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Clone and Expression of High Yield Recombinant Trehalose Synthase in Bacillus subtilis

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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 249))

Abstract

Trehalose synthase is one kind of intermolecular transglucosylation enzyme, which catalyzes the conversion of maltose to trehalose. In this study the trehalose synthase gene was amplified from Pseudomonas putida P06 genomic DNA, ligated with pMA5 vector, cloned into Bacillus subtilis WB800 and had good expression with the molecular weight of 77 KD. The recombinant trehalose synthase expression conditions were performed and enzyme reaction key parameters were investigated. The results showed the enzyme had optimal activity when it reacted for 2 h at 35 °C with pH value of 7.5 and the substrate concentration was 30 %. Trehalose content of samples was detected by HPLC and the enzyme activity reached to 318.12 U/ml in crude enzyme solution. This study is the first report about the expression of trehalose synthase in Bacillus subtilis, which lays the basis for trehalose large scale industrial production.

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Authors and Affiliations

  1. School of Food and Bioengineering, Qilu University of Technology, Jinan, 250300, China

    Jing Su, Chunling Ma, Tengfei Wang, Piwu Li & Ruiming Wang

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  1. Jing Su
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  2. Chunling Ma
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  3. Tengfei Wang
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  4. Piwu Li
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  5. Ruiming Wang
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Correspondence to Ruiming Wang .

Editor information

Editors and Affiliations

  1. College of Bioengineering, Tianjin University of Science and Technology, Tianjin, People's Republic of China

    Tong-Cun Zhang

  2. Nanjing University of Technology, Nanjing, People's Republic of China

    Pingkai Ouyang

  3. Department of Microbiology & Molecular Genetics, University of Texas Houston Medical School, Texas, Texas, USA

    Samuel Kaplan

  4. Wellcome Trust Sanger Institute, Cambridge, United Kingdom

    Bill Skarnes

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Su, J., Ma, C., Wang, T., Li, P., Wang, R. (2014). Clone and Expression of High Yield Recombinant Trehalose Synthase in Bacillus subtilis . In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_4

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  • DOI: https://doi.org/10.1007/978-3-642-37916-1_4

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-37915-4

  • Online ISBN: 978-3-642-37916-1

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