Abstract
The mazEF locus is one of the most extensively characterized toxin-antitoxin (TA) systems. MazF is an endoribonuclease that cleaves RNA at a specific sequence. MazF is conserved in most bacterial and some archaeal species. Since the discovery of MazF in Escherichia coli, a number of MazF homologues and other mRNA interferases with different mRNA cleavage specificities have been elucidated. Here we describe their unique biochemical features, the regulatory mechanisms of the MazF activity and presumed physiological roles of MazF in the cells.
Keywords
- Cleavage Site
- Amino Acid Starvation
- Cellular mRNAs
- Primer Extension Experiment
- Extracellular Death Factor
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Acknowledgments
We would like to dedicate this chapter for late Dr. Tsutomu Shimazu, who demonstrated for the first time the MazF toxicity in mammalian cells (Shimazu et al. 2007). We also thank to Dr. Vikas Nanda, Mr. Jung-Ho Park, and Ms. Sehrish Ajimal for their critical reading of this chapter. This work was supported by an NIH grant, 1RO1GM081567 and 3RO1GM081567-02S1.
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Yamaguchi, Y., Inouye, M. (2013). Type II Toxin-Antitoxin Loci: The mazEF Family. In: Gerdes, K. (eds) Prokaryotic Toxin-Antitoxins. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-33253-1_7
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