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Genome Engineering of Corynebacterium glutamicum

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Corynebacterium glutamicum

Part of the book series: Microbiology Monographs ((MICROMONO,volume 23))

Abstract

As a direct consequence of the recent advances in DNA sequencing technologies, complete genome sequences of more than 1,200 bacterial species have already been deciphered and they form an important resource for understanding the diversity of bacterial metabolic systems. Manipulation of bacterial genome sequences by integration, replacement, and disruption of individual genes has, in parallel, become a powerful strategy to improve bacterial traits. Regarding Corynebacterium glutamicum, whole genome sequences of two strains, namely R (3,314,179 bp) and ATCC 13032 (3,309,401 bp or 3,282,708 bp), have been determined and strain reconstruction studies initiated. Several techniques for genome-wide genetic manipulations using transposons, DNA recombinase, and homologous recombination reactions have been developed. These advances are particularly important because C. glutamicum has a long history of applications for the production for various commodity and fine chemicals. Armed with the microbe’s complete sequence, improvement and tailoring of its properties using genome engineering techniques continue to help facilitate the identification of metabolic bottlenecks and, consequently, their resolution. This in turn enhances the intrinsic characteristics of this bacterium as an industrial workhorse. In this chapter, recently developed techniques that enable to manipulate the C. glutamicum genome are summarized.

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Acknowledgments

We wish to thank Dr. C. Omumasaba (internal) for the critical reading of the manuscript.

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  1. Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, Kizugawa, Kyoto, 619-0292, Japan

    Nobuaki Suzuki & Masayuki Inui

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  1. Nobuaki Suzuki
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Correspondence to Masayuki Inui .

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  1. Technology for the Earth (RITE), Microbiology Research Group, Research Institute of Innovative, Kizu-cho 9-2, Soraku-gun, Kyoto, 619-0292, Japan

    Hideaki Yukawa

  2. Technology for the Earth (RITE), Research Institute of Innovative, Kizugawadai, Kizu-cho 9-2, Soraku-gun, Kyoto, 619-0292, Japan

    Masayuki Inui

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Suzuki, N., Inui, M. (2013). Genome Engineering of Corynebacterium glutamicum . In: Yukawa, H., Inui, M. (eds) Corynebacterium glutamicum. Microbiology Monographs, vol 23. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-29857-8_3

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