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Interaction Between Free Radicals and Antioxidants in Human Skin

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Non Invasive Diagnostic Techniques in Clinical Dermatology

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Abstract

Free radicals are produced in the human body as a result of the metabolism [1, 2]. They are important for signalling processes in the organism [1, 3] as well as disinfector against viruses and bacteria [4]. If their concentration exceeds a critical value, these highly reactive molecules can destroy cells and cell compartments and cause serious damage to the human body [5, 6]. An enhanced radical formation is due to inflammatory processes in the human body or to environmental influences [7, 8]. In this context, high doses of solar radiation are dangerous [9]. Enhanced UV doses do not only evoke sunburns and skin aging but induce even skin cancer if significant amounts of free radicals are generated in the skin [10]. With the antioxidant protection system, the human organism has developed a defense mechanism against the destructive effect of the free radicals. The most important antioxidants in this defense system are the vitamins, the carotenoids, and some enzymes [11, 12]. The antioxidants are capable of neutralizing the free radicals before the same start damaging the body. Most of these antioxidants cannot be generated by the human body automatically, but must be taken in with food rich in fruit and vegetables. If the free radicals exceed a critical level, the antioxidants are destroyed, too. Thus, the detection of antioxidants in tissue permits at the same time conclusions to be drawn about the formation of radicals [13].

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References

  1. Droge W (2002) Free radicals in the physiological control of cell function. Physiol Rev 82:47–95

    PubMed  CAS  Google Scholar 

  2. Iannone A, Marconi A, Zambruno G, Giannetti A, Vannini V, Tomasi A (1993) Free radical production during metabolism of organic hydroperoxides by normal human keratinocytes. J Invest Dermatol 101:59–63

    Article  PubMed  CAS  Google Scholar 

  3. Jackson MJ (1999) Free radicals in skin and muscle: damaging agents or signals for adaptation? Proc Nutr Soc 58:673–676

    Article  PubMed  CAS  Google Scholar 

  4. Akaike T (2001) Role of free radicals in viral pathogenesis and mutation. Rev Med Virol 11:87–101

    Article  PubMed  CAS  Google Scholar 

  5. Halliwell B (2007) Biochemistry of oxidative stress. Biochem Soc Trans 35:1147–1150

    Article  PubMed  CAS  Google Scholar 

  6. Valko M, Izakovic M, Mazur M, Rhodes CJ, Telser J (2004) Role of oxygen radicals in DNA damage and cancer incidence. Mol Cell Biochem 266:37–56

    Article  PubMed  CAS  Google Scholar 

  7. Maeda H, Akaike T (1998) Nitric oxide and oxygen radicals in infection, inflammation, and cancer. Biochemistry (Mosc) 63:854–865

    CAS  Google Scholar 

  8. Stone V, Johnston H, Clift MJ (2007) Air pollution, ultrafine and nanoparticle toxicology: cellular and molecular interactions. IEEE Trans Nanobioscience 6:331–340

    Article  PubMed  Google Scholar 

  9. Zastrow L, Groth N, Klein F, Kockott D, Lademann J, Renneberg R, Ferrero L (2009) The missing link–light-induced (280–1,600 nm) free radical formation in human skin. Skin Pharmacol Physiol 22:31–44

    Article  PubMed  CAS  Google Scholar 

  10. Sander CS, Hamm F, Elsner P, Thiele JJ (2003) Oxidative stress in malignant melanoma and non-melanoma skin cancer. Br J Dermatol 148:913–922

    Article  PubMed  CAS  Google Scholar 

  11. Thiele JJ, Schroeter C, Hsieh SN, Podda M, Packer L (2001) The antioxidant network of the stratum corneum. Curr Probl Dermatol 29:26–42

    PubMed  CAS  Google Scholar 

  12. Darvin M, Zastrow L, Sterry W, Lademann J (2006) Effect of supplemented and topically applied antioxidant substances on human tissue. Skin Pharmacol Physiol 19:238–247

