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Qualitative and Quantitative Analysis: Interpretation of Electropherograms

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Book cover Electromigration Techniques

Part of the book series: Springer Series in Chemical Physics ((CHEMICAL,volume 105))

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Abstract

In this chapter the basic information on qualitative and quantitative analysis in CE is provided. Migration time and spectral data are described as the most important parameters used for identification of compounds. The parameters that negatively influence qualitative analysis are briefly mentioned. In the quantitative analysis section the external standard and internal standard calibration methods are described. Variables influencing peak height and peak area in capillary electrophoresis are briefly summarized. Also, a discussion on electrodisperssion and its influence on a observed peak shape is provided.

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References

  1. H.J. Kuss, S. Kromidas (eds.), Quantification in LC and GC (Wiley, Weinheim, 2009)

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  3. D.R. Baker, Capillary Electrophoresis. Techniques in Analytical Chemistry (Wiley, New York, 1995)

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  4. D. Heiger, R. Weinberger (1994) Determination of small ions by capillary electrophoresis with indirect photometric detection. Hewlett-Packard Application Note

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  5. P. Gebauer, P. Bocek, Predicting peak symmetry in capillary zone electrophoresis: the concept of the peak shape diagram. Anal. Chem. 69(8), 1557–1563 (1997)

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Author information

Authors and Affiliations

  1. Faculty of Chemistry, Nicolaus Copernicus University, Toruń, Poland

    Michał Szumski & Bogusław Buszewski

Authors
  1. Michał Szumski
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  2. Bogusław Buszewski
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Corresponding author

Correspondence to Michał Szumski .

Editor information

Editors and Affiliations

  1. Fac. Chemistry, Dept. Environmental Chemistry &, Nicolaus Copernicus University, Gagarin 7, Torun, 87-100, Poland

    Boguslaw Buszewski

  2. Faculty of Chemistry, Department of Environmental Chemistry, Nicolaus Copernicus University, Gagarin 7, Toruń, 87-100, Poland

    Ewelina Dziubakiewicz

  3. Faculty of Chemistry, Department of Environmental Chemistry, Nicolaus Copernicus University, Gagarin 7, Torun, 87-100, Poland

    Michal Szumski

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Szumski, M., Buszewski, B. (2013). Qualitative and Quantitative Analysis: Interpretation of Electropherograms. In: Buszewski, B., Dziubakiewicz, E., Szumski, M. (eds) Electromigration Techniques. Springer Series in Chemical Physics, vol 105. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35043-6_4

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  • DOI: https://doi.org/10.1007/978-3-642-35043-6_4

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-35042-9

  • Online ISBN: 978-3-642-35043-6

  • eBook Packages: Physics and AstronomyPhysics and Astronomy (R0)

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