Abstract
The aim of this study was to evaluate impact of transplantation of bone marrow mesenchymal stromal cells on recovery after polytrauma and bone fracture repair.
27 Wistar-Kyoto rats were divided into 3 groups (n=9): normal control (A), polytrauma (B), and polytrauma treated with BM MMSCs transplantation (C). The experimental polytrauma model was made on male rats by causing multiple fractures and hemorrhagic shock. At 36 hours and nine days after surgery 9 rats received syngeneic BM MMSCs (1x106 cells/kg) intravenously. In 30 days post-surgery period changes in animals’ body temperature, weight and locomotor behavior as well as blood parameters were recorded. At day 30 rats were euthanized and macroscopic and histological observations of rats lower extremities was performed.
The treated animals showed faster weight gain, as well as regaining their physical activity earlier. These outcomes were associated with locomotor activity test results, blood glucose and lactate ratios and less marked muscle atrophy.
Rat treatment with BM MMSCs transplantation stimulated bone fracture healing - bone edge consolidation and enhanced callus formation, as well as the size and maturity of newly formed trabeculae.
Red blood analysis results showed delayed recovery after hemorrhage in the rats receiving BM MMSCs: restoration of RBC counts, hematocrit and HGB level velocity was less than in the untreated animals.
BM MMSCs transplantation improved rats rehabilitation scores after experimental polytrauma.
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Krumina, G. et al. (2013). Bone Marrow Multipotent Mesenchymal Stromal Cells Transplantation Effects after Experimental Polytrauma in Rats. In: Dekhtyar, Y., Katashev, A., Lancere, L. (eds) International Symposium on Biomedical Engineering and Medical Physics, 10-12 October, 2012, Riga, Latvia. IFMBE Proceedings, vol 38. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-34197-7_53
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DOI: https://doi.org/10.1007/978-3-642-34197-7_53
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-34196-0
Online ISBN: 978-3-642-34197-7
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