    Article  PubMed  CAS  Google Scholar 

  13. Darvin ME, Haag SF, Meinke MC, Sterry W, Lademann J (2011) Determination of the influence of ir radiation on the antioxidative network of the human skin. J Biophotonics 4:21–29

    Article  PubMed  CAS  Google Scholar 

  14. Darvin ME, Zastrov L, Gonchukov SA, Lademann J (2009) Influence of IR radiation on the carotenoid content in human skin. Optics and Spectroscopy 107(6):917–920

    Google Scholar 

  15. Bernstein PS, Yoshida MD, Katz NB, McClane RW, Gellermann W (1998) Raman detection of macular carotenoid pigments in intact human retina. Invest Ophthalmol Vis Sci 39:2003–2011

    Google Scholar 

  16. Hata TR, Scholz TA, Ermakov IV, McClane RW, Khachik F, Gellermann W, Pershing LK (2000) Non-invasive raman spectroscopic detection of carotenoids in human skin. J Invest Dermatol 115:441–448

    Article  PubMed  CAS  Google Scholar 

  17. Ermakov IV, Ermakova MR, Gellermann W, Lademann J (2004) Noninvasive selective detection of lycopene and beta-carotene in human skin using raman spectroscopy. J Biomed Opt 9:332–338

    Article  PubMed  CAS  Google Scholar 

  18. Darvin ME, Gersonde I, Albrecht H, Gonchukov SA, Sterry W, Lademann J (2005) Determination of beta carotene and lycopene concentrations in human skin using resonance raman spectroscopy. Laser Phys 15:295–299

    CAS  Google Scholar 

  19. Darvin ME, Gersonde I, Meinke M, Sterry W, Lademann J (2005) Non-invasive in vivo determination of the carotenoids beta-carotene and lycopene concentrations in the human skin using the raman spectroscopic method. J Phys D: Appl Phys 38:2696–2700

    Article  CAS  Google Scholar 

  20. Darvin ME, Gersonde I, Albrecht H, Meinke M, Sterry W, Lademann J (2006) Non-invasive in vivo detection of the carotenoid antioxidant substance lycopene in the human skin using the resonance raman spectroscopy. Laser Phys Lett 3:460–463

    Article  CAS  Google Scholar 

  21. Darvin ME, Gerzonde I, Ey S, Brandt NN, Albrecht H, Gonchukov SA, Sterry W, Lademann J (2004) Noninvasive detection of beta-carotene and lycopene in human skin using Raman spectroscopy. Laser Phys 14(2):231–233

    Google Scholar 

  22. Darvin ME, Sandhagen C, Koecher W, Sterry W, Lademann J, Meinke MC (2012) Comparison of two methods for non-invasive determination of carotenoids in human and animal skin: Raman spectroscopy versus reflection spectroscopy. J Biophotonics. 5(7):550–558

    Google Scholar 

  23. Herrling T, Fuchs J, Rehberg J, Groth N (2003) Uv-induced free radicals in the skin detected by esr spectroscopy and imaging using nitroxides. Free Radic Biol Med 35:59–67

    Google Scholar 

  24. Fuchs J, Groth N, Herrling T (1998) Cutaneous tolerance to nitroxide free radicals in human skin. Free Radic Biol Med 24:643–648

    Article  PubMed  CAS  Google Scholar 

  25. Haag SF, Chen M, Peters D, Keck CM, Taskoparan B, Fahr A, Teutloff C, Bittl R, Lademann J, Schafer-Korting M, Meinke MC (2011) Nanostructured lipid carriers as nitroxide depot system measured by electron paramagnetic resonance spectroscopy. Int J Pharm 421:364–369

    Article  PubMed  CAS  Google Scholar 

  26. Haag SF, Bechtel A, Darvin ME, Klein F, Groth N, Schafer-Korting M, Bittl R, Lademann J, Sterry W, Meinke MC (2010) Comparative study of carotenoids, catalase and radical formation in human and animal skin. Skin Pharmacol Physiol 23:306–312

