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Methods of Analyte Concentration in a Capillary

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Electromigration Techniques

Part of the book series: Springer Series in Chemical Physics ((CHEMICAL,volume 105))

Abstract

Online sample concentration techniques in capillary electrophoresis separations have rapidly grown in popularity over the past few years. During the concentration process, diluted analytes in long injected sample are concentrated into a short zone, then the analytes are separated and detected. A large number of contributions have been published on this subject proposing many names for procedures utilizing the same concentration principles. This chapter brings a unified view on concentration, describes the basic principles utilized, and shows a list of recognized current operational procedures. Several online concentration methods based on velocity gradient techniques are described, in which the electrophoretic velocities of the analyte molecules are manipulated by field amplification, sweeping and isotachophoretic migration, resulting in the online concentration of the analyte.

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  1. Faculty of Chemistry, University of Łódź, Łódź, Poland

    Paweł Kubalczyk & Edward Bald

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  1. Paweł Kubalczyk
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  2. Edward Bald
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Correspondence to Paweł Kubalczyk .

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  1. Fac. Chemistry, Dept. Environmental Chemistry &, Nicolaus Copernicus University, Gagarin 7, Torun, 87-100, Poland

    Boguslaw Buszewski

  2. Faculty of Chemistry, Department of Environmental Chemistry, Nicolaus Copernicus University, Gagarin 7, Toruń, 87-100, Poland

    Ewelina Dziubakiewicz

  3. Faculty of Chemistry, Department of Environmental Chemistry, Nicolaus Copernicus University, Gagarin 7, Torun, 87-100, Poland

    Michal Szumski

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Kubalczyk, P., Bald, E. (2013). Methods of Analyte Concentration in a Capillary. In: Buszewski, B., Dziubakiewicz, E., Szumski, M. (eds) Electromigration Techniques. Springer Series in Chemical Physics, vol 105. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35043-6_12

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