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Characterization of a 2,3-Butanediol Producing Bacterial Strain and Optimization of Fermentation Medium

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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 249))

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Abstract

A bacterial strain G12 capable of producing high content of 2,3- butanediol (2,3-BD, BD) was isolated from raw milk samples. With the analysis of its 16S rRNA gene sequence, strain G12 was identified as Serratia marcescens. Carbon source evaluation experiments showed that with glycerol as carbon source, strain G12 produced relatively high amount of 2,3-BD and the lowest amount of byproduct acetoin among the tested carbon sources. Furthermore, the optimal fermentation medium was determined by single factor experiments and orthogonal experimental design, and it was composed of glycerin 130 g/L, peptone 16 g/L, yeast extract 5 g/L, K2HPO4 1 g/L, and MnSO4·7H2O 0.025 g/L. Confirmation experiments were also performed. The final yield of 2,3-BD in the shaking-flask was up to 37.400 g/L, and the substrate conversion rate was 79.62 % of the theoretical value.

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Acknowledgments

This work was partly supported by The National Natural Science Foundation of China (Grant No. 30970114) and The National Key Technology R&D Program of China (Grant No. 2011BAC11B05).

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Authors and Affiliations

  1. Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China

    Songsong Gao, Hongjiang Yang & Xuying Qin

Authors
  1. Songsong Gao
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  2. Hongjiang Yang
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  3. Xuying Qin
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Corresponding author

Correspondence to Hongjiang Yang .

Editor information

Editors and Affiliations

  1. College of Bioengineering, Tianjin University of Science and Technology, Tianjin, People's Republic of China

    Tong-Cun Zhang

  2. Nanjing University of Technology, Nanjing, People's Republic of China

    Pingkai Ouyang

  3. Department of Microbiology & Molecular Genetics, University of Texas Houston Medical School, Texas, Texas, USA

    Samuel Kaplan

  4. Wellcome Trust Sanger Institute, Cambridge, United Kingdom

    Bill Skarnes

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Gao, S., Yang, H., Qin, X. (2014). Characterization of a 2,3-Butanediol Producing Bacterial Strain and Optimization of Fermentation Medium. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_17

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  • DOI: https://doi.org/10.1007/978-3-642-37916-1_17

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-37915-4

  • Online ISBN: 978-3-642-37916-1

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