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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012) pp 1003–1009Cite as

Different Chemicals Stimulate Diapause Termination of Artemia Embryos

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Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 250))

Abstract

This paper studied the effect of the chemicals on the diapause termination of the Artemia cysts, including reactive oxygen species (ROS) producing reagent CaO2, reactive nitric species (RNS) producing reagent NO, and antioxidant reagents tea polyphenol (TP) and ascorbic acid (VC). The results showed that all the tested chemicals could significantly promote Artemia cysts development, and the optimum range for CaO2, TP, VC, and sodium nitroprusside (SNP, NO generators) was 38–94, 50–150, 625–2500, and 25–100 mg/L, respectively. The hatching percentage (H %) was raised from 20 % for the control group to 86 % for the CaO2 treated group, 86 % for the TP treated group, 85 % for the VC treated group, and 67 % for the SNP treated group, respectively. An analysis of trehalose consumption and glycerol accumulation of the Artemia cysts was also performed during incubation.

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Acknowledgments

This study was supported by the International Cooperation Research Program of the Ministry of Science & Technology of China (grant number CK08-03 and 2010DFA32300).

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Authors and Affiliations

  1. College of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Yuqing Chen & Bo Zhang

  2. Tianjin Key Laboratory of Marine Resources and Chemistry, College of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Bo Zhang

Authors
  1. Yuqing Chen
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  2. Bo Zhang
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Correspondence to Bo Zhang .

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Editors and Affiliations

  1. College of Bioengineering ,Tianjin University of Science and Technology, Tianjin, People's Republic of China

    Tong-Cun Zhang

  2. Nanjing University of Technology, Nanjing, People's Republic of China

    Pingkai Ouyang

  3. Department of Microbiology & Molecular Genetics, University of Texas- Houston Medical School, Texas, Texas, USA

    Samuel Kaplan

  4. Wellcome Trust Sanger Institute, Cambridge, United Kingdom

    Bill Skarnes

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Chen, Y., Zhang, B. (2014). Different Chemicals Stimulate Diapause Termination of Artemia Embryos. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 250. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37922-2_107

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  • DOI: https://doi.org/10.1007/978-3-642-37922-2_107

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-37921-5

  • Online ISBN: 978-3-642-37922-2

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