    Article  PubMed  CAS  Google Scholar 

  27. Meinke MC, Haag SF, Schanzer S, Groth N, Gersonde I, Lademann J (2011) Radical protection by sunscreens in the infrared spectral range. Photochem Photobiol 87:452–456

    Article  PubMed  CAS  Google Scholar 

  28. Fuchs J, Groth N, Herrling T (2002) In vivo measurement of oxidative stress status in human skin. Methods Enzymol 352:333–339

    PubMed  CAS  Google Scholar 

  29. Haag SF, Taskoparan B, Darvin ME, Groth N, Lademann J, Sterry W, Meinke MC (2011) Determination of the antioxidative capacity of the skin in vivo using resonance raman and electron paramagnetic resonance spectroscopy. Exp Dermatol 20:483–487

    Article  PubMed  CAS  Google Scholar 

  30. Meinke MC, Darvin ME, Vollert H, Lademann J (2010) Bioavailability of natural carotenoids in human skin compared to blood. Eur J Pharm Biopharm 76:269–274

    Article  PubMed  CAS  Google Scholar 

  31. Blume-Peytavi U, Rolland A, Darvin ME, Constable A, Pineau I, Voit C, Zappel K, Schafer-Hesterberg G, Meinke M, Clavez RL, Sterry W, Lademann J (2009) Cutaneous lycopene and beta-carotene levels measured by resonance Raman spectroscopy: high reliability and sensitivity to oral lactolycopene deprivation and supplementation. Eur J Pharm Biopharm 73:187–194

    Article  PubMed  CAS  Google Scholar 

  32. Darvin ME, Fluhr JW, Schanzer S, Richter H, Patzelt A, Meinke MC, Zastrow L, Golz K, Doucet O, Sterry W, Lademann J (2011) Dermal carotenoid level and kinetics after topical and systemic administration of antioxidants: enrichment strategies in a controlled in vivo study. J Dermatol Sci 64:53–58

    Article  PubMed  CAS  Google Scholar 

  33. Meinke MC, Friedrich A, Tscherch K, Haag SF, Darvin ME, Vollert H, Groth N, Lademann J, Rohn S (2013) Influence of dietary carotenoids on radical scavenging capacity of the skin and skin lipids. Eur J Pharm Biopharm 84(2):365–373

    Article  PubMed  CAS  Google Scholar 

  34. Lademann J, Caspers PJ, van der Pol A, Richter H, Patzelt A, Zastrow L, Darvin M, Sterry W, Fluhr JW (2009) In vivo raman spectroscopy detects increased epidermal antioxidative potential with topically applied carotenoids. Laser Phys Lett 6:76–79

    Article  CAS  Google Scholar 

  35. Fluhr JW, Caspers P, van der Pol JA, Richter H, Sterry W, Lademann J, Darvin ME (2011) Kinetics of carotenoid distribution in human skin in vivo after exogenous stress: disinfectant and wira-induced carotenoid depletion recovers from outside to inside. J Biomed Opt 16:035002

    Article  PubMed  Google Scholar 

  36. Darvin ME, Patzelt A, Knorr F, Blume-Peytavi U, Sterry W, Lademann J (2008) One-year study on the variation of carotenoid antioxidant substances in living human skin: influence of dietary supplementation and stress factors. J Biomed Opt 13:044028-(1–9)

    Article  PubMed  Google Scholar 

  37. Meinke MC, Taskoparan B, Gersonde I, Lademann J, Darvin ME (2011) Influence on the carotenoid levels of skin arising from age, gender, body mass index in smoking/non-smoking individuals. Free Radic Antioxid 1:15–20

