Abstract
The aim of this work was to prepare a material for biodiesel. Filamentous cyanobacterium Anabaena 7120 was mutated by DNA recombination technique. Anti and sense vectors, harbering phosphoenolpyruvate carboxylase (PEPC) gene fragment pepcA, were constructed and transformed Anabaena 7120. The down-regulating mutant decreased PEPC activity to 12.9% ofthat in wild type cells, and its lipid content elevated 54.7% of that in wild type cells. The lipid content may increase to 54.15% of dry weigh. The mutant elevated net photosynthetic activity 51%–77% higher than that in wild type cells, and the activity in BG11(-N) medium was higher than in BG11(+N) medium. Also, its tolerance to higher light intensity and temperature was enhanced. From these data, the mutant contained more lipid, grew fast, tolerated high light intensity and temperature, harvested easily and the species of cyanobacterium was no toxic. So, using the mutant as a feedstock for biodiesel production may reduce the cost.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bligh EG, Dyer WJ (1959) A Rapid Method of Total Lipid Extraction and Purification. Canadian Journal of Physiology and Pharmacology 37: 911–917
Bradford MM (1976) A Rapid and Sensitive Method for the Quantification of Microgram Uantities of Protein Utilizing the Principle of Protein Dye Binding. Ann. Biochem 72: 248–254
Canovas JL, Kornberg HL (1996) Proc. Roy. Soc. Ser. B Biol. Sci. 165: 189–205
Chisti Y (2007) Biodiesel from Microalgae. Biotechnology Advances 25: 294–306
Dunahay TG, Jarvis EE, Dais SS, Roessler PG (1996) Manipulation of Microalgal Lipid Production Using Genetic Engineering Applied Biochemistry and Biotechnology-Part A Enzyme Engineering and Biotechnology 57: 223–231
Hou LJ, Wang XK, Shi DJ (2008a) Bioinformatical Analysis of Phosphoenol-Pyruvate Carboxylase in Cyanobacteria. Biotech. Bull (in Chinese) 4: 149–154
Hou LJ, Shi DJ, Cai ZF, Song DH, Wang XK (2008b) Regulation of Lipid Synthesis in Escherichia Coli by Cyanobacterial pepcA. J. Chinese Biotech 28(5): 52–58
Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seribert M, Darzins A (2008) Microalgal Triacylglycerols as Feedstocks for Biofuel Production: Perspectives and Advances. Plant Journal 54: 621–639
Izui I, Matzumura H, Furumoto T, Kai Y (2004) Phosphoenolpyruvate Carboxylase: a New Era of Structural Biology. Annu. Rev. Plant Bio 55: 69–84
Peterhansei C, Niessen M, Kebeish RM (2008) Metabolic Engineering Towards the Enhancement of Photosynthesis. Photochem and Photobiol 84: 1317–1323
Qing JD, Shi DJ, Xu XD, Zhang JD, Guo PZ, Tang PS (1998) Construction of Antisense glnA Expression System in Anabaena 7120. Acta phytophysiol. Sini 24(3): 225–232
Qing JD, Shao N, Shi DJ, Xu XD, Zhang JD, Guo PZ, Wang WQ, Tang PS (1999) Construction of Secreting Ammonium Mutant from Synechococcus 7942 and Its Glutamine Synthetase Activity, Photosynthesis and Growth. Acta Bot. Sini 41(1): 65–70
Sambrook J, Fritsch EF, Maniatis T (2001) Molecular Cloning. A Laboratory Manual 3rd edition, Cold Spring Harbor Laboratory Press
Sheehan J, Dunahay T, Benemann J, Roessler P (1998) A Look Back at the US Department of Energy’s Aquatic Species Program-Biodiesel from Algae. National Renewable Energy Laborattory, Golden, Colorado
Shi DJ (1987) Energy Metabolism and Structure of Immobilized Cyanobacterium Anabaena Azollae. Ph.D thesis, King’s College London
Shi DJ, Brouers M, Hall DO, Robins RJ (1987) The Effects of Immobilization on the Biochemical, Physiological and Morphological Features of Anabaena azollae. Planta 172: 298–308
Shi DJ (2011) Construction of Cyanobacterial Cell Factories. Science & Technology Stimulating Development (in press). (in Chinese)
Zhu XG, Long SP, Ort DR (2010) Improving Photosynthetic Efficiency for Greater Yield. Annu Rev. Plant Biol 61: 235–261
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2013 Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Hou, L., Jia, X., Shi, D., Wang, X. (2013). Expression of PEPC Gene, Lipid Content and Photosynthesis in Anabaena 7120. In: Photosynthesis Research for Food, Fuel and the Future. Advanced Topics in Science and Technology in China. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32034-7_70
Download citation
DOI: https://doi.org/10.1007/978-3-642-32034-7_70
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-32033-0
Online ISBN: 978-3-642-32034-7
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)