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Special Ancillary Techniques: Immunohistochemistry

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Abstract

The role of immunohistochemistry in the assessment of breast lesions, in particular breast cancers, cannot be overemphasized. Currently, there is a whole gamut of markers that are being used, aiding in the differentiation between benign and malignant breast lesions, prognostication of breast cancers, and prediction of responses to therapy. While most of the routine immunohistochemistry studies are being done on the paraffin-embedded histologic samples using either the resection specimen or the preoperative biopsy, the use of cytologic preparations in immunohistochemistry is gaining popularity and experience.

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References

  • Aiad HA, Abd El-Halim Kandil M, Abd El-Wahed MM et al (2011) Diagnostic role of p63 immunostaining in fine needle aspiration cytology of different breast lesions. Acta Cytol 55:149–157

    Article  PubMed  CAS  Google Scholar 

  • Becette V, Lerebours F, Spyratos F et al (2011) Cell immunomarker studies of fine-needle cytopuncture blocks for tumor response prediction after preoperative chemotherapy and prognosis in operable nonmetastatic primary breast carcinoma. Breast J 17:121–128

    Article  PubMed  Google Scholar 

  • Briffod M, Hacène K, Le Doussal V (2000) Immu­nohistochemistry on cell blocks from fine-needle cytopunctures of primary breast carcinomas and lymph node metastases. Mod Pathol 13:841–850

    Article  PubMed  CAS  Google Scholar 

  • Cano G, Milanezi F, Leitão D et al (2003) Estimation of hormone receptor status in fine-needle aspirates and paraffin-embedded sections from breast cancer using the novel rabbit monoclonal antibodies SP1 and SP2. Diagn Cytopathol 29:207–211

    Article  PubMed  CAS  Google Scholar 

  • Colecchia M, Frigo B, Zucchi A et al (1995) p53 protein expression in fine-needle aspirates of breast cancer: an immunocytochemical assay for identifying high-grade ductal carcinomas. Diagn Cytopathol 13:128–132

    Article  PubMed  CAS  Google Scholar 

  • Corkill ME, Katz R (1994) Immunocytochemical staining of c-erb B-2 oncogene in fine-needle aspirates of breast carcinoma: a comparison with tissue sections and other breast cancer prognostic factors. Diagn Cytopathol 11:250–254

    Article  PubMed  CAS  Google Scholar 

  • Dalquen P, Baschiera B, Chaffard R et al (1997) MIB-1 (Ki-67) immunostaining of breast cancer cells in cytologic smears. Acta Cytol 41:229–237

    Article  PubMed  CAS  Google Scholar 

  • Delgallo WD, Rodrigues JR, Bueno SP et al (2010) Cell blocks allow reliable evaluation of expression of basal (CK5/6) and luminal (CK8/18) cytokeratins and smooth muscle actin (SMA) in breast carcinoma. Cytopathology 21:259–266

    Article  PubMed  CAS  Google Scholar 

  • Dowsett M, Nielsen TO, A’Herm R et al (2011) Assessment of Ki67 in breast cancer: recommendations from the International Ki67 in Breast Cancer working group. J Natl Cancer Inst 103:1656–1664

    Article  PubMed  CAS  Google Scholar 

  • Gusterson BA, Gullick WJ, Venter DJ et al (1988) Immunohistochemical localization of c-erbB-2 in human breast carcinomas. Mol Cell Probes 2:383–391

    Google Scholar 

  • Hanley KZ, Birdsong GG, Cohen C et al (2009) Immunohistochemical detection of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 expression in breast carcinomas: comparison on cell block, needle-core, and tissue block preparations. Cancer 117:279–288

    PubMed  Google Scholar 

  • Harton AM, Wang HH, Schnitt SJ et al (2007) p63 immunocytochemistry improves accuracy of diagnosis with fine-needle aspiration of the breast. Am J Clin Pathol 128:80–85

