Preparation and Application of Low Molecular Weight Sodium Hyaluronate

Abstract

In this study, sodium hyaluronate was hydrolyzed in order to produce low molecular weight sodium hyaluronate and acetylated low molecular weight sodium. The sodium hyaluronate produced in this study had the average molecular weight that ranged from several hundreds of thousands to 3,500 Da by adjusting the acid concentration and reaction time, and then the sodium hyaluronate was converted to acetylated low molecular weight sodium hyaluronate. The testing on cytotoxicity in HaCaT Human Skin Keratinocytes, all the samples, which were processed with the low molecular weight sodium hyaluronate and acetylated low molecular weight sodium, appeared to have no cytotoxicity. The testing result on anti-wrinkle activity in CCD 986-sk human dermal fibroblasts showed that as the molecular weight low molecular weight sodium hyaluronate reduced, the effects on anti-wrinkle activity was getting better, and increase in the amount of PIP synthesis at the LMHA-5, LMHA-6, which were the acetylated samples, had an excellent effect on anti-wrinkle activity. And the measurement results of anti-inflammation showed that as the molecular weight reduced, the amount of NO generated was getting smaller, and the acetylated samples appeared to have much smaller amount of NO, so that acetylated low molecular weight sodium hyaluronate could be considered to have an excellent anti-inflammation effect.