Abstract
If there are indications for the involvement of a molecule or protein in the process of calcium signalling (e.g., from patch-clamp recordings as stated above), there are many techniques that can be used to identify an unknown player. This biochemical and molecular biology-based methodology is reviewed in other sources. Examples include high-pressure liquid chromatography (HPLC), 2D-gel electrophoresis, mass spectrometry, matrix-assisted laser desorption/ionisation in combination with time-of-flight mass spectrometer (MALDI-TOF) and expression analysis with DNA chips or next-generation sequencing.
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© 2013 Springer-Verlag Berlin Heidelberg
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Kaestner, L. (2013). Molecular biology based approaches. In: Calcium signalling. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-34617-0_6
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DOI: https://doi.org/10.1007/978-3-642-34617-0_6
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Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-34616-3
Online ISBN: 978-3-642-34617-0
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