Abstract
With the increase of the proportion of beer adjunct, the concentration of higher alcohols in the final product is high, resulting to a heavier beer flavor. In our previous work, we constructed a LEU2-deleted strain S6-2 to reduce the higher alcohol production. This study focuses to further reduce the production of higher alcohols, especially isoamyl alcohol, in high adjunct beer via disrupting a second copy of LEU2 gene in the host strain S6-2. We constructed a recombined plasmid vector pUC-LBKA, from which the LA-KanMX-LB cassette was cloned through PCR amplification. The cassette was subsequently transformed into S6-2, creating the mutant strain S6-3 with disruptions of two LEU2 gene copies. Finally, we examined the β-isopropylmalate dehydrogenase (β-IPM) activity and the production of higher alcohol. Our results indicate that LEU2 deletion can significantly reduce the β-IPM activity as well as the total higher alcohol production in high adjunct beer.
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Acknowledgments
This work was financially supported by the program of National High Technology Research and Development Program of China (863 Program) (Grant No. SS2012AA023408), the Cheung Kong Scholars and Innovative Research Team Program in University of Ministry of Education, China (Grant No. IRT1166), and Application Base and Frontier Technology Project of Tianjin, China (Grant No. 09JCZDJC17900).
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Liu, Y., Dong, J., Chen, Y., Wu, M., Peng, X., Xiao, D. (2014). Effect of LEU2 Gene Deletion on Higher Alcohols Production of High Adjunct Beer. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_13
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DOI: https://doi.org/10.1007/978-3-642-37916-1_13
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