli, and Bacillus subtilis) employing disc-diffusion and broth-dilution assays. The heavy distillates showed the highest activity (zone of inhibition of 14–17 mm) followed by the light distillates (13–15 mm), polyprenols (12–14 mm), β-sitosterol (5) (10–14 mm), palmitamide (2) (11–13 mm), β-sitosterol-3-O-β-d-glucopyranoside (4) (10–13 mm), β-sitosterol acetate (1) (10–12 mm), stigmasterol (6), and ergosterol (7) (9–10 mm), glyceryl tripalmitate (3) (10 mm) at 500 μg/mL. MIC and MBC values for the bacteria sensitive to polyprenols were in the range of 31.3–62.5 μg/mL and 125 μg/mL. It can be inferred polyprenols which have synergistic inhibitory effect with other lipids.
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The authors gratefully acknowledge the financial support by the National “Twelfth Five-Year” Plan for Science & Technology Support in China (No.2011BADB1B03).
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Tao, R., Wang, C., Kong, Z. (2014). Antibacterial Activity of Polyprenols and Other Lipids from Ginkgo biloba L. Leaves. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 251. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37925-3_169
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DOI: https://doi.org/10.1007/978-3-642-37925-3_169
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