Abstract
Cancer is one of the major causes of death worldwide. Many clinical evidences showed that the incidence of colon cancer increased after the cholecystectomy, suggesting that bile acid and its derivatives might play roles in the process of tumorigenesis and its prevention. Bile acid derivative is a kind of good candidates for prodrug design, but there are still few reports of its development for anticancer drugs either domestically or internationally. In the present study, IC50 of 9 bile acid derivatives against MCF-7 human breast cancer cells was detected by MTT assay, and the safety of several bile acid derivatives with high anticancer effect were further analyzed in human umbilical vein endothelial cells. The results showed that among 9 bile acid derivatives, cholic acid n-buthyl ester had lower IC50 against cancer cells, but it did not have cytotoxicity to normal cell at low concentration, indicating that they would be a good candidate for the development of novel anticancer agents.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kanavos P (2006) The rising burden of cancer in the developing world. Ann Oncol 6(17):15–23
Shibuya K, Mathers CD, Pinto BC (2002) Global and regional estimates of cancer mortality and incidence by site: II. results for the global burden of disease 2000. BMC Cancer 2:37
Salminen E, Izewska J, Andreo P (2005) IAEA’s Role in the global management of cancer-focus on upgrading radiotherapy services. Acta Oncol 44:816–824
Sener SF, Grey N (2005) The global burden of cancer. Surg Oncol 92:1–3
Sudhakar C, Sabitha P, Shashi KR (2007) Betulinic acid inhibits prostate cancer growth through inhibition of specificity protein transcription factors. Cancer Res 67:2816–2823
García SR, Ng C, Anderson H et al (2011) Synergistic drug combinations for tuberculosis therapy identified by a novel high-throughput screen. Antimicrob Agents Ch 55(8):3861–3869
Aydee CE, Syrovets T, Pitterle K et al (2010) Tirucallic acids are novel pleckstrin homology domain-dependent Akt inhibitors inducing apoptosis in prostate cancer cells. Mol Pharmacol 3(77):378–387
Gaube F, Wolfl S, Pusch L et al (2007) Gene expression profiling reveals effects of cimicifuga racemosa (L.) NUTT. (black cohosh) on the estrogen receptor positive human breast cancer cell line MCF-7. BMC Pharmacol 7:11
Pai R, Tarnawski AS, Tran T (2004) Deoxycholic acid activates β-catenin signaling pathway and increases colon cell cancer growth and invasiveness. Mol Biol Cell 15:2156–2163
Lazaro JE, Gay F (1998) Plasmodium falciparum: in vitro cytotoxicity testing using MTT. J Biomol Screen 1(3):49–53
Hida T, Ueda R, Takahashi T et al (1989) Chemosensitivity and radiosensitivity of small cell lung cancer cell lines studied by a newly developed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) hybrid assay. Cancer Res 9(49):4785–4790
Noguchi K, Tani MI, Nakamura M (2005) Evaluation of chemosensitivity testing with highly purified tumor cells in 435 patients with gastric carcinoma using an MTT assay. Anticancer Res 25:931–938
Montoro E, Lemus D, Echemendia M et al (2005) Comparative evaluation of the nitrate reduction assay, the MTT test, and the resazurin microtitre assay for drug susceptibility testing of clinical isolates of mycobacterium tuberculosis. J Antimicrob Chemth 2(55):500–505
Akiri SR, Sareddy GR, Phanithi PB et al (2010) The antioxidant and antiproliferative activities of methanolic extracts from njavara rice bran. BMC Complem Altern M 10:4
Pieters R, Loonen AH, Huismans DR et al (1990) In vitro drug sensitivity of cells from children with leukemia using the MTT assay with improved culture conditions. Blood 11(76):2327–2336
Lu J, Tan M, Huang WC et al (2009) Mitotic deregulation by survivinin ErbB2-overexpressing breast cancer cells contributesto taxol resistance. Clin Cancer Res 15(4):1326–1334
Nakamura R, Saikawa Y, Kubota T et al (2006) Role of the MTT chemosensitivity test in the prognosis of gastric cancer patients after postoperative adjuvant chemotherapy. Anticancer Res 26:1433–1438
Mueller H, Kassack MU, Wiese M (2004) Comparison of the usefulness of the MTT, ATP, and calcein assays to predict the potency of cytotoxic agents in various human cancer cell lines. J Biomol Screen 9(6):506–515
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63
Mukherjee P, Anthony CF, Laura M et al (2008) Ganglioside GM3 suppresses the proangiogenic effects of vascular endothelial growth factor and ganglioside GD1a. J Lipid Res 2(49):929–938
Acknowledgments
This work was financially supported by the National Basic Research Program of China (973 Program) (NO. 2009CB825504), the National Natural Science Fundation of China (NO. 31000343, NO. 31071126), the Science & Technology Project of Tianjin (No. 10JCZDJC22500,10ZXCXSY10100), and the High School Science & Technology Development Fundation of Tianjin (NO.20090602).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Luo, X., Wang, J., Gu, X., Zhang, C., Hu, X., Zhang, TC. (2014). The Analysis of the Inhibition Effect of Cholic Acid Derivatives on the Proliferation of Breast Cancer Cells. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 250. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37922-2_87
Download citation
DOI: https://doi.org/10.1007/978-3-642-37922-2_87
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-37921-5
Online ISBN: 978-3-642-37922-2
eBook Packages: EngineeringEngineering (R0)