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Using Water Miscible Ionic Liquid to Improve the Efficiency of 15α-hydroxylation of 13-ethyl-gon-4-en-3,17-dione by Penicillium raistrickii

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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 250))

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Abstract

Improved efficiency of steroid microbial transformation by co-solvents is generally attributed to their positive effects on the solubility of substrate in aqueous media. Ionic liquids have been widely researched as possible ‘green’ replacements for organic solvents. In this study, the effect of water miscible ionic liquids on the 15α-hydroxylation of 13-ethyl-gon-4-en-3,17-dione by Penicillium raistrickii ATCC 10490 was investigated. The results indicated that the striking improvement of biotransformation of substrates up to 94.46 % were obtained after addition of [BMIM]BF4 under the optimized conditions. This also resulted in morphological changes of P. raistrickii mycelia in the fermentation broth with or without [BMIM]BF4. Based on the above observations, we therefore conclude that [BMIM]BF4-induced changes of mycelial morphology of P. raistrickii have positive effects on the production of the desired product, thus most likely contributing to the observed higher yield of steroid biotransformation in addition to [BMIM]BF4 known effects on substrate solubility.

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Acknowledgments

This work was financially supported by National High-tech R&D Program of China (863 Program) “Biotransformation Technology of Steroids” (No. 2011AA02A211), Applied Basic Research Programs of Science and Technology Commission Foundation of Tianjin (No.12JCQNJC06400), Science & Technology Development Project of Tianjin Higher Education (No. 20100601).

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Authors and Affiliations

  1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Shuhong Mao, Boyuan Hua & Fuping Lu

  2. National Engineering Laboratory for Industrial Enzymes, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Na Wang, Zhijiang Ge & Fuping Lu

  3. Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Yanqing Li

Authors
  1. Shuhong Mao
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  2. Na Wang
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  3. Zhijiang Ge
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  4. Boyuan Hua
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  5. Yanqing Li
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  6. Fuping Lu
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Corresponding author

Correspondence to Fuping Lu .

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Editors and Affiliations

  1. College of Bioengineering ,Tianjin University of Science and Technology, Tianjin, People's Republic of China

    Tong-Cun Zhang

  2. Nanjing University of Technology, Nanjing, People's Republic of China

    Pingkai Ouyang

  3. Department of Microbiology & Molecular Genetics, University of Texas- Houston Medical School, Texas, Texas, USA

    Samuel Kaplan

  4. Wellcome Trust Sanger Institute, Cambridge, United Kingdom

    Bill Skarnes

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Mao, S., Wang, N., Ge, Z., Hua, B., Li, Y., Lu, F. (2014). Using Water Miscible Ionic Liquid to Improve the Efficiency of 15α-hydroxylation of 13-ethyl-gon-4-en-3,17-dione by Penicillium raistrickii . In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 250. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37922-2_84

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  • DOI: https://doi.org/10.1007/978-3-642-37922-2_84

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-37921-5

  • Online ISBN: 978-3-642-37922-2

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