Abstract
The aim of this paper is to research the protective effect of Astragalus against intermittent hypoxia-induced hippocampal neurons impairment in rat and lay the theoretical foundation for sleep apnea improvement in cognitive function by Astragalus. Male Wistar rats were divided into four groups: (1) blank control group; (2) normoxia group; (3) intermittent hypoxia group; (4) Astragalus treated intermittent hypoxia group. After 6-week treatment, the expression of iNOS was detected by real-time reverse transcription polymerase chain reaction (RT-PCR) at mRNA level as well as by immunohistochemistry (IHC) and western blot at protein level. As a result, Astragalus reduced the expression of iNOS at mRNA and protein levels in hippocampus compared with non-treated groups (P < 0.05). In conclusion, Astragalus could protect intermittent hypoxia-induced hippocampal neurons impairment in rat.
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Acknowledgments
This work was supported by a grant 10JCYBJC25800 from Natural Science Foundation of Tianjin, a grant 20100132 from Tianjin higher school science and technology development fund project and a grant 2009KY17 from Tianjin Medical University Fund.
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Zhang, Q., Gao, W., Man, S., Zhang, Y., Chen, B. (2014). Inhibition of iNOS to Protect Intermittent Hypoxia-Induced Hippocampal Neurons Impairment by Astragalus Extract in Rat. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 250. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37922-2_96
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DOI: https://doi.org/10.1007/978-3-642-37922-2_96
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