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Corynebacterium glutamicum pp 51–88Cite as

Promoters and Plasmid Vectors of Corynebacterium glutamicum

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Part of the book series: Microbiology Monographs ((MICROMONO,volume 23))

Abstract

The promoters and plasmids of Corynebacterium glutamicum can be viewed as subjects for genetic examination, tools for studies of gene expression, and a means for the improvement of industrial strains. Various classes of C. glutamicum promoters, their specific functions in gene expression control, and methods for their analysis are described in this review. C. glutamicum promoters recognized by RNA polymerase with a specific sigma factor (SigA, SigB, SigH, or SigM) were localized and their consensus sequences determined. Experimental localization of transcriptional start points revealed complex gene expression regulation features, such as overlapping multiple promoters. An overview of native plasmids used for the construction of C. glutamicum vectors and examples of vectors applied in genetic analysis are provided. In addition to basic cloning vectors, special-purpose vectors are described. This includes promoter-probe vectors for the analysis of promoter activity profiles and various regulatory mechanism and expression vectors, which carry promoters as efficient signals for gene expression.

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Acknowledgments

Work in the authors’ laboratory was supported by grant 204/09/J015 from the Scientific Council of the Czech Republic.

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  1. Institute of Microbiology, Academy of Sciences of the Czech Republic, v. v. i., Vídeňská 1083, 14220, Prague 4, Czech Republic

    Miroslav Pátek & Jan Nešvera

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  1. Miroslav Pátek
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  2. Jan Nešvera
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Correspondence to Miroslav Pátek .

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  1. Technology for the Earth (RITE), Microbiology Research Group, Research Institute of Innovative, Kizu-cho 9-2, Soraku-gun, Kyoto, 619-0292, Japan

    Hideaki Yukawa

  2. Technology for the Earth (RITE), Research Institute of Innovative, Kizugawadai, Kizu-cho 9-2, Soraku-gun, Kyoto, 619-0292, Japan

    Masayuki Inui

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Pátek, M., Nešvera, J. (2013). Promoters and Plasmid Vectors of Corynebacterium glutamicum . In: Yukawa, H., Inui, M. (eds) Corynebacterium glutamicum. Microbiology Monographs, vol 23. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-29857-8_2

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