    Google Scholar 

  38. Mayne ST, Cartmel B, Scarmo S, Lin H, Leffell DJ, Welch E, Ermakov I, Bhosale P, Bernstein PS, Gellermann W (2010) Noninvasive assessment of dermal carotenoids as a biomarker of fruit and vegetable intake. Am J Clin Nutr 92:794–800

    Article  PubMed  CAS  Google Scholar 

  39. Tucker KL, Chen H, Vogel S, Wilson PW, Schaefer EJ, Lammi-Keefe CJ (1999) Carotenoid intakes, assessed by dietary questionnaire, are associated with plasma carotenoid concentrations in an elderly population. J Nutr 129:438–445

    PubMed  CAS  Google Scholar 

  40. Brady WE, Mares-Perlman JA, Bowen P, Stacewicz-Sapuntzakis M (1996) Human serum carotenoid concentrations are related to physiologic and lifestyle factors. J Nutr 126:129–137

    PubMed  CAS  Google Scholar 

  41. Darvin ME, Gersonde I, Albrecht H, Sterry W, Lademann J (2006) In vivo raman spectroscopic analysis of the influence of uv radiation on carotenoid antioxidant substance degradation of the human skin. Laser Phys 16:833–837

    Article  CAS  Google Scholar 

  42. Ribaya-Mercado JD, Garmyn M, Gilchrest BA, Russell RM (1995) Skin lycopene is destroyed preferentially over beta-carotene during ultraviolet irradiation in humans. J Nutr 125:1854–1859

    PubMed  CAS  Google Scholar 

  43. Di Mascio P, Kaiser S, Sies H (1989) Lycopene as the most efficient biological carotenoid singlet oxygen quencher. Arch Biochem Biophys 274:532–538

    Article  PubMed  Google Scholar 

  44. Darvin ME, Gersonde I, Albrecht H, Zastrow L, Sterry W, Lademann J (2007) In vivo raman spectroscopic analysis of the influence of ir radiation on the carotenoid antioxidant substances beta-carotene and lycopene in the human skin. Formation of free radicals. Laser Phys Lett 4:318–321

    Article  CAS  Google Scholar 

  45. Darvin ME, Haag S, Meinke M, Zastrow L, Sterry W, Lademann J (2010) Radical production by infrared a irradiation in human tissue. Skin Pharmacol Physiol 23:40–46

    Article  PubMed  CAS  Google Scholar 

  46. Darvin ME, Haag SF, Lademann J, Zastrow L, Sterry W, Meinke MC (2010) Formation of free radicals in human skin during irradiation with infrared light. J Invest Dermatol 130:629–631

    Article  PubMed  CAS  Google Scholar 

  47. Darvin M, Patzelt A, Gehse S, Schanzer S, Benderoth C, Sterry W, Lademann J (2008) Cutaneous concentration of lycopene correlates significantly with the roughness of the skin. Eur J Pharm Biopharm 69:943–947

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Charité – Universitätsmedizin Berlin, Charitéplatz 1, 10117, Berlin, Germany

    J. Lademann, M. E. Darvin, J. W. Fluhr & M. C. Meinke

Authors
  1. J. Lademann
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  2. M. E. Darvin
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  3. J. W. Fluhr
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  4. M. C. Meinke
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Correspondence to J. Lademann .

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Editors and Affiliations

  1. Clinical Dermatology, San Gallicano Dermatological Institute, Rome, Roma, Italy

    Enzo Berardesca

  2. Dermatology, University of California San Francisco, San Francisco, CA, California, USA

    Howard I. Maibach

  3. Proderm, Schenefeld/Hamburg, Germany

    Klaus-Peter Wilhelm

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Lademann, J., Darvin, M.E., Fluhr, J.W., Meinke, M.C. (2014). Interaction Between Free Radicals and Antioxidants in Human Skin. In: Berardesca, E., Maibach, H., Wilhelm, KP. (eds) Non Invasive Diagnostic Techniques in Clinical Dermatology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32109-2_18

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  • DOI: https://doi.org/10.1007/978-3-642-32109-2_18

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