    Article  PubMed  Google Scholar 

  • Jares P, Rey MJ, Fernández PL et al (1997) Cyclin D1 and retinoblastoma gene expression in human breast carcinoma: correlation with tumour proliferation and oestrogen receptor status. J Pathol 182:160–166

    Article  PubMed  CAS  Google Scholar 

  • Jayaram G, Elsayed EM (2005) Cytologic evaluation of prognostic markers in breast carcinoma. Acta Cytol 49:605–610

    Article  PubMed  Google Scholar 

  • Jorda M, Ganjei P, Nadji M (1994) Retrospective c-erbB-2 immunostaining in aspiration cytology of breast cancer. Diagn Cytopathol 11:262–265

    Article  PubMed  CAS  Google Scholar 

  • Kalogeraki A, Garbagnati F, Santinami M et al (2003) E-cadherin expression on fine needle aspiration biopsies of breast invasive ductal carcinomas and its relationship to clinicopathologic factors. Acta Cytol 47:363–367

    Article  PubMed  Google Scholar 

  • Klorin G, Keren R (2003) Prognostic markers in histologic and cytologic specimens of breast cancer. Anal Quant Cytol Histol 25:297–302

    PubMed  Google Scholar 

  • Koutselini H, Malliri A, Field JK et al (1991) p53 expression in cytologic specimens from benign and malignant breast lesions. Anticancer Res 11:1415–1420

    PubMed  CAS  Google Scholar 

  • Lofgren L, Skoog L, von Schoultz E et al (2003) Hormone receptor status in breast cancer--a comparison between surgical specimens and fine needle aspiration biopsies. Cytopathology 14:136–142

    Article  PubMed  CAS  Google Scholar 

  • Makris A, Allred DC, Powles TJ et al (1997) Cytological evaluation of biological prognostic markers from primary breast carcinomas. Breast Cancer Res Treat 44:65–74

    Article  PubMed  CAS  Google Scholar 

  • Marrazzo A, Taormina P, Leonardi P et al (1995) Immunocytochemical determination of estrogen and progesterone receptors on 219 fine-needle aspirates of breast cancer. A prospective study. Anticancer Res 15:521–526

    PubMed  CAS  Google Scholar 

  • Midulla C, Pisani T, De Lorio P et al (2002) Cytological analysis and immunocytochemical expression of Ki67 and Bcl-2 in breast proliferative lesions. Anticancer Res 22:1341–1345

    PubMed  CAS  Google Scholar 

  • Moriki T, Takahashi T, Ueta S et al (2004) Hormone receptor status and HER2/neu overexpression determined by automated immunostainer on routinely fixed cytologic specimens from breast carcinoma: correlation with histologic sections determinations and diagnostic pitfalls. Diagn Cytopathol 30:251–256

    Article  PubMed  Google Scholar 

  • Nizzoli R, Bozzetti C, Savoldi L et al (1994) Immunocytochemical assay of estrogen and progesterone receptors in fine needle aspirates from breast cancer patients. Acta Cytol 38:933–938

    PubMed  CAS  Google Scholar 

  • Nizzoli R, Bozzetti C, Naldi N et al (2000) Comparison of the results of immunocytochemical assays for biologic variables on preoperative fine-needle aspirates and on surgical specimens of primary breast carcinomas. Cancer 90:61–66

    Article  PubMed  CAS  Google Scholar 

  • Nizzoli R, Bozzetti C, Crafa P et al (2003) Immuno­cytochemical evaluation of HER-2/neu on fine-needle aspirates from primary breast carcinomas. Diagn Cytopathol 28:142–146

    Article  PubMed  Google Scholar 

  • Ostrowski ML, Pindur L, Laucirica R et al (2001) Proliferative activity in invasive breast carcinoma: a comprehensive comparison of MIB-1 immunocytochemical staining in aspiration biopsies to image analytic, flow cytometric and histologic parameters. Acta Cytol 45:965–972

