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Effects of Environmental Conditions on Synthesis of the Mycosporine-Like Amino Acid in Nostoc flagelliforme Cells

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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 249))

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Abstract

Nostoc flagelliforme is a terrestrial cyanobacterium distributed in arid or semiarid area, which has the capability of nitrogen-fixing and photosynthesis. The multifunction of MAAs is of great significance for N. flagelliforme to adapt to abiotic stresses. The changes of mycosporine-like amino acids in N. flagelliforme cells with UV-B radiation and salt stress were studied. The results showed that MAAs concentrations reached 32.26 mg/g after 12 h under UV-B radiation (5 W/m2). After 2 days cultivation, MAAs synthesis was up to the maximum with the BG11 medium containing 50 mM NaCl and 50 mM NH4Cl, and their concentrations were 45.09 and 35.14 mg/g respectively. HPLC analysis revealed the control sample contained nine MAAs. Ten MAAs were observed after UV-B treatment, and two new MAA compounds were found but one MAA disappeared. With the treatment of NaCl and NH4Cl for 2 days, only seven MAAs were discovered and no new MAAs appeared.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 20806047; 20776112), China Postdoctoral Science Foundation (No. 20090450116) and Excellent Middle Aged and Young Scientist Award Foundation of Shandong Province (No. BS2011SW029).

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Authors and Affiliations

  1. Shandong provincial key laboratory of Microbial Engineeringy, Qilu University of Technology, 250353, Jinan, People’s Republic of China

    Rong Liu, Haifeng Yu & Yuxia Sa

Authors
  1. Rong Liu
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  2. Haifeng Yu
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  3. Yuxia Sa
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Corresponding author

Correspondence to Haifeng Yu .

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Editors and Affiliations

  1. College of Bioengineering, Tianjin University of Science and Technology, Tianjin, People's Republic of China

    Tong-Cun Zhang

  2. Nanjing University of Technology, Nanjing, People's Republic of China

    Pingkai Ouyang

  3. Department of Microbiology & Molecular Genetics, University of Texas Houston Medical School, Texas, Texas, USA

    Samuel Kaplan

  4. Wellcome Trust Sanger Institute, Cambridge, United Kingdom

    Bill Skarnes

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Liu, R., Yu, H., Sa, Y. (2014). Effects of Environmental Conditions on Synthesis of the Mycosporine-Like Amino Acid in Nostoc flagelliforme Cells. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_9

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  • DOI: https://doi.org/10.1007/978-3-642-37916-1_9

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-37915-4

  • Online ISBN: 978-3-642-37916-1

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