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Expression of Recombinant Human Bone Morphogenetic Protein 2 in Insect Cells, Purification and Activity Analysis

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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 251))

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Abstract

The aim was to produce recombinant human bone morphogenetic protein 2 (BMP2) using baculovirus-insect cell protein expression system. The recombinant protein was purified by high affinity Ni-charged resin, then the bioactivity of recombinant protein was detected by alkaline phosphatase assay, and the result showed that the recombinant BMP2 stimulated alkaline phosphatase activity in MC3T3-E1 cells, which lay the foundation for further study and application of recombinant BMP2.

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Authors and Affiliations

  1. College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Wen Chu, Shenheng Luo, Lihong Xu, Minhui Long & Aipo Diao

Authors
  1. Wen Chu
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  2. Shenheng Luo
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  3. Lihong Xu
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  4. Minhui Long
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  5. Aipo Diao
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Corresponding author

Correspondence to Aipo Diao .

Editor information

Editors and Affiliations

  1. College of Bioengineering, Tianjin University of Science and Technology, Tianjin, People's Republic of China

    Tong-Cun Zhang

  2. Nanjing University of Technology, Nanjing, People's Republic of China

    Pingkai Ouyang

  3. Department of Microbiology & Molecular Genetics, University of Texas- Houston Medical School, Texas, Texas, USA

    Samuel Kaplan

  4. Wellcome Trust Sanger Institute, Cambridge, United Kingdom

    Bill Skarnes

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© 2014 Springer-Verlag Berlin Heidelberg

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Chu, W., Luo, S., Xu, L., Long, M., Diao, A. (2014). Expression of Recombinant Human Bone Morphogenetic Protein 2 in Insect Cells, Purification and Activity Analysis. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 251. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37925-3_140

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  • DOI: https://doi.org/10.1007/978-3-642-37925-3_140

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-37924-6

  • Online ISBN: 978-3-642-37925-3

  • eBook Packages: EngineeringEngineering (R0)

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