Abstract
Myocardin-related transcription factor A (MRTF-A) is a transcriptional coactivator of serum response factor (SRF). Recent studies have found that the MRTF–SRF signal pathway might play critical role in Rho-dependent cytoskeletal rearrangements and tumor metastasis. To further explore the detailed molecular mechanisms for MRTF-A-mediated cell migration, in this study, the effect of MRTF-A on the migration of mouse fibroblast NIH3T3 cells and MCF-7 human breast cancer cells was detected using a scratch wound model, and its transcriptional activity on the promoter of Cysteine-rich angiogenic inducer 61 (CYR61) was also analyzed by luciferase assay. The results showed that overexpression of MRTF-A could significantly promote the migration of NIH3T3 cells and drive the transcriptional activity of CYR61 promoter in African green monkey kidney COS-7 cells and strongly enhance upregulation of CYR61 gene. In contrast, RNA interference-mediated knockdown of MRTF-A reduced the migration ability of MCF-7 breast cancer cells. Taken together, our results suggested that MRTF-A is involved in tumor metastasis via transactivation of CYR61.
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Luo, X. et al. (2014). MRTF-A Promotes Migration of MCF-7 Breast Cancer Cells via Transactivation of CYR61. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 250. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37922-2_86
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DOI: https://doi.org/10.1007/978-3-642-37922-2_86
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