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Use of Metabolomics and Transcriptomics to Gain Insights into the Regulation and Biosynthesis of Medicinal Compounds: Hypericum as a Model

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Biotechnology for Medicinal Plants

Abstract

Medicinal plants have been used in alternative medicine for thousands of years and produce many unique classes of compounds. These compounds have effects on human and cultured cells that include but are not limited to anti-viral, anti-bacterial, anti-depressive, and anti-oxidant properties. The levels of accumulation and the ratios of specialized metabolites are highly influenced by factors including genotype, natural variation due to biotic and abiotic cues, optimization of compounds, the intermediate chemicals within the pathways, sites of synthesis, and the biosynthetic genes dictating their synthesis. All these factors impact the quality of propagated material. This chapter gives an overview of (1) understanding the natural variation in bioactive compounds across different treatments and tissues; (2) an approach for finding putative biosynthetic genes utilizing transcriptomic and metabolomic technologies; and (3) the positive and negative effects on humans. These are illustrated with examples from the genus Hypericum.

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Acknowledgments

This publication was made possible in part by grant 9P50AT004155-06 from National Center for Complementary and Alternative Medicine and Office of Dietary Supplements, and grant NIGM-99511 from National Institute of General Medical Sciences, National Institutes of Health, its contents are solely the responsibility of the authors; and by the Genetics Program, Iowa State University (to MCC). We thank Dr. Heather Babka, Iowa State University, for training MC in H. gentianoides biology, Dr. Robin Buell and Dr. Elsa Gongora Castillo, Michigan State University, for insightful discussions into RNA-seq technologies. We are grateful to Dr. Mark Wiederlichtner for helpful discussions and for defined accession seeds of H. gentianoides (Ames 27729 (http://www.ars-grin.gov/cgi-bin/npgs/acc/display.pl?1668188)).

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  1. Department of Genetics, Developmental, and Cell Biology, Iowa State University, 433 Bessey Hall, Ames, IA, USA

    Matthew C. Crispin

  2. Department of Genetics, Developmental, and Cell Biology, Iowa State University, 441 Bessey Hall, Ames, IA, 50010, USA

    Eve Syrkin Wurtele

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Correspondence to Eve Syrkin Wurtele .

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  1. , National Center for Natural Product Rese, University of Mississippi, Coy Waller Complex 135, University, 38677, Mississippi, USA

    Suman Chandra

  2. Nat Center Natural Product Research, School of Pharmacy, University of Mississippi, COY WALLER COMPLEX 135, UNIVERSITY, 38677, Mississippi, USA

    HEMANT LATA

  3. Amity Institute of Microbial Sciences, Amity University Uttar Pradesh, Noida, 201303, Uttar Pradesh, India

    Ajit Varma

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Crispin, M.C., Wurtele, E.S. (2013). Use of Metabolomics and Transcriptomics to Gain Insights into the Regulation and Biosynthesis of Medicinal Compounds: Hypericum as a Model. In: Chandra, S., LATA, H., Varma, A. (eds) Biotechnology for Medicinal Plants. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-29974-2_17

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