The Biology and Genomic Localization of Cytosine Modifications

  • Gordon R. McInroy
  • Neil M. Bell
  • Gabriella Ficz
  • Shankar Balasubramanian
  • Wolf Reik
  • Eun-Ang RaiberEmail author
Part of the Epigenetics and Human Health book series (EHH)


Epigenetic mechanisms describe gene expression states that are somatically heritable but do not involve changes in the underlying DNA sequence of the organism. Epigenetic changes are mediated by dynamic chemical modifications of DNA and histone proteins within chromatin. These modifications play a central role in the regulation of processes such as transcription, replication, and DNA repair and represent a unique profile for each cell type. In this chapter we will focus on DNA modifications, particularly on the 5′ position of cytosine, their potential implications in epigenetically controlled mechanisms, and furthermore discuss the technical challenges that are associated with the methods used to study these modifications.


Bisulfite Sequencing Reduce Representation Bisulfite Sequencing 5hmC Level Primordial Germ Cell SMRT Sequencing 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Gordon R. McInroy
    • 1
  • Neil M. Bell
    • 1
  • Gabriella Ficz
    • 2
  • Shankar Balasubramanian
    • 1
    • 3
    • 4
  • Wolf Reik
    • 5
    • 6
  • Eun-Ang Raiber
    • 1
    Email author
  1. 1.Department of ChemistryUniversity of CambridgeCambridgeUK
  2. 2.Centre for Haemato-OncologyBarts Cancer InstituteLondonUK
  3. 3.Cancer Research UK Cambridge Institute, Li Ka Shing CentreCambridgeUK
  4. 4.School of Clinical MedicineThe University of CambridgeCambridgeUK
  5. 5.Centre for Trophoblast ResearchUniversity of CambridgeCambridgeUK
  6. 6.Epigenetics ProgrammeThe Babraham InstituteCambridgeUK

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