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Transcriptome/Proteome Analysis of Corynebacterium glutamicum

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Corynebacterium glutamicum

Part of the book series: Microbiology Monographs ((MICROMONO,volume 23))

Abstract

Our understanding of Corynebacterium glutamicum has been revolutionized since its genome sequence, whole-genome DNA microarrays for transcriptome analysis, and proteomics techniques have become available. This chapter describes how DNA microarray-based transcriptome analyses as well as cytosolic and membrane proteomics have expedited the knowledge about the physiology and metabolic regulation of C. glutamicum with respect to fundamental and applied research. Recent examples of employing transcriptome analysis to study stimulons, transcriptional regulators, in particular of carbon metabolism and the stress response, to characterize in vivo-evolved strains, for pathway identification, genome instabilities, and strain development for amino acid production are given along with an outlook into future development of transcriptomics due to technical developments. The status quo of C. glutamicum proteomics by classical 2D gel electrophoresis and more recent techniques also allowing the study of membrane proteins are described. Emphasis is given to the posttranslational modification of C. glutamicum proteins by phosphorylation, glycosylation, and N-terminal processing. Selected examples of studying protein–DNA and protein–protein interactions are also discussed.

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Acknowledgments

We thank Kirsten Bräker (Forschungszentrum Juelich GmbH) for providing Fig. 1 and Nadine Dobler (Forschungszentrum Juelich GmbH) and Alexander Schliep (Rutgers State University of New Jersey) for the help with C. glutamicum tiling arrays. Part of the work in the Wendisch laboratory was supported through grants WE 23020/2-1 (DFG, ESF), 0315589 G (BMBF), and ERA-IB (22009508).

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  1. Faculty of Biology & CeBiTec, University of Bielefeld, 100131, 33501, Bielefeld, Germany

    Volker F. Wendisch

  2. Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Juelich GmbH, 52425, Juelich, Germany

    Tino Polen

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Correspondence to Volker F. Wendisch .

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  1. Technology for the Earth (RITE), Microbiology Research Group, Research Institute of Innovative, Kizu-cho 9-2, Soraku-gun, Kyoto, 619-0292, Japan

    Hideaki Yukawa

  2. Technology for the Earth (RITE), Research Institute of Innovative, Kizugawadai, Kizu-cho 9-2, Soraku-gun, Kyoto, 619-0292, Japan

    Masayuki Inui

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Wendisch, V.F., Polen, T. (2013). Transcriptome/Proteome Analysis of Corynebacterium glutamicum . In: Yukawa, H., Inui, M. (eds) Corynebacterium glutamicum. Microbiology Monographs, vol 23. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-29857-8_6

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