Abstract
Members of the rhodophytan order Cyanidiales are unique among phototrophs in their ability to live in extremely low pH levels and moderately high temperatures. The photosynthetic apparatus of the red alga Cyanidioschyzon merolae represents an intermediate type between cyanobacteria and higher plants, suggesting that this alga may provide the evolutionary link between prokaryotic and eukaryotic phototrophs. While red algal PSI resembles that of the higher plants, the PSII complex is more reminiscent of the cyanobacterial ancestor in that it contains phycobilisomes as the light-harvesting system instead of Chla/b binding proteins of green algae and higher plants, as well as the PsbU and PsbV subunits stabilising the oxygen evolving complex (OEC). The most remarkable feature of the red algal PSII is the presence of the fourth extrinsic protein of 20 kDa (PsbQ’) which is not found in the cyanobacterial OEC and which is distantly related with the green algal PsbQ. This feature together with some differences in the structural cooperation between the OEC subunits suggests that the lumenal side of red algal PSII may vary from the prokaryotic ancestor. In order to elucidate the structural differences between cyanobacterial and eukaryotic PSII, we have isolated highly active and stable dimeric complexes of the C. merolae PSII and subjected them to high throughput crystallization and mass spectrometry analyses. Here we report the full subunit composition and preliminary results of 3D crystallization of the dimeric C. merolae PSII.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Adachi H, Umena Y, Enami I, Henmi T, Kamiya N, Shen JR (2009) Towards Structural Elucidation of Eukaryotic Photosystem II: Purification, Crystallization and Preliminary X-Ray Diffraction Analysis of Photosystem II from a Red Alga. Biochim. Biophys. Acta 1787: 121–128
Ciniglia C, Yoon HS, Pollio A, Pinto G, Bhattacharya D (2004) Hidden Biodiversity of the Extremophilic Cyanidiales Red Algae. Mol. Ecol. 13: 1827–1838
Dau H, Grundmeier A, Loja P, Haumann M (2008) On the Structure of the Manganese Complex of Photosystem II: Extended-Range EXAFS Data and Specific Atomic-Resolution Models for Four S-States. Philos Trans R Soc Lond B Biol Sci. 363: 1237–1243
Dobáková M, Sobotka R, Tichý M, Komenda J (2009) Psb28 Protein Is Involved in the Biogenesis of the Photosystem II Inner Antenna CP47 (PsbB) in the Cyanobacterium Synechocystis sp. PCC 6803. Plant Physiol. 149: 1076–1086
Enami I, Okumura A, Nagao R, Suzuki T, Iwai M, Shen JR (2008) Structures and Functions of the Extrinsic Proteins of Photosystem II from Different Species. Photosynth. Res. 98: 349–363
Ferreira KN, Iverson TM, Maghlaoui K, Iwata S, Barber J (2004) Architecture of the Photosynthetic Oxygen-Evolving Center. Science 303: 1831–1838
Gardian Z, Bumba L, Schrofel A, Herbstova M, Nebesarova J, Vacha F (2007) Organisation of Photosystem I and Photosystem II in Red Alga Cyanidium Caldarium: Encounter of Cyanobacterial and Higher Plant Concepts. Biochim. Biophys. Acta 1767: 725–731
Guskov A, Kern J, Gabdulkhakov A, Broser M, Zouni A, Saenger W (2009) Cyanobacterial Photosystem II at 2.9-A Resolution and the Role of Quinones, Lipids, Channels and Chloride. Nat. Struct. Mol. Biol. 16: 334–342
Kamiya N, Shen JR (2003) Crystal Structure of Oxygen-Evolving Photosystem II from Thermosynechococcus Vulcanus at 3.7-A Resolution. Proc. Natl Acad. Sci. 100: 98–103
Kargul J, Maghlaoui K, Murray JW, Deak Z, Vass I, Boussac A, Rutherford AW, Barber J (2007) Purification, Crystallization and X-Ray Diffraction Analyses of the T. Elongatus PSII Core Dimer with Strontium Replacing Calcium in the Oxygen-Evolving Complex. Biochim. Biophys. Acta 1767: 404–413
Loll B, Kern J, Saenger W, Zouni A, Biesiadka J (2005) Towards Complete Cofactor Arrangement in the 3.0 A Resolution Structure of Photosystem II. Nature 438: 1040–1044
Murray JW, Maghlaoui K, Kargul J, Ishida N, Lai T-L, Rutherford AW, Sugiura M, Boussac A, Barber J (2008a) X-Ray Crystallography Identifies Two Chloride Binding Sites in the Oxygen Evolving Centre of Photosystem II. Energy Environ. Sci. I: 161–166
Murray JW, Maghlaoui K, Kargul J, Sugiura M, Barber J (2008b) Analysis of Xenon Binding to Photosystem II by X-ray Crystallography. Photosynth. Res. 98: 523–527
Nowaczyk MM, Hebeler R, Schlodder E, Meyer HE, Warscheid B, Rögner M (2006) Psb27, a Cyanobacterial Lipoprotein, Is Involved in the Repair Cycle of Photosystem II. Plant Cell 18: 3121–3131
Nozaki H, Matsuzaki M, Takahara M, Misumi O, Kuroiwa H, Hasegawa M, Shin-i T, Kohara Y, Ogasawara N, Kuroiwa T (2003) The Phylogenetic Position of Red Algae Revealed by Multiple Nuclear Genes from Mitochondria-Containing Eukaryotes and an Alternative Hypothesis on the Origin of Plastids. J. Mol. Evol. 56: 485–497
Ohta H, Suzuki T, Ueno M, Okumura A, Yoshihara S, Shen JR, Enami I (2003) Extrinsic Proteins of Photosystem II: an Intermediate Member of PsbQ Protein Family in Red Algal PSII. Eur. J. Biochem. 270: 4156–4163
Siegbahn PE (2009) Structures and Energetics for O2 fFormation in Photosystem II. Acc Chem Res. 42: 1871–1880
Sproviero EM, Shinopoulos K, Gascón JA, McEvoy JP, Brudvig GW, Batista VS (2008) QM/MM Computational Studies of Substrate Water Binding to the Oxygen-Evolving Centre of Photosystem II. Philos. Trans. R Soc. Lond. B Biol. Sci. 363: 1149–1156
Yano J, Kern J, Sauer K, Latimer MJ, Pushkar Y, Biesiadka J, Loll B, Saenger W, Messinger J, Zouni A, Yachandra VK. (2006) Where Water Is Oxidized to Dioxygen: Structure of the Photosynthetic Mn4Ca Cluster. Science 314: 821–825
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2013 Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Kargul, J., Boehm, M., Morgner, N., Robinson, C.V., Nixon, P.J., Barber, J. (2013). Compositional and Structural Analyses of the Photosystem II Isolated from the Red Alga Cyanidioschyzon Merolae . In: Photosynthesis Research for Food, Fuel and the Future. Advanced Topics in Science and Technology in China. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32034-7_12
Download citation
DOI: https://doi.org/10.1007/978-3-642-32034-7_12
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-32033-0
Online ISBN: 978-3-642-32034-7
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)