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The Saccharification Step: Trichoderma Reesei Cellulase Hyper Producer Strains

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Lignocellulose Conversion

Abstract

One of the major applications of cellulases is to produce fermentable sugars from lignocellulosic biomass for biofuels production. The filamentous fungus Trichoderma reesei is known to be hyper producer of cellulases and hemicellulases and it is widely used for commercial scale production of these enzymes using novel fermentation techniques. Some of the T. reesei industrial strains produce over 100 g/l of cellulases. However, there are still technical and economic constraints to the development of cheap commercial cellulase production process. Here, we bring together and discuss the results on T. reesei as cellulase producer, the different kinds of enzymes it expresses, recent genomic, genetic, and metabolic engineering approaches that have helped to improve the biomass degrading enzyme mixture and the strategies adopted to reduce the cost of enzymes during fermentation process. Current efforts and some future perspectives for reducing the cost of enzymes by using cheaper substrates, recycling enzyme during the hydrolysis and fermentation process, and on-site enzyme production in the biorefinery facility are also discussed.

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Acknowledgments

This work was supported by U.S. Department of Energy through the DOE Great Lakes Bioenergy Research Center (GLBRC) Grant DE‐FC02‐07ER64494. We would like to thank James Humpula from Biomass conversion research laboratory located at Michigan State University, East Lansing for giving his valuable suggestion while drafting this chapter.

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  1. Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, Lansing, MI, 48823, USA

    Venkatesh Balan, Mingjie Jin, Alan Culbertson & Nirmal Uppugundla

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  1. Complesso Universitario Monte S. Angelo, Dept. of Chemical Sciences, University of Naples "Federico II", Via Cintia 4, Naples, Italy

    Vincenza Faraco

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Balan, V., Jin, M., Culbertson, A., Uppugundla, N. (2013). The Saccharification Step: Trichoderma Reesei Cellulase Hyper Producer Strains. In: Faraco, V. (eds) Lignocellulose Conversion. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37861-4_4

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