Zusammenfassung
DNA aufzuspüren, das heißt zumeist: Hybridisieren. Wie man DNA und RNA auf eine Membran transferiert, ist bereits in Abschn. 3.3 erklärt worden, jetzt werden wir uns damit beschäftigen, was man damit anschließend anstellt.
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Notes
- 1.
Eine Alternative zur PCR, bei der die Templatemenge sogar quantifiziert werden kann, ist der enzymatische Nachweis von DNA, Abschn. 2.5.
- 2.
[Na+] = Natriumkonzentration in mol/l, % GC = Anteil von Guanin und Cytosin, L = Länge der Sonde in Basen (N. B. log[Na+] ergibt üblicherweise einen negativen Wert, dadurch verwandelt sich „−16,6“ in „+16,6“).
- 3.
Ist es nicht erstaunlich, dass man 2007, also sechs Jahre nach der Erstpublikation des menschlichen Genoms, immer noch nicht wusste, wie viele Gene dieses Genom enthält?!
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Mülhardt, C. (2013). Wie man DNA aufspürt. In: Der Experimentator Molekularbiologie/Genomics. Der Experimentator. Springer Spektrum, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-34636-1_7
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