    Article  PubMed  CAS  Google Scholar 

  • Park K, Han S, Kim HY et al (2001) Cytologic evaluation of cyclin D1 expression in primary breast carcinoma. Cancer 93:211–215

    Article  PubMed  CAS  Google Scholar 

  • Pelosi G, Bresaola E, Rodella S et al (1994) Expression of proliferating cell nuclear antigen, Ki-67 antigen, estrogen receptor protein, and tumor suppressor p53 gene in cytologic samples of breast cancer: an immunochemical study with clinical, pathobiological, and histologic correlations. Diagn Cytopathol 11:131–140

    Article  PubMed  CAS  Google Scholar 

  • Perou CM, Sørlie T, Eisen MB et al (2000) Molecular portraits of human breast tumours. Nature 406:747–752

    Article  PubMed  CAS  Google Scholar 

  • Railo M, Nordling S, Krogerus L et al (1996) Preoperative assessment of proliferative activity and hormonal receptor status in carcinoma of the breast: a comparison of needle aspiration and needle-core biopsies to the surgical specimen. Diagn Cytopathol 15:205–210

    Article  PubMed  CAS  Google Scholar 

  • Reiner A, Reiner G, Spona J et al (1987) Estrogen receptor immunocytochemistry for preoperative determination of estrogen receptor status on fine-needle aspirates of breast cancer. Am J Clin Pathol 88:399–404

    PubMed  CAS  Google Scholar 

  • Reis-Filho JS, Milanezi F, Amendoeira I et al (2002) p63 staining of myoepithelial cells in breast fine needle aspirates: a study of its role in differentiating in situ from invasive ductal carcinomas of the breast. J Clin Pathol 55:936–939

    Article  PubMed  CAS  Google Scholar 

  • Schnitt SJ (2010) Classification and prognosis of invasive breast cancer: from morphology to molecular taxonomy. Mod Pathol 23(Suppl 2):S60–S64

    Article  PubMed  Google Scholar 

  • Shabaik A, Lin G, Peterson M et al (2011) Reliability of Her2/neu, estrogen receptor, and progesterone receptor testing by immunohistochemistry on cell block of FNA and serous effusions from patients with primary and metastatic breast carcinoma. Diagn Cytopathol 39:328–332

    Article  PubMed  Google Scholar 

  • Slamon DJ, Godolphin W, Jones LA et al (1989) Studies of the HER-2/neu proto-oncogene in human breast and ovarian cancer. Science 244:707–712

    Article  PubMed  CAS  Google Scholar 

  • Stephenson TJ, Royds JA, Silcocks PB et al (1994) Diagnostic associations of p53 immunostaining in fine needle aspiration cytology of the breast. Cytopathology 5:146–153

    Article  PubMed  CAS  Google Scholar 

  • Tafjord S, Bøhler PJ, Risberg B et al (2002) Estrogen and progesterone hormone receptor status in breast carcinoma: comparison of immunocytochemistry and immunohistochemistry. Diagn Cytopathol 26:137–141

    Article  PubMed  Google Scholar 

  • Williams SL, Birdsong GG, Cohen C et al (2009) Immunohistochemical detection of estrogen and progesterone receptor and HER2 expression in breast carcinomas: comparison of cell block and tissue block preparations. Int J Clin Exp Pathol 2:476–480

    PubMed  CAS  Google Scholar 

  • Zoppi J, Rotundo AV, Sundblad AS (2002) Correlation of immunocytochemical and immunohistochemical determination of estrogen and progesterone receptors in breast cancer. Acta Cytol 46:337–340

    Article  PubMed  Google Scholar 

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Tse, G., Tan, P.H., Schmitt, F. (2013). Special Ancillary Techniques: Immunohistochemistry. In: Fine Needle Aspiration Cytology of the Breast. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35000-9_14

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  • DOI: https://doi.org/10.1007/978-3-642-35000-9_14

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-34999-7

  • Online ISBN: 978-3-642-35000-9

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