Abstract
The gene recG encodes DNA helicase RecG which is involved in DNA replication, recombination, and repair. In this research, the λ Red homologous recombination system was used to delete recG gene of Escherichia coli resulting in the cut-off of part of the DNA repair pathway. The results showed that the recG-deficient mutant strain showed increasing significant growth disadvantages compared to the wild-type strain in the presence of acetic acid varied from 0.02–0.09 %. It was also observed that over 10-fold more recG-deficient mutant cells died than that of wild-type cells after shocking with 0.5 % acetic acid for 40 mins, specially, the mutant could not grow under 0.07 % acetic acid condition compared with the wild-type strain. The results showed that recG-deficient mutant strain is more susceptible to high concentration of acetic acid than the wild-type strain, indicating RecG may involve the repair of DNA damage caused by acetic acid.
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Acknowledgments
This work was supported by National High Technology Research and Development Program of China (2012AA022108), Program for Changjiang Scholars and Innovative Research Team in University (IRT1166), Doctoral Program Foundation of Institutions of Higher Education of China (20101208120003), National Natural Science Foundation of China (31201406), and Foundation of Tianjin University of Science and Technology (20110114).
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Zheng, Y., Han, Q., Jiang, C., Nie, Z., Wang, M. (2014). Deletion of Gene recG and its Susceptibility to Acetic Acid in Escherichia coli . In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_36
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DOI: https://doi.org/10.1007/978-3-642-37916-1_36
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