Abstract
The content of trehalose is widely believed to be a major determinant of stress resistance in Saccharomyces cerevisiae. A neutral trehalase gene, NTH1, is involved in trehalose degradation and TPS1 encoding trehalose biosynthesis enzyme is improtant to trehalose accumulation in S. cerevisiae. In this research, the responses of two engineering strains, the deletion of NTH1 (Δnth1) and overexpression TPS1 (Δnth1 + TPS1), were investigated to freezing stresses. High trehalose accumulation and growth activity were observed in Δnth1 + TPS1 strain after freezing stress induction. Our results indicated that high trehalose accumulation can make yeast cells resistant freezing stress.
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Acknowledgments
The current study was financially supported by the National Natural Science Foundation of China (31171730), program for Changjiang Scholars and Innovative Research Team in University (IRT1166), and Major Project of Research Program on Applied Fundamentals and Advanced Technologies of Tianjin (10JCZDJC16700).
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Wu, M., Zhang, C., Sun, X., Wang, G., Liu, Y., Xiao, D. (2014). Effects of NTH1 Gene Deletion and Overexpressing TPS1 Gene on Freeze Tolerance in Baker’s Yeast. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_46
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DOI: https://doi.org/10.1007/978-3-642-37916-1_46
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