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Effect of Gene FPS1 on Accumulation of Glycerol in Zygosaccharomyces rouxii

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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 249))

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Abstract

Zygosaccharomyces rouxii was applied in high-salt liquid state fermentation of soy sauce. The gene FPS1 encoding Fps1p was analyzed in the Z. rouxii in order to investigate the effect of gene FPS1 on glycerol accumulation. The sequence result showed that FPS1 had an open reading frame of 2079 bp. It was found that the expression level of FPS1 in the Z. rouxii decreased with the increase of osmotic stress. Furthermore, overexpression of FPS1 could depress the salt-tolerance compared to the control.

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Acknowledgments

This work was supported by these projects in China (2013AA102106, 2012BAD33B04, 31371819, 2012GB2A100016, 2012AA022108, 31171731, 10ZCZDSY07000, 2012AA021303 and IRT1166).

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Authors and Affiliations

  1. Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Ministry of Education, Tianjin, 300457, People’s Republic of China

    Yonghua Wei, Cong Wang, Xiaohong Cao & Lihua Hou

  2. School of Biological Sciences and Engineering, Shaanxi University of Technology, Hanzhong, 723001, People’s Republic of China

    Yonghua Wei

Authors
  1. Yonghua Wei
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  2. Cong Wang
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  3. Xiaohong Cao
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  4. Lihua Hou
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Corresponding author

Correspondence to Lihua Hou .

Editor information

Editors and Affiliations

  1. College of Bioengineering, Tianjin University of Science and Technology, Tianjin, People's Republic of China

    Tong-Cun Zhang

  2. Nanjing University of Technology, Nanjing, People's Republic of China

    Pingkai Ouyang

  3. Department of Microbiology & Molecular Genetics, University of Texas Houston Medical School, Texas, Texas, USA

    Samuel Kaplan

  4. Wellcome Trust Sanger Institute, Cambridge, United Kingdom

    Bill Skarnes

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© 2014 Springer-Verlag Berlin Heidelberg

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Wei, Y., Wang, C., Cao, X., Hou, L. (2014). Effect of Gene FPS1 on Accumulation of Glycerol in Zygosaccharomyces rouxii . In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_26

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  • DOI: https://doi.org/10.1007/978-3-642-37916-1_26

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-37915-4

  • Online ISBN: 978-3-642-37